JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2010, Vol. 24 ›› Issue (5): 5-.

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Differentially expressed genes in 3 cases of hypopharyngeal carcinoma and  normal tissues by cDNA microarray

 L Mei1， DONG Pin2， SHE Cui-ping3， DU Cui-ping1， LI Yong1， XU Er-dong1   

  1. 1.  Department of Otolaryngology,  First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning, China；
    2. Department of Otolaryngology-Head and Neck Surgery, the First Hospital affiliated to Shanghai Communication
    University， Shanghai 200080， China；
    3.  Department of Otolaryngology-Head and Neck Surgery, Central Hospital of Dalian， Dalian 116033,  Liaoning, China
  • Received:2010-07-11 Revised:2010-09-11 Online:2010-10-16 Published:2010-10-16

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Abstract:

Objective    To detect differentially expressed genes in hypopharyngeal carcinoma and adjacent normal laryngeal tissues with cDNA microarray. Methods      Three samples of hypopharyngeal carcinoma, pathologically confirmed as squamous cell carcinoma,  were tested.  Total RNAs were extracted and  inversely transcribed to cDNAs.The cDNAs of hypopharyngeal carcinoma were marked by Cy5 and  the cDNAs of normal laryngeal tissues were marked by Cy3. The cDNA probes were then hybridized to DNA microarrays and scanned for fluorescent signals. Differential expression between the two tissues was produced by ImaGene software. The average ratio of gray scale, if equal or more than 2 times,  was considered significant. Results      There were 51 genes significantly expressed in 3 cases. Among them, 39 genes were significantly higher, but 12 genes were significantly lower in hypopharyngeal carcinoma than  normal tisscle.4 new genes were identified. Some highly expressed genes (more than 5 fold difference ) included: 1) Genes of upregulation, such as MMP-1, Integrinβ4, Bcl-2, CXCL13 and PRAME; and 2) Genes of downregulation, such as Egr-1 and MRP1/ CD9. Conclusion     Many genes, which  play important roles in the proliferation, invasion, and metastasis of hypopharyngeal carcinoma, can be picked out by cDNA microarray;  These genes   will serve as targets for further research.

Key words: Hypopharyngeal carcinoma; Squamous cell carcinoma; Gene expression; DNA microarray

CLC Number: 

  • R739.65
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