JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2018, Vol. 32 ›› Issue (2): 84-87.doi: 10.6040/j.issn.1673-3770.0.2017.323

Previous Articles     Next Articles

A polysaccharide from hedyotis diffusa induces apoptosis and inhibits migration in Hep-2 cells

ZHAO Xiaoyan, WU Caiqin, REN Xiaoyong, WANG Zhenghui   

  1. Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital, Xian Jiaotong University, Xian 710004, Shaanxi, China
  • Received:2017-07-28 Online:2018-03-20 Published:2018-03-20

PDF (PC)

738

Abstract: Objective The aim of this study was to explore the anti-tumor potential of a polysaccharide isolated from Hedyotis diffusa(HDP)in Hep-2 human larynx squamous carcinoma cells. Methods Cell cycle and apoptosis were analyzed by detecting changes in protein expression by flow cytometry. Apoptosis was further characterized by detecting relevant proteins by western blotting. Results HDP suppressed the proliferation of Hep-2 cells in a time- and dose-dependent manner. Cell cycle analysis revealed that exposure to HDP(400 μg/mL)caused G0/G1 cell cycle arrest in Hep-2 cells. Moreover, flow cytometry revealed that Hep-2 cells treated with HDP for 24 h underwent apoptosis to a significantly greater extent than untreated control cells. HDP treatment promoted the expression of caspase-3, caspase-8, and caspase-9, and reduced the expression of Bcl-2. HDP also inhibited cell migration and suppressed the expression of MMP-2 and uPA. Conclusion HDP possesses suppressive effects against aggressive phenotypes of human larynx squamous carcinoma cells and may have therapeutic potential for this particular malignancy.

Key words: Hedyotis diffusa, Polysaccharide, Hep-2 Cells, Apoptosis, Migration

CLC Number: 

  • R739.6
[1] Hu E, Wang D, Chen J, et al. Novel cyclotides from hedyotis diffusa induce apoptosis and inhibit proliferation and migration of prostate cancer cells[J]. Int J Clin Exp Med, 2015, 8(3):4059-4065.
[2] Lin JM, Wei LH, Xu W, et al. Effect of hedyotis diffusa willd extract on tumor angiogenesis[J]. Mol Med Rep, 2011, 4(6):1283-1288.
[3] 苏芳, 魏菁, 何龙. 白花蛇舌草对人喉癌细胞凋亡及增殖的影响[J]. 实用中医内科杂志, 2012, 26(7):1-2. SU Fang, WEI Jing, HE Long. The effect of herba hedyotis diffusae on apoptosis and proliferation of human laryngeal carcinoma cells[J]. J Prac Trad Chin Int Med, 2012, 26(7):1-2.
[4] 张军, 张耀明, 王正辉, 等. 草苁蓉多糖提取物诱导人喉癌Hep2细胞凋亡的实验研究[J]. 陕西医学杂志, 2014, 43(8):947-949. ZHANG Jun, ZHANG Yaoming, WANG Zhenghui, et al. Polysaccharide of boschniakia rossica induces apoptosis on Hep2 cells[J]. Shaanxi Med J, 2014, 43(8):947-949.
[5] 张健梅, 文姝, 李洁, 等. 三氧化二砷与紫杉醇联合诱导Hep-2细胞凋亡的体外研究[J]. 中国耳鼻咽喉头颈外科, 2011, 18(4):196-199. ZHANG Jianmei, WEN Shu, LI Jie, et al. Study in the effect of arsenic trioxide and taxol on Hep-2 in vitro[J]. Chin Arch Otolaryngol Head Neck Surg, 2011, 18(4):196-199.
[6] Wang SY, Hsu ML, Hsu HC, et al. The anti-tumor effect of ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes[J]. Int J Cancer, 1997, 70(6):699-705.
[7] Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides[J]. Appl Microbiol Biotechnol, 2002, 60(3):258-274.
[8] Zhang P, Zhang B, Gu J, et al. The study of the effect of hedyotis diffusa on the proliferation and the apoptosis of the cervical tumor in nude mouse model[J]. Cell Biochem Biophys, 2015, 72(3):783-789.
[9] Zhang Y, Xie RF, Xiao QG, et al. Hedyotis diffusa willd extract inhibits the growth of human glioblastoma cells by inducing mitochondrial apoptosis via AKT/ERK pathways[J]. J Ethnopharmacol, 2014, 158:404-411.
[10] Lu QL, Poulsom R, Wong L, et al. Bcl-2 expression in adult and embryonic non-haematopoietic tissues[J]. J Pathol, 1993, 169(4):431-437.
[11] Cui M, Zhang M, Liu HF, et al. Effects of microRNA-21 targeting PITX2 on proliferation and apoptosis of pituitary tumor cells[J]. Eur Rev Med Pharmacol Sci, 2017, 21(13):2995-3004.
[12] Garner TP, Lopez A, Reyna DE, et al. Progress in targeting the BCL-2 family of proteins[J]. Curr Opin Chem Biol, 2017, 39:133-142.
[13] 孙翔宇, 何丽娜, 吕克文, 等. 三氧化二砷对人舌鳞癌细胞CAL-27侵袭能力的影响[J]. 国际口腔医学杂志, 2014, 41(5):541-545. SUN Xiangyu, HE Lina, LÜ Kewen, et al. The effect of arsenic trioxide on invasion of human tongue squamous carcinoma cell line CAL-27[J]. Inter J Stom, 2014, 41(5):541-545.
[14] Kawata R, Shimada T, Maruyama S, et al. Enhanced production of matrix metalloproteinase-2 in human head and neck carcinomas is correlated with lymph node metastasis[J]. Acta Otolaryngol, 2002, 122(1):101-106.
[15] 刘斐斐, 李薇, 尚伟, 等. 喉鳞癌组织MMP-2和uPA表达与肿瘤侵袭转移关系[J]. 青岛大学医学院学报, 2008, 44(3):238-240. LIU Feifei, LI Wei, SHANG Wei, et al. Expressions of MMP-2 and uPA in laryngeal squamous cell carcinoma and their relationship with lymph node metastasis[J]. Act Acad Med Qingdao Univ, 2008, 44(3):238-240.
[1] WU Min, LI Zhengyang, MENG Jie, YE Huiping. Molecular mechanisms of programmed cell death and its role in nasopharyngeal carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 152-157.
[2] FU Yixuan, WANG Tao, CAO Jing, LI Guangda, ZHENG Lingfang, XU Wanjing, ZHAO Shuang. The protective effect of hydroxysafflor yellow A on light-induced retinal injury in rats [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 128-134.
[3] XU Ling, SUN Yan. Advances in research on the trifoliate factor family in the middle ear respiratory mucosal epithelium [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(1): 93-98.
[4] ZOU Liangyu, LI Lianhe, YUE Wenhui, HAN Zhipeng, ZHANG Zihui. Clinical significance and correlation between MIF and GSK-3β expression in laryngeal squamous cell carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 76-80.
[5] XIE Yi, HAN Fengchan. Role of miRNAs in inner ear development and apoptosis or regeneration of auditory hair cells [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 126-129.
[6] XU Li, WANG Zhiyi, CHEN Wei, XUE Fei, ZHANG Yong, CHENG You, JI Junfeng, CUI Pengcheng, JIANG Manjie. Effect of MicroRNA-24 on the proliferation, migration, invasion, and apoptosis of LSCC cells [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2018, 32(6): 30-37.
[7] SU Jie, AI Xin, MA Chunmei, YANG Fuyu, HUANG Shuai. Relationship between acute intraocular hypertension and lateral geniculate neuron autophagy and apoptosis in rats [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 68-71.
[8] ZHANG Caixia, LIU Yangyun, JIANG Wen, LIU Gengxun, CAO Hang, CHEN Qiong, ZHANG Jishuai. Investigation of chemosensitization induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 72-78.
[9] . Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(2): 67-72.
[10] LI Qin, CHEN Yanlin, MA Yanyi, ZHANG Yongdong. Effects of interferon gamma on Eosinophil apoptosis and Eotaxin levels in nasal lavage fluid of allergic rhinitis rats. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 30-33.
[11] QIN Jie-sheng1, WANG Hui-ge1, LIN Xin-qiang1, HONG Liang-li2. Expression and significance of IL-15mRNA in nasal polyps [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 34-37.
[12] ZHAO Yong-qiang1, FENG Hui-wei1, YU Ke-na1, SHI Mei2, FAN Xian-liang1. Apoptosis  of Hep-2 cells induced  by celecoxib [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 41-45.
[13] ZHUANG Xiao-yan1,2, LI Qin2, CHEN Yan-lin2, MA Yan-yi2. Effects of budesonide on eosinophil apoptosis in nasal lavage fluid of allergic rhinitis rats [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 68-71.
[14] ZHANG Shu-jia1, LI Pei-hua2, JIA Wen-bo1, XU Heng-guang1, BAO Feng-xiang1, LI Li1. 1. Expression and clinical significance of programmed cell death 5 (PDCD5) in laryngeal squamous cell carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 23-26.
[15] TIAN Tian-jie1， GENG Yang1， YE Qing1,2， ZHANG Xian-zeng3， XIE Shu-sen3， SHEN Fan1. Apoptosis and the injury of the inner ear [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 35-38.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd