Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2025, Vol. 39 ›› Issue (4): 151-160.doi: 10.6040/j.issn.1673-3770.0.2024.161

• Original Article • Previous Articles     Next Articles

Bioinformatic analysis of miRNA-mRNA network involved in thyroid function impairment by high-fat diet

DOU Tao1, DOU Naixin1, WANG Ru1, YANG Qian1, GUAN Qingbo1, WANG Lei2, YU Chunxiao1   

  1. 1. Key Laboratory of Endocrine Glucose & Lipids Metabolism and Brain Aging, Ministry of Education, Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Shandong Key Laboratory of Endocrinology and Lipid Metabolism, Jinan 250021, Shandong, China2. Department of Gastroenterology, Affiliated Central Hospital of Shandong First Medical University, Jinan 250021, Shandong, China
  • Online:2025-07-20 Published:2025-08-11

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Abstract: Objective To analyze the miRNA-mRNA regulatory network involved in high-fat diet-induced damage to thyroid function using bioinformatics methods, and to provide new targets for early intervention in lipid toxicity-induced thyroid dysfunction. Methods Rats were fed a high-fat diet for 8 weeks to establish a model of thyroid function damage, with a normal diet group as control. Agilent chips were used to detect thyroid miRNA and mRNA expression, and the “limma” package in RStudio was used to screen for differentially expressed miRNA and mRNA. The miRwalk was used to predict potential downstream target genes regulated by differentially expressed miRNA. The predicted target genes and differentially expressed mRNA were intersected using weishengxin websites to establish a differential miRNA-mRNA network. Gene ontology(GO)annotation and kyoto encyclopedia of genes and genomes(KEGG)pathway analysis of the intersected mRNA were performed using the Metascape website. protein-protein interaction(PPI)analysis was conducted using the String online platform, and the PPI network hub was visualized using Cytoscape,hub genes were selected using the CytoNCA plugin in Cytoscape. A potential miRNA-mRNA network affecting thyroid function due to high-fat diet was established based on the selected hub genes. Results 27 upregulated and 6 downregulated miRNAs, 775 upregulated and 543 downregulated mRNAs were screened, with 301 overlapping mRNAs between downregulated miRNA targets and upregulated mRNAs, and 278 overlapping mRNAs between upregulated miRNA targets and downregulated mRNAs, resulting in 491 and 777 miRNA-mRNA pairs, respectively. GO and KEGG analysis revealed that differential mRNAs were enriched in pathways related to thyroid hormone synthesis and cell proliferation. Further screening identified 10 hub genes including Src, Pebp1, Il1b, Plcg1, Igf1, etc. A key miRNA-mRNA regulatory network was established based on these hub genes, including regulatory pairs such as miR-3473/Src、miR-339-3p/Igf1、miR-674-5p/Igf1、miR-339-3p/Ntrk2、miR-99b-3p/Ntrk2. Conclusion The miRNA and mRNA, such as miR-3473、Igf1 and Ntrk2 may be involved in high-fat diet-induced damage to thyroid function.

Key words: Hypothyroidism, High-fat diet, miRNA-mRNA network

CLC Number: 

  • R581.2
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