ISSN 1673-3770 CN 37-1437/R
山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (3): 16-19.doi: 10.6040/j.issn.1673-3770.0.2015.087
门永芝, 程宇晟, 李煜, 董频, 於子卫
MEN Yongzhi, CHENG Yusheng, LI Yu, DONG Pin, YU Ziwei
摘要： 目的 研究雪旺细胞(SCs)、神经干细胞(NSCs)与内置纵行排列纳米纤维丝的静电纺丝壳聚糖-聚羟基乙酸 (Chitosan-PLGA)纳米导管材料的细胞相容性, 探讨细胞在导管材料上的黏附及定向生长情况。方法 实验分为SCs组、NSCs组、SCs-NSCs共培养组, 3组细胞分别与Chitosan-PLGA纳米导管材料复合培养, 四甲基偶氮唑蓝(MTT)比色法观察各组细胞存活率, 扫描电镜下观察SCs、NSCs在Chitosan-PLGA上的生长。结果 细胞存活率排序由高至低依次为共培养细胞组、SCs组、NSCs组。SCs及NSCs能贴附在Chitosan-PLGA 内置纳米纤维丝上生长并沿纤维丝定向生长。结论 雪旺细胞及神经干细胞共培养可促进细胞与Chitosan-PLGA材料的相容性。内置纵行排列的纳米纤维丝有引导细胞定向生长与迁移的作用。
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| [1] Rezwan K, Chen Q Z, Blaker J J, et al. Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering[J]. Biomaterials, 2006, 27(18):3413-3431. [2] Yang J, Shi G, Bei J, et al. Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture[J]. J Biomed Mater Res, 2002, 62(3):438-446. [3] Chen Y S, Hsieh C L, Tsai C C, et al. Peripheral nerve regeneration using silicone rubber chambers filled with collagen, laminin and fibronectin[J]. Biomaterials, 2000, 21(15):1541-1547. [4] Sorenson E J, Windebank A J. Relative importance of basement membrane and soluble growth factors in delayed and immediate regeneration of rat sciatic nerve[J]. J Neuropathol Exp Neurol, 1993, 52(3):216-222. [5] Schmalenberg K E, Uhrich K E. Micropatterned polymer substrates control alignment of proliferating Schwann cells to direct neuronal regeneration[J]. Biomaterials, 2005, 26(12):1423-1430. [6] Rodriguez A M, Pisani D, Dechesne C A, et al. Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse[J]. J Exp Med, 2005, 201(9):1397-1405. [7] Gardner R L. Stem cells and regenerative medicine: principles, prospects and problems[J]. C R Biol, 2007, 330(6-7):465-473. [8] 沈华, 沈尊理, 陈南樑, 等.不同浓度壳聚糖涂层PLGA材料与许旺细胞相容性研究[J].中国美容医学, 2009, 18(11):1627- 1631. SHEN Hua, SHEN Zunli, CHEN Nanliang, et al. The cytocompatibility of PLGA scaffolds coated with different concentration of chitosan with schwann cells in vitro[J]. Chin J Aesth Med, 2009, 18(11):1627- 1631. [9] Alessandri G, Emanueli C, Madeddu P. Genetically engineered stem cell therapy for tissue regeneration[J]. Ann N Y Acad Sci, 2004, 1015:271-284. [10] Goldman S A, Sim F. Neural progenitor cells of the adult brain[J]. Novartis Found Symp, 2005, 265:p. 66-80; discussion 82-97. [11] Xia, L, Wan H, Hao S Y , et al. Co-transplantation of neural stem cells and Schwann cells within poly (L-lactic-co-glycolic acid) scaffolds facilitates axonal regeneration in hemisected rat spinal cord[J]. Chin Med J, 2013, 126(5):909-917. [12] Zhang X, Zeng Y, Zhang W, et al. Co-transplantation of neural stem cells and NT-3-overexpressing Schwann cells in transected spinal cord[J]. J Neurotrauma, 2007, 24(12):1863-1877. [13] Zeng Y S, Ding Y, Wu L Z, et al. Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord[J]. Dev Neurosci, 2005, 27(1):20-26. [14] Xu L, Zhou S, Feng G Y, et al. Neural stem cells enhance nerve regeneration after sciatic nerve injury in rats[J]. Mol Neurobiol, 2012, 46(2):265-274. |
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