Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2022, Vol. 36 ›› Issue (2): 32-39.doi: 10.6040/j.issn.1673-3770.0.2021.119

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CHD1L promotes proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells by EMT

LIU Yong1, YUAN Cunli1, CAO Hui1, ZHENG Chengcai1, CHAO Fang2, XU Fenglei3   

  1. 1.Department of Otolaryngology, Rizhao Central Hospital, Rizhao 276800, Shandong, China;
    2.First Operating Room, Rizhao People's Hospital, Rizhao 276826, Shandong, China;
    3. Department of Otolaryngology, Shandong Provincial Hospital, Jinan 250022, Shandong, China
  • Published:2022-04-15

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Abstract: Objective To investigate the mechanism of chromodomain helicase DNA binding protein 1-like gene(CHD1L )in the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells and to provide molecular targets for the treatment of laryngeal squamous cell carcinoma(LSCC). Methods Immunohistochemistry was conducted to measure the expression of CHD1L, E-cadherin and Vimentin in 60 pairs of LSCC and its adjacent normal mucosa. The expression of CHD1L in human bronchial epithelial cell(HBE)and human laryngeal epithelioid carcinoma cell(HEp-2)was detected by Western blotting. CHD1L in HEp-2 cells was knocked out, and Western blotting was used to detect the expression of CHD1L, E-cadherin and Vimentin in HEp-2 cells. The proliferation of cells in each group was detected by CCK-8 kit, and the invasion of cells in each group was detected by Transwell invasion test. Results Immunohistochemical results showed that the positive expression rate of CHD1L in laryngeal squamous cell carcinoma tissue was significantly higher than that of normal paracaner mucosa tissues. In addition, the expression of CHD1L was positively correlated with the degree of differentiation of laryngeal squamous cell carcinoma, the lymph node metastasis, the clinical stage and the expression of Vimentin, but negatively correlated with the expression of E-cadherin(P<0.05). The results of Western blotting showed that the expression of CHD1L in HEp-2 cell was significantly higher than that in HBE cell. CHD1L in HEp-2 cells was knocked down by interfering plasmid in vitro. The proliferation and invasion ability of the HEp-2 cells with CHD1L knockdown in vitro were significantly decreased, while the expression of E-cadherin was significantly increased and the expression of Vimentin was also significantly decreased. Conclusion The high expression of CHD1L in LSCC was closely related to pathological grades, clinical stage and risk of lymph node metastasis of LSCC, suggesting that CHD1L may play an oncogene role in LSCC; CHD1L could promote the proliferation, invasion and metastasis of LSCC cells by promoting the EMT of LSCC cells.

Key words: Laryngeal squamous cell carcinoma, Chromodomain helicase/ATPase DNA binding protein 1-like gene, Epithelial-mesenchymal transition, Proliferation, Invasion, Metastasis

CLC Number: 

  • R739.65
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