JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (5): 110-114.doi: 10.6040/j.issn.1673-3770.0.2016.322

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Effect of silencing PDCD4 gene on proliferation of Hep-2 cells and the expression of β-catenin by RNA interference technique.

XU Yuanteng1, CHEN Ruiqing2, LIN Gongbiao1, FANG Xiuling1, YU Shujuan1, LIANG Xiaohua3, ZHANG Rong   

  1. 1. Department of Otolaryngology;2. Central Laboratory;3. Clinical Laboratory, The First Affliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian, China
  • Received:2016-07-20 Online:2016-10-20 Published:2016-10-20

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Abstract: Objective To study the effect of silencing PDCD4 gene on proliferation of Hep-2 cells and the expression of β-catenin by RNA interference technique. Methods Specific shRNA plasmid and negative control plasmid of PDCD4 gene were designed and synthesized. Hep-2 cells were transfected with shRNA plasmid and negative control plasmid. Real-time RT-PCR and Western blotting analysis were performed to determine the expressions of PDCD4 and β-catenin mRNA and protein after transfection, respectively. The proliferation of Hep-2 cells was observed by colony-forming assay. Results Compared to the control group, the colony formation rate of Hep-2 cells was significantly increased in the shRNNA plasmid group(P=0.01); the expressions of PDCD4 mRNA and protein were significantly reduced(P<0.01);the expressions of β-catenin mRNA and protein were significantly increased(P<0.01). Conclusion RNA interference technique can effectively silence PDCD4 gene expression of Hep-2 cells,enhance the colony forming ability of Hep-2 and increase the expressions of β-catenin mRNA and protein.

Key words: Hep-2 cell, PDCD4 gene, β-catenin, Cell proliferation, Laryngeal cancer, RNA interference

CLC Number: 

  • R739.65
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