Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2022, Vol. 36 ›› Issue (3): 171-180.doi: 10.6040/j.issn.1673-3770.0.2021.556

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Bioinformatics analysis of gene expression profile in pediatric patients with chronic rhinosinusitis

LI Lin1, GAO Zhengwen2, CUI Nan3, SUN Jianping4, HUANG Xianming5, CUI Jing4   

  1. 1. Department of Otolaryngology, Qingdao Women and Children's Hospital, Qingdao 266011, Shandong, China;
    2. Department of Anesthesiology, Guzhen Orthopedic Hospital of Chengyang, Qingdao 266000, Shandong, China;
    3. Hospital Management Institute, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China;
    4. Qingdao Municipal Center for Disease Control and Prevention / Qingdao Institute of Preventive Medicine, Qingdao 266033, Shandong, China;
    5. Department of Surgery, Qingdao Haici Medical Group, Qingdao 266000, Shandong, China
  • Published:2022-06-15

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Abstract: Objective We performed a preliminary bioinformatics analysis of genes from pediatric patients with chronic rhinosinusitis(CRS)to provide a scientific basis for preventing and treating CRS. Methods Data of pediatric patients with CRS were downloaded from Gene Expression Omnibus(GEO)database. Differentially expressed genes(DEGs)were screened in sinus mucosa tissue from pediatric patients with CRS and normal children. DEGs associated with the Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway were analyzed using the DAVID tool and Gene Set Enrichment Analysis(GESA). Protein-protein interaction(PPI)network associated with DEGs were analyzed using STRING and Cytoscape. Results Ninety-two DEGs were screened using adjusted P<0.05 and∣log2 FC∣>2. Of these DEGs, 57 were up-regulated, and 35 were down-regulated. GO analysis showed that up-regulated DEGs were enriched in phagocytosis, β cell receptor signaling pathway, bacterial defense response, immune response, and the external side of the plasma membrane. KEGG pathway analysis showed that up-regulated DEGs were enriched in salivary secretion, and down-regulated DEGs were enriched in retinol metabolism, chemical carcinogenesis, and tyrosine metabolism. All 49 DEGs were involved in the PPI network, with 61 edges, an average protein node degree of 1.51, and a local clustering coefficient of 0.387. The PPI enrichment had a significant difference(P<0.001). The top 10 hub genes were ASPM, NCAPG, TPX2, MCM10, TOP2A, STATH, ADH1C, ADH6, CYP26A1, and UGT2A2. Except for STATH, the hub genes were all in Modules 1 and 2. Conclusion The CRS pathogenesis in pediatric patients is mediated by interleukins, inflammation, immune response, β cell receptor signaling pathway, salivary secretion, and bacterial defense response by hub genes. Further studies are required to explore the putative mechanisms in pediatric patients with CRS.

Key words: Chronic rhinosinusitis, Pediatric patients, Gene ontology analysis, KEGG analysis, Protein-protein interaction network

CLC Number: 

  • R765.4
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