Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2025, Vol. 39 ›› Issue (3): 104-114.doi: 10.6040/j.issn.1673-3770.0.2024.011

• Original Article • Previous Articles    

Research on screening the key genes of age-related hearing loss by GEO database

ZHANG Lixia, LI Lin   

  1. Shanxi Bethune Hospital/Shanxi Academy of Medical Sciences/Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan 030032, Shanxi, China
  • Published:2025-06-04

PDF (PC)

78

Abstract: Objective Screen for the key genes of age-related hearing loss(ARHL)using bioinformatics methods, and provide a new theoretical basis for the clinical diagnosis and treatment of ARHL. Methods Two data sets related to ARHL were obtained from the GEO database and differentially expressed genes(DEGs)were analyzed using the GEO2R tool. DEGs were analyzed using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genome(KEGG)enrichment analysis using the DAVID tool, and a protein-protein interaction(PPI)network was constructed based on STRING database and Cytoscape software. The key genes were screened by the CytoHubba plug-in in Cytoscape software. Key genes were verified in the DEAF model mice. Results 100 DEGs(Differentially Expressed Genes)were selected from the GSE127204 and GSE196870datasets, and the enrichment analysis of GO and KEGG found that they were mainly enriched in cytokine-cytokine receptor interaction and other pathways. Key genes were selected by Cytoscape software, including IRF7, PTPRC, CXCL10, IFI44, IFI204, USP18 and CD4. On this basis, RT-qRCR was used to detect the expression of 7 key genes in mouse cochlear tissue, which further verifying that the above 7 genes were highly expressed in age-related deafness. Conclusion In this study, bioinformatics screening combined with RT-qRCR detection of mouse cochlear tissue and other experimental results confirmed that the expression of 7 key genes was significantly increased in age-related hearing loss.This study provides a new idea and theoretical basis for the early diagnosis and clinical treatment of age-related hearing loss.

Key words: Age-related hearing loss, Hub genes, Protein-protein interaction, Enrichment analysis

CLC Number: 

  • R764.43
[1] Hu SY, Sun QR, Xu F, et al. Age-related hearing loss and its potential drug candidates: a systematic review[J]. Chin Med, 2023, 18(1): 121. doi:10.1186/s13020-023-00825-6
[2] Lahlou G, Calvet C, Giorgi M, et al. Towards the clinical application of gene therapy for genetic inner ear diseases[J]. J Clin Med, 2023, 12(3): 1046. doi:10.3390/jcm12031046
[3] Shende SA, Mudar RA. Cognitive control in age-related hearing loss: a narrative review[J]. Hear Res, 2023, 436: 108814. doi:10.1016/j.heares.2023.108814
[4] Yang W, Zhao XL, Chai RJ, et al. Progress on mechanisms of age-related hearing loss[J]. Front Neurosci, 2023, 17: 1253574. doi:10.3389/fnins.2023.1253574
[5] Keithley EM. Pathology and mechanisms of cochlear aging[J]. J Neurosci Res, 2020, 98(9): 1674-1684. doi:10.1002/jnr.24439
[6] Lang HN, Noble KV, Barth JL, et al. The stria vascularis in mice and humans is an early site of age-related cochlear degeneration, macrophage dysfunction, and inflammation[J]. J Neurosci, 2023, 43(27): 5057-5075. doi:10.1523/JNEUROSCI.2234-22.2023
[7] Liu JL, Chen HY, Lin XY, et al. Melatonin suppresses cyclic GMP-AMP synthase-Stimulator of interferon genes signaling and delays the development of hearing loss in the C57BL/6J presbycusis mouse model[J]. Neuroscience, 2023, 517: 84-95. doi:10.1016/j.neuroscience.2023.01.015
[8] Druszczynska M, Godkowicz M, Kulesza J, et al. Cytokine receptors-regulators of antimycobacterial immune response [J]. Int J Mol Sci, 2022, 23(3):1112. doi: 10.3390/ijms23031112
[9] Uraguchi K, Maeda Y, Takahara J, et al. Upregulation of a nuclear factor-kappa B-interacting immune gene network in mice cochleae with age-related hearing loss[J]. PLoS One, 2021, 16(10): e0258977. doi:10.1371/journal.pone.0258977
[10] Yang CH, Hwang CF, Chuang JH, et al. Systemic toll-like receptor 9 agonist CpG oligodeoxynucleotides exacerbates aminoglycoside ototoxicity[J]. Hear Res, 2021, 411: 108368. doi:10.1016/j.heares.2021.108368
[11] Al Barashdi MA, Ali A, McMullin MF, et al. Protein tyrosine phosphatase receptor type C(PTPRC or CD45)[J]. J Clin Pathol, 2021, 74(9): 548-552. doi:10.1136/jclinpath-2020-206927
[12] Tornabene SV, Sato K, Pham L, et al. Immune cell recruitment following acoustic trauma[J]. Hear Res, 2006, 222(1/2): 115-124. doi:10.1016/j.heares.2006.09.004
[13] Singh G, Gaidhane A. A review of sensorineural hearing loss in congenital cytomegalovirus infection[J]. Cureus, 2022, 14(10): e30703. doi:10.7759/cureus.30703
[14] Kve tak D, Jurani Lisni V, Lisni B, et al. NK/ILC1 cells mediate neuroinflammation and brain pathology following congenital CMV infection[J]. J Exp Med, 2021, 218(5): e20201503. doi:10.1084/jem.20201503
[15] Maeda Y, Kariya S, Omichi R, et al. Targeted PCR array analysis of genes in innate immunity and glucocorticoid signaling pathways in mice cochleae following acoustic trauma [J]. Otol Neurotol, 2018, 39(7): 593-600. doi: 10.1097/MAO.0000000000001874
[16] Wang Y, Ma CF, Ma ZB, et al. Identification and clinical correlation analysis of IFI44 in systemic lupus erythematosus combined with bioinformatics and immune infiltration analysis[J]. J Inflamm Res, 2023, 16: 3219-3231. doi:10.2147/JIR.S419880
[17] Baran M, Feriotti C, McGinley A, et al. PYHIN protein IFI207 regulates cytokine transcription and IRF7 and contributes to the establishment of K.pneumoniae infection[J]. Cell Rep, 2023, 42(4): 112341. doi:10.1016/j.celrep.2023.112341
[18] Lee EJ, Kim JY, Yeo JH, et al. ISG15-USP18 dysregulation by oxidative stress promotes IFN-γ secretion from CD8+T Cells in vitiligo[J]. J Invest Dermatol, 2024, 144(2): 273-283. doi:10.1016/j.jid.2023.08.006
[19] Iwai H, Inaba M, van Bui D, et al. Treg and IL-1 receptor type 2-expressing CD4+ T cell-deleted CD4+ T cell fraction prevents the progression of age-related hearing loss in a mouse model[J]. J Neuroimmunol, 2021, 357: 577628. doi:10.1016/j.jneuroim.2021.577628
[1] DONG Lingkang, YU Dongzhen. Trends and projections of the burden of age-related hearing loss in China from 1990 to 2021 [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(3): 61-69.
[2] LI Lin, GAO Zhengwen, CUI Nan, SUN Jianping, HUANG Xianming, CUI Jing. Bioinformatics analysis of gene expression profile in pediatric patients with chronic rhinosinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 171-180.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd