JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (4): 64-67.doi: 10.6040/j.issn.1673-3770.0.2016.494

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RASSF2A methylation in laryngeal squamous cell carcinoma.

ZHANG Mingde1, ZHANG Zuping2, YU Xuemin3, WEI Yanhong1, YUAN Ying3   

  1. 1. Department of Otolaryngology, Liaocheng Dongchangfu Peoples Hospital, Liaocheng 252000, Shangdong, China;2. Department of Otolaryngology, Liaocheng Peoples Hospital, Liaocheng 252000, Shangdong, China;3. Department of Otolaryngology &Head and Neck Surgery, Qilu Hospital of Shandong University, Qingdao 266035, Shandong, China
  • Received:2016-11-23 Online:2017-08-16 Published:2017-08-16

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Abstract: Objective To evaluate the effects of methylation on RASSF2A expression during the development of laryngeal squamous cell carcinoma(LSCC). Methods Methylation-specific PCR was used to measure RASSF2A methylation and mRNA levels in tissues from 50 LSCCs(LSCC group)and 10 normal laryngeal tissues(control group). Results Hypermethylation was detected in the promoter region of RASSF2A in 54%(27/50)of the laryngeal cancer tissue samples but was not detected in control group tissues. The difference in hypermethylation was statistically significant(χ2=9.818, P=0.002). No significant correlations were found between the methylation levels of RASSF2A and the clinicopathological features of the patients with laryngeal cancer. The levels of RASSF2A mRNA in the LSCC group were significantly lower than those in normal mucosal tissues(χ2=9.818, P=0.002), because of methylation in RASSF2A in the laryngeal cancer tissue samples. Conclusion Epigenetic inactivation of RASSF2A through aberrant promoter methylation may play an important role during oncogenesis and the progression of LSCC.

Key words: Methylation, RASSF2A, Laryngeal squamous cell carcinoma

CLC Number: 

  • R739.65
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