Abstract:

Objective  To explore expressions of GABAAR β2 and γ2 subunits in the inferior colliculus of newborn rats. Methods  mRNAs expressions of GABAAR β2 and γ2 subunits in the inferior colliculus of newborn rats from 1 to 16 days were quantitated by half quantitation PT-PCR and located by in situ hybridization. The PCR were done with interior control GAPDH. The gray scales of the electrophoresis products of PCR were observed and analyzed under an ultraviolet analytical system. In situ hybridization was done by a non-radioactive oligonucleotide probe and the results were observed by a light microscope. Results  Expressions of GABAAR β2 and γ2 subunits in the inferior colliculus were gradually increased in a nonlinear way.  Expression level was the lowest at day 7, reached a peak at day 13 and dropped. to  a  level similar with that of adult rats at day 16. The differences were significant（P<0.01）. Conclusion  Expressions of GABAAR β2 and γ2 subunits in the inferior colliculus were undulatory and phasic in postnatal rats. The two subunits were co-expressed in the inferior colliculus.

Key words: γ-aminobutyricacid type A receptor; β2 subunit; γ2 subunit; Inferior Colliculus; Rats