JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (2): 48-51.doi: 10.6040/j.issn.1673-3770.0.2014.369

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Expression of TSLP and its relationship with the infiltrsting regulatory T cells in chronic rhinosinusitis

ZHU Hui-tao, CAI Xiao-lan, FENG Xin, QI Jun-jun, ZHANG Jin-ling, MA Yue, LI Xue-zhong   

  1. Department of Otolaryng, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-19 Online:2015-04-16 Published:2015-04-16
  • Contact: 李学忠。E-mail:lxzebyh@163.com E-mail:lxzebyh@163.com

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Abstract: Objective To determine the expression of thymic stromal lymphopoietin(TSLP)and regulatory T cells(CD4+CD25+Foxp3+Treg) in human chronic rhinosinusitis(CRS) and to analyze their pathogenesis in CRS. Methods The sinus mucosa from 25 patients with CRS was studied. Inferior nasal turbinate from 28 patients with nasal septum deviation served as the control. Their histological characteristics were analyzed by HE stain. The expressions of TSLP and Foxp3 mRNA were detected by Q-RT-PCR. Immunohistochemistry was used to detect CD4+CD25+Foxp3+Treg cells. The correlation of TSLP with the number of Tregs and the correlation of TSLP with Foxp3 mRNA were evaluated. Results The expressions of TSLP mRNA, Foxp3 mRNA and Treg cells were significant higher in CRS compared with the control group(P<0.05). There was positive correlation between TSLP and Foxp3 mRNA(r=0.977 3, P<0.000 1). At the same time, there was positive correlation between TSLP and the number of Tregs(r=0.864 6, P<0.0001). Conclusion TSLP could play an important role in CRS by inducing Treg cells.

Key words: Thymic stromal lymphopoietin, Infiltrasting regulatory T cells, Foxp3 gene, Chronic rhinosinusitis

CLC Number: 

  • R765.4
[1] Watanabe N, Wang Y H, Lee H K, et al. Hassall's corpuscles instruct dendritic cells to induce CD4+ CD25+ regulatory T cells in human thymus[J]. Nature, 2005, 436(7054):1181-1185.
[2] Fontenot J D, Gavin M A, Rudensky A Y. Foxp3 programs the development and function of CD4+ CD25+ regulatory T cells[J]. Nature Immunol, 2003, 4(4):330-336.
[3] Nagarkar D R, Poposki J A, Tan B K, et al. Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis[J]. J Allergy Clin Immunol, 2013, 132(3):593-600. e12.
[4] Umetsu D T, DeKruyff R H. The regulation of allergy and asthma[J]. Immunological Review, 2006, 212(1):238-255.
[5] Mills K H G, McGuirk P. Antigen-specific regulatory T cells-their induction and role in infection[C]//Seminars in immunology. Academic Press, 2004, 16(2):107-117.
[6] Walker M R, Carson B D, Nepom G T, et al. De novo generation of antigen-specific CD4+ CD25+ regulatory T cells from human CD4+ CD25-cells[J]. Proc Natl Acad Sci U S A, 2005, 102(11):4103-4108.
[7] von Boehmer H. Selection of the T-cell repertoire: receptor-controlled checkpoints in T-cell development[J]. Adv Immunol, 2004, 84(2):201-238.
[8] Josefowicz S Z, Rudensky A. Control of regulatory T cell lineage commitment and maintenance[J]. Immunity, 2009, 30(5):616-625.
[9] Kim Y M, Munoz A, Hwang P H, et al. Migration of regulatory T cells toward airway epithelial cells is impaired in chronic rhinosinusitis with nasal polyposis[J]. Clin Immunol, 2010, 137(1):111-121.
[10] Reche P A, Soumelis V, Gorman D M, et al. Human thymic stromal lymphopoietin preferentially stimulates myeloid cells[J]. J Immunol, 2001, 167(1):336-343.
[11] Caretto D, Katzman S D, Villarino A V, et al. Cutting edge: the Th1 response inhibits the generation of peripheral regulatory T cells[J]. J Immunol, 2010, 184(1):30-34.
[12] Zhou L, Chong M M W, Littman D R. Plasticity of CD4+ T cell lineage differentiation[J]. Immunity, 2009, 30(5):646-655.
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