Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (4): 138-144.doi: 10.6040/j.issn.1673-3770.0.2018.594

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Neuroprotective effects and safety of stem cell transplantation in rats with experimental glaucoma: a systematic review

LIU Lin1, ZHENG Hua2, CHEN Shaolin1, DUAN Xuanchu2   

  1. Changsha Aier Eye Hospital, Changsha 41000, Hunan, China
  • Online:2019-07-20 Published:2019-07-22

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Abstract: Objective To systematically review the neuroprotective effects and safety of transplanted stem cells in rats with experimental glaucoma. Methods Databases including PubMed, Embase, the Cochrane Library, Ovid, CBM, CNKI, VIP, and Wanfang Data were searched to collect information on animal experiments of stem cell transplantation for glaucoma, from inception to June 10, 2018. Two reviewers independently screened the literature in accordance with the inclusion and exclusion criteria, extracted the data, and assessed the risk of bias of the included studies using SYRCLE's risk of bias tool. Subsequently, a meta-analysis was performed using the RevMan 5.3 software. Results A total of 8 studies involving 319 rats were analyzed. The results of the meta-analysis showed that: 1)on the 28th day, the intraocular pressure was lower in the experimental group that in the control group ［SMD=-2.12, 95% CI:(-3.39, -0.85), Z=3.27, P=0.001］; 2)on the 14th day and the 28th day, the retinal ganglion cells were more in the experimental group than in the control group ［SMD=4.51, 95% CI:(3.17, 5.85), Z=6.60, P<0.001; SMD=2.96, 95% CI:(1.11, 4.80), Z=3.14, P=0.002］; 3)on the 14th day, the expression of BDNF was more in the retina of the experimental group than in the control group ［SMD=2.67, 95% CI:(1.46, 3.88), Z=4.32, P<0.001］; 4)on the 14th day and the 28th day, the expression of GDNF was more in the retina of the experimental group than in the control group ［WMD=1.21, 95% CI:(1.05, 1.37), Z=14.75, P<0.001; WMD=0.79, 95% CI:(0.52, 1.06), Z=5.72, P<0.001］; 5)on the 14th day, the expression of IGF-I was more in the retina of the experimental group than in the control group ［SMD=4.31, 95% CI:(2.51, 6.11), Z=4.69, P<0.001］. Three studies showed that stem cells could survive in the eye for 4 weeks and a few cells could integrate in the host retina and express neural cell surface markers. Three studies showed no adverse reactions after transplantation. Conclusions Although the current literature shows no uniform, standardized treatment method for experimental glaucoma in rats, there is evidence that stem cell transplantation has a certain protective effect on the optic nerve of rats with glaucoma, with no adverse reactions after transplantation. Because of the limited quality and quantity of the included literature, further studies are needed to validate the results of this review.

Key words: A rat model of glaucoma, Stem cells, Randomized controlled trial, Meta-analysis, Systematic review

CLC Number: 

  • R775.9
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