Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (4): 31-35.doi: 10.6040/j.issn.1673-3770.1.2019.031

• Clinical Research • Previous Articles     Next Articles

Effects of dihydroartemisinin on endoreticulum stress pathway in FaDu hypopharyngeal carcinoma cells

Zelei HUANG1,Qi SONG2,Xiuying LU2,Lanzhen CUI2,Xiaoming LI2,*()   

  1. 1. Department of otolaryngology, Hebei General Hospital, Shijiazhuang 050057, Hebei, China
    2. Department of otolaryngology, Bethune International Peace Hospital, Shijiazhuang 050082, Hebei, China
  • Received:2019-05-13 Online:2019-07-16 Published:2019-07-22
  • Contact: Xiaoming LI E-mail:xmlmo@126.com

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Abstract: Objective

To investigate the mechanism of inhibition by dihydroartemisinin (DHA) of the proliferation of FaDu cells in hypopharyngeal cancer, and provide a theoretical basis for its clinical application in anti-tumor therapy.

Methods

FaDu cells were cultured in different concentrations of glucose (5.5, 10, 15, 25, 50, 100 mM) for 36 h, then the expression of p-STAT3 and endoplasmic reticulum stress-related protein GRP78 were detected by western blot. FaDu cells of hypopharyngeal carcinoma were treated with DHA at different concentrations (10, 20, 40, 80, 160 M) in a culture medium with 25 mM glucose, and the effect on cell proliferation was detected by MTT assay 24 h later. Western blot was used to detect the expression of p-STAT3 and GRP78.

Results

DHA inhibited the proliferation of FaDu hypopharyngeal carcinoma cells in a concentration-dependent manner. Low-glucose concentration medium reduced the expression of p-STAT3 and increased the expression of GRP78. DHA inhibited the expression of p-STAT3; GRP78 expression was stimulated at low DHA concentrations, but was inhibited as the DHA concentration increased.

Conclusions

DHA significantly inhibits the proliferation of FaDu hypopharyngeal carcinoma cells, inhibits the expression of p-STAT3, and induces endoplasmic reticulum stress, which may be closely related to the interference of cellular glucose metabolism.

Key words: Dihydroartemisinin, Hypopharyngeal cance, Fadu cells, Endoreticulum stress

CLC Number: 

  • R739.6

Fig.1

Effects of different concentrations of DHA on FaDu cells after 24 h"

Fig.2

Effects of different concentrations of glucose medium on the expression of p-STAT3, STAT3, and GRP78"

Fig.3

Effects of different concentrations of DHA on the expression of p-STAT3, STAT3, and GRP78"

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