Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2018, Vol. 32 ›› Issue (6): 98-103.doi: 10.6040/j.issn.1673-3770.0.2018.319

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Protective effect of puerarin on the optic nerve in rats with chronic intraocular hypertension

LIU Bei1, LÜ Bochang1, ZHU Zhongqiao1, SUN Jianhua2   

  1. Reproductive Center, Northwest Women and Childrens Hospital, Xian 710001, Shaanxi, China
  • Published:2018-11-29

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Abstract: Objective To observe the protective effect of Puerarin on optical nerve in rats with chronic intraocular hypertension. Methods Sixty Sprague-Dawley rats were randomly divided into the sham operation, model control, and puerarin groups by the random number table method. Each group contained 20 rats, and the right eye was used as the experimental eye. Chronic intraocular hypertension was established in the model control and puerarin groups by scleral vein cautery; in the sham operation group, the conjunctiva was cut. Puerarin was injected intraperitoneally into rats in the puerarin group(10 mL/kg), while 0.5 mL saline was injected intraperitoneally into rats in the sham operation and model control groups, both before the operation and once daily after the operation for 4 weeks. Intraocular pressure was measured before the operation and 1 day, 3 days, 1 week, 2 weeks, 3 weeks, and 4 weeks after the operation. Rat eyeballs and optic nerve specimens were prepared at 4 weeks after the operation. The retinal thickness was measured by hematoxylin-eosin staining. Ultrastructural changes in optic nerve axons in rats were observed by transmission electron microscopy. The expression of p-AKT and AKT proteins in the retina was analyzed by western blotting. Results There were significant differences with respect to changes in intraocular pressure before and after the operation in the model control and puerarin groups(Fpuerarin=448.22, P<0.001; Fmodel=450.86, P<0.001). There was a significant difference in the intraocular pressure among the three groups at 4 weeks after the operation(F=520.88, P<0.001). At 4 weeks after operation, there were significant differences among the three groups in retinal thickness(F=441.29, P<0.001), in the number of myelinated nerve fibers (F=1 344.31, P<0.001), and in the ratio of p-AKT to AKT(F=90.22, P<0.001). Conclusion Puerarin has a protective effect on the optic nerve in rats with chronic intraocular hypertension.

Key words: Puerarin, Ocular hypertension, Optic nerve protection, Herbal medicine

CLC Number: 

  • R774
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