Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2020, Vol. 34 ›› Issue (1): 78-84.doi: 10.6040/j.issn.1673-3770.0.2019.555

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Expression of the alternative splice forms of Col2a1 in mice eyes, ears and cartilage at various development stages

HAO Peixia1, LIU Xiuzheng2, ZHANG Xiaolin1, FAN Yiyan1, DONG Hongliang2, REN Jizhen1, YIN Jingjing1, MA Xiufang1   

  1. 1. Department of Otolaryngology, the Affiliated Binzhou Medical University Hospital, Binzhou 256600, Shandong, China;
    2. Clinical Medicine Laboratory, Binzhou Medical University Hospital, Binzhou 256600, Shandong, China
  • Published:2020-03-06

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Abstract: Objective To study the expression of the alternative splice forms of Col2a1 in mice eyes, ears and cartilage at various development stages and lay a foundation for further understanding the role of Col2a1 in the development and function of these tissues and organs. Methods Obtained eye, ear and cartilage tissues of mice embryos E12.5, E16.5 and postnatal D0, D7, D14, D21, D28, then analyzed the mRNA expression level of the splice forms: IIA, IIB,IIC and IID of Col2a1 through real-time PCR. Results Four splice forms of Col2a1 were detected in three tissues; expression levels of IIA and IIB were higher than IIC and IID, and IIC was the lowest. All splice forms were highiy expressed in eyes and ears at embryonic stages, then decreased at postnatal to adult stages, and splice forms except IIB presented the same trends in cartilage. Expression of IIB was low in the three tissues at the embryonic stage, then its expression increased with development. The ratio of IIA to IIB varied dramatically in cartilage, it was no more than 2 at E12.5, and decreased to 0.004 at D28; while the ratio in eyes and ears also appeared to decline from embryo to adult stages, but was still above 1 at the adult stage. Conclusion Expression of the four splice forms of Col2a1 in eye, ear and cartilage tissue were all regulated by development stages with IIA and IIB being the main forms. IIA expressed actively in all three tissues at embryonic stages, and was replaced by IIB in adult cartilage, while IIA remained higher than IIB in adult eyes and ears.

Key words: Col2a1, Alternative splice form, Development stage, Eye, Ear, Cartilage

CLC Number: 

  • R76
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