JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (2): 67-72.doi: 10.6040/j.issn.1673-3770.0.2016.425

Previous Articles     Next Articles

Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma.

  

  1. 1.Department of Otorhinolaryngology, First Affiliated Hospital of Nanjing Medical University/Jiangsu Provincial Peoples Hospital, Nanjing 210029, Jiangsu, China;2.Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, China
  • Received:2016-10-09 Online:2017-04-16 Published:2017-04-16

PDF (PC)

356

Abstract: Objective To study the effect of curcumin in combination of resveratrol on proliferation in the head and neck neoplasm cell lines. Methods Hep2 cells and FaDu cells were treated with Curcumin and resveratrol. MTT was used to determine cell proliferation. Colony formation was used to detect the cell viability. Hoechst 33258 staining was performed to observe the cell apoptosis. The mRNA expressions of Bax and Bcl-2 were analyzed by Real-time PCR. Results Hep2 and FaDu cell proliferation could be inhibited with curcumin and resveratrol in a dose-dependent manner. Their combination exhibited the best inhibitory effects. Curcumin combined with resveratrol exhibited colony repression and showed a more significant apoptosis in Hep2 and FaDu cells. Real-time PCR demonstrated that Bax mRNA was upregulated and Bcl-2 mRNA was downregulated by curcumin and resveratrol. Meanwhile, Bax mRNA was upregulated and Bcl-2 mRNA was downregulated by curcumin and resveratrol. Conclusion The combination of curcumin and resveratrol can inhibit the proliferation of Hep2 and FaDu cells more significantly. The potential mechanism may be related with the up-regulation of Bax and the down-regulation of Bcl-2.

Key words: Proliferation, Curcumin, Resveratrol, Hep2 cells, FaDu cells, Apoptosis

CLC Number: 

  • R739.91
[1] Parkin DM, Pisani P, Ferlay J. Global cancer statistics[J]. CA Cancer Clin, 1999, 49(1):33-64.
[2] Kamangar F, Dores GM, Anderson WF, Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world[J]. J Clin Oncol, 2006, 24(14):2137-2150.
[3] Pezzuto F, Buonaguro L, Caponigro F, et al. Update on head and neck cancer: current knowledge on epidemiology, risk factors, molecular features and novel therapies[J]. Oncol, 2015, 89(1):125-136.
[4] 李子强, 白晓光, 杨志衡, 等. 姜黄素类化合物与生物活性研究进展[J]. 中国医药工业杂志, 2015, 46(3):305-311.
[5] 刘晓真, 张宏颖.姜黄素与肿瘤细胞作用的分子机制[J]. 大连医科大学学报, 2008, 30(5):465-468. LIU Xiaozhen, ZHANG Hongying. Molecularmechanism ofeffectofcurcuminontumor[J]. J Dalian Med Univ, 2008, 30(5):465-468.
[6] 黄珈雯.姜黄素的抗肿瘤作用研究进展[J]. 甘肃中医, 2008, 21(11):55-56.
[7] Fulda S, Debatin KM.Sensitization for tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by the chemopreventive agent resveratrol[J].Cancer Res, 2004,64(1):337-346.
[8] Proviciali M, Re F, Donnin A.Effect of resveratrol on the development of spontaneous mammary tumors in HER-2/neu transgenic mice[J].Int J Cancer, 2005, 115(1):36-45.
[9] Leemans CR, Braakhuis BJ, Rrakenhoff RH, The molecular biology of head and neck cancer[J]. Nat Rev Cancer, 2011, 11(1):9-22.
[10] Borges GA, Rego DF, Assad DX, et al. In vivo and in vitro effects of curcumin on head and neck carcinoma: a systematic review[J]. J Oral Pathol Med, 2017, 46(1):3-20.
[11] Hu A, Huang JJ, Li RL, et al. Curcumin as therapeutics for the treatment of head and neck squamous cell carcinoma by activating SIRT1[J]. Sci Rep, 2015, 5:13429.
[12] Tyagi A, Gu M, Takahata T, et al. Resveratrol selectively induces DNA Damage, independent of Smad4 expression, in its efficacy against human head and neck squamous cell carcinoma[J]. Clin Cancer Res, 2011. 17(16):5402-5411.
[13] Piao L, Mukherjee S, Chang Q, et al. TriCurin, a novel formulation of curcumin, epicatechin gallate, and resveratrol, inhibits the tumorigenicity of human papillomavirus-positive head and neck squamous cell carcinoma[J]. Oncotarget, 2016,DOI: 10.18632/oncotarget.10620
[14] Dandawate PR, Subramaniam D, Jensen RA, et al.Targeting cancer stem cells and signaling pathways by phytochemicals: Novel approach for breastcancer therapy[J]. Semin Cancer Biol, 2016, 41:192-208.
[15] Ryu MJ, Cho M, Song JY, et al. Natural derivatives of curcumin attenuate the Wnt/beta-catenin pathway through down-regulation of the transcriptional coactivator p300[J]. Biochem Biophys Res Commun, 2008, 377:1304-1308.
[16] Atten MJ, Godoy-Romero E, Attar BM, et al. Resveratrol regulates cellular PKC alpha and delta to inhibit growth and induce apoptosis in gastric cancer cells[J]. Invest New Drugs, 2005, 23:111-119.
[17] Nonn L, Duong D, Peehl DM, et al. Chemopreventive anti-inflammatory activities of curcumin and other phytochemicals mediated by MAP kinase phosphatase-5 in prostate cells[J]. Carcinogenesis, 2007, 28(6):1188-1196.
[18] Du Q, Hu B, An HM, et al. Synergistic anticancer effects of curcumin and resveratrol in Hepa1-6 hepatocellular carcinoma cells[J]. Oncol Rep, 2013, 29(5):1851-1858.
[19] Suzuki O, Abe M. Cell surface N-glycosylation and sialylation regulate galectin-3-induced apoptosis in human diffuse large B cell lymphoma [J]. Oncol Rep, 2008, 19(3):743-748.
[20] Takai N, Ueda T, Nishida M.Histone deacetylase inhibitors induce growth inhibition, cell cycle arrest and apoptosis in human choriocarcinoma cells [J].Int J Mol Med, 2008, 21(1):109-115.
[21] Masuelli L, Di Stefano E, Fantini M. Resveratrol potentiates the in vitro and in vivo anti-tumoral effects of curcumin in head and neck carcinomas[J]. Oncotarget, 2014, 5(21):10745-10762.
[22] Kiraz Y, Adan A, Kartal Yandim M. Major apoptotic mechanisms and genes involved in apoptosis[J]. Tumour Biol, 2016: 10.1007/s13277-016-5035-9
[23] Kumar S. Caspase function in programmed cell death[J]. Cell Death Differ, 2006, 14(1):32-43.
[24] 赵永强,冯慧伟,范献良,等.塞来昔布诱导喉癌Hep-2细胞凋亡的实验研究 [J]. 山东大学耳鼻喉眼学报, 2014,28(2):41-45. ZHAO Yongqiang, FENG Huiwei, FAN Xianliang, et al. Apoptosis of Hep-2 cells induced by celecoxib[J]. J Otolaryngol Ophthalmol Shandong Univ, 2014, 28(2):41-45.
[1] SU Jie, AI Xin, MA Chunmei, YANG Fuyu, HUANG Shuai. Relationship between acute intraocular hypertension and lateral geniculate neuron autophagy and apoptosis in rats [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 68-71.
[2] LI Guobin, ZHANG Zhancheng, WANG Xinyan. Up-regulation of miR-200b may inhibit epithelial mesenchymal transition process to prevent the proliferation, migration, and invasion of human laryngeal cancer Hep-2 cells [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 53-57.
[3] ZHAO Xiaoyan, WU Caiqin, REN Xiaoyong, WANG Zhenghui. A polysaccharide from hedyotis diffusa induces apoptosis and inhibits migration in Hep-2 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(2): 84-87.
[4] ZHANG Caixia, LIU Yangyun, JIANG Wen, LIU Gengxun, CAO Hang, CHEN Qiong, ZHANG Jishuai. Investigation of chemosensitization induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 72-78.
[5] ZHOU Yibo, GONG Xiaorong, YU Feng. Effects of miR-150 targeted to Nanog on proliferation and invasion of nasopharyngeal carcinoma side population cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 79-84.
[6] XU Yuanteng, CHEN Ruiqing, LIN Gongbiao, FANG Xiuling, YU Shujuan, LIANG Xiaohua, ZHANG Rong. Effect of silencing PDCD4 gene on proliferation of Hep-2 cells and the expression of β-catenin by RNA interference technique. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(5): 110-114.
[7] LI Qin, CHEN Yanlin, MA Yanyi, ZHANG Yongdong. Effects of interferon gamma on Eosinophil apoptosis and Eotaxin levels in nasal lavage fluid of allergic rhinitis rats. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 30-33.
[8] CHEN Xiaoling, FEI Bing, LI Xianbin, WU Hao. Construction and identification of small interfering RNA targeting silence Trop2 expression in human laryngeal carcinoma cell line HEP2 [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(3): 51-53.
[9] CHEN Chunli, SONG Zongming, JIA Xinguo, ZHOU Zhonglou, WANG Zhaoyang. Erythropoietin inhibits Müller cell apoptosis induced by oxidative damage [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(3): 65-71.
[10] QIN Jie-sheng1, WANG Hui-ge1, LIN Xin-qiang1, HONG Liang-li2. Expression and significance of IL-15mRNA in nasal polyps [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 34-37.
[11] ZHUANG Xiao-yan1,2, LI Qin2, CHEN Yan-lin2, MA Yan-yi2. Effects of budesonide on eosinophil apoptosis in nasal lavage fluid of allergic rhinitis rats [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 68-71.
[12] ZHAO Yong-qiang1, FENG Hui-wei1, YU Ke-na1, SHI Mei2, FAN Xian-liang1. Apoptosis  of Hep-2 cells induced  by celecoxib [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 41-45.
[13] WEI Xi-jian1, YIN Rui-lan2, GUO Xing3. Expression of a proliferation-inducing ligand in laryngeal squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(1): 14-16.
[14] ZHANG Shu-jia1, LI Pei-hua2, JIA Wen-bo1, XU Heng-guang1, BAO Feng-xiang1, LI Li1. 1. Expression and clinical significance of programmed cell death 5 (PDCD5) in laryngeal squamous cell carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 23-26.
[15] TIAN Tian-jie1， GENG Yang1， YE Qing1,2， ZHANG Xian-zeng3， XIE Shu-sen3， SHEN Fan1. Apoptosis and the injury of the inner ear [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 35-38.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd