Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (2): 111-114.doi: 10.6040/j.issn.1673-3770.0.2017.442

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Effect of beta elemene on the expression of IL-1 beta and ICAM-1 in the retina of diabetic rats

LI Baohua, LIU Ping, WANG Xin   

  1. Department of Ophthalmology, Zhengzhou Peoples Hospital, Zhengzhou 450003, Henan, China
  • Published:2019-03-28

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Abstract: Objective To study the effect of beta elemene on IL-1 beta and intercellular adhesion molecule 1(ICAM-1)expression levels in rats with diabetic retinopathy, to validate the potential of beta elemene in the prevention and treatment of diabetic retinopathy. Methods Forty adult SD healthy rats were randomly divided into four groups: normal control group, diabetic group, 40 mg/kg beta elemene treatment group, and 80 mg/kg beta elemene treatment group. Diabetes was induced in rats by intraperitoneal injection of streptozotocin(STZ). The rats in the beta elemene treatment group were treated with beta elemene solution for 12 weeks. They were then executed, and their retinal tissue, aqueous humor, and serum were extracted. ELISA was used to determine the IL-1 beta and ICAM-1 protein expression in retina, aqueous humor, and serum of rats. Immunohistochemistry was used to detect the expression levels of IL-1 beta and ICAM-1 proteins in retinal tissues of rats. Western blotting was done to evaluate the expression levels of IL-1 beta and ICAM-1 proteins in the retinal tissue. Results ELISA assay revealed that, compared with the normal control group, the levels of IL-1 beta and ICAM-1 proteins in diabetic rat serum and retina were significantly higher(P<0.05); however, 40 mg/kg treatment group and 80 mg/kg treatment exhibited significantly lower IL-1 beta and ICAM-1 levels in rat serum and retina compared to the diabetic group (P<0.05), among which 80 mg/kg beta elemene treatment group exhibited lower serum and retinal IL-1 beta and ICAM-1 protein levels compared to 40 mg/kg beta elemene treatment group (P<0.05). There was no significant difference in IL-1 beta and ICAM-1 protein levels in aqueous humor among the groups (P>0.05). Immunohistochemistry results showed that compared with the normal control group, retinal IL-1 beta protein levels were higher in the diabetic group; however, 40 mg/kg treatment group and 80 mg/kg treatment group exhibited lower IL-1 beta levels compared with diabetic group; the retinal tissue isolated from normal group exhibited almost no expression of ICAM-1 protein, whereas the diabetic group exhibited high expression of ICAM-1 protein. The 40 mg/kg treatment group and the 80 mg/kg treatment group exhibited lower expression levels of ICAM-1 compared to diabetic group. Western blotting revealed that, compared with the normal control group, diabetes group exhibited significantly higher expression levels of retinal IL-1 beta and ICAM-1 proteins (P<0.05); 40 mg/kg treatment group and 80 mg/kg treatment group exhibited significantly lower retinal IL-1 beta and ICAM-1 protein levels compared to diabetic group (P<0.05), among which 80 mg/kg treatment group exhibited significantly lower retinal IL-1 beta and ICAM-1 protein levels compared to 40 mg/kg treatment group (P<0.05). Conclusion Beta elemene can inhibit the production of IL-1 beta and ICAM-1 inflammatory factors in serum and retinal tissue of diabetic rats, suggesting that beta elemene may be used in the prevention and treatment of diabetic retinopathy.

Key words: Beta elemene, Diabetic retinopathy, IL-1 beta, Intercellular adhesion molecule 1

CLC Number: 

  • R774
[1] 张冬艳, 马鸿雁, 苏军燕, 等. 血清结缔组织生长因子、25-羟维生素D、脂联素检测在2型糖尿病视网膜病变诊断及病情评价中的价值[J]. 中国实验诊断学, 2017, 21(3):505-508. doi: 10.3969/j.issn.1007-4287.2017.03.044.
[2] 金慧昳, 刘堃, 许迅. 炎症、抗炎药物与早期糖尿病视网膜病变的关系[J]. 中华眼底病杂志, 2008, 24(4):312-315.
[3] McLaughlin F, Hayes BP, Horgan CM, et al. Tumor necrosis factor(TNF)-alpha and interleukin(IL)-1beta down-regulate intercellular adhesion molecule(ICAM)-2 expression on the endothelium[J]. Cell Adhes Commun, 1998, 6(5): 381-400.
[4] Lee SJ, Drabik K, Van Wagoner NJ, et al. ICAM-1-induced expression of proinflammatory cytokines in astrocytes: involvement of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways[J]. J Immunol, 2000, 165(8):4658-4666.
[5] Yang CR, Shih KS, Liou JP, et al. Denbinobin upregulates miR-146a expression and attenuates IL-1β-induced upregulation of ICAM-1 and VCAM-1 expressions in osteoarthritis fibroblast-like synoviocytes[J]. J Mol Med, 2014, 92(11): 1147-1158. doi: 10.1007/s00109-014-1192-8.
[6] 李雪, 周赟, 陈蕾. β-榄香烯主要抗肿瘤机制及在非肿瘤性疾病中的研究进展[J]. 安徽医药, 2015(8):1429-1432. doi:10.3969/j.issn.1009-6469.2015.08.001. LI Xue, ZHOU Yun, CHEN Lei. Research progress on the main anti-tumor mechanisms of β-elemene and its applications in non-oncologic diseases[J]. Anhui Med Pharmaceut J, 2015(8): 1429-1432. doi: 10.3969/j.issn.1009-6469.2015.08.001.
[7] 周赟, 刘宁宁, 万超, 等. β-榄香烯对糖尿病大鼠视网膜中肿瘤坏死因子-α表达的影响[J]. 眼科新进展, 2015, 35(12): 1129-1131. doi: 10.13389/j.cnki.rao.2015.0309. ZHOU Yun, LIU Ning-Ning, WAN Chao, et al. Effects of β-elemene on expression of TNF-α in retina of diabetic rats[J]. Rec Adv Ophthalmol, 2015, 35(12): 1129-1131. doi: 10.13389/j.cnki.rao.2015.0309.
[8] Shen X, Xu GZ. Role of IL-1β on the glutamine synthetase in retinal müller cells under high glucose conditions[J]. Current Eye Research, 2009, 34(9): 727-736. doi: 10.1080/02713680903030875.
[9] Nentwich MM, Ulbig MW. Diabetic retinopathy - ocular complications of diabetes mellitus[J]. World J Diabetes, 2015, 6(3): 489-499. doi: 10.4239/wjd.v6.i3.489.
[10] Matsunaga DR, Yi JJ, De Koo LO, et al. Optical coherence tomography angiography of diabetic retinopathy in human subjects[J]. Ophthalmic Surg Lasers Imaging Retina, 2015, 46(8): 796-805. doi:10.3928/23258160-20150909-03.
[11] 韩青. 糖尿病视网膜病变患者外周血IL-1β和TNF-α的检测及临床意义[J]. 中国实验诊断学, 2016, 20(4): 583-586. HAN Qing. Expression and clinical significance of serum IL-1βand TNF-αin patients with diabetic retinopathy[J]. Chin J Lab Diagn, 2016, 20(4): 583-586.
[12] 徐家窈, 冼文光, 刘俐娜. 血清VEGF及ICAM-1水平与糖尿病视网膜病变患者微血管损伤的关系[J]. 中国老年学杂志, 2017, 37(8): 2000-2002. doi: 10.3969/j.issn.1005-9202.2017.08.080.
[13] Veldhuis WB, Derksen JW, Floris S, et al. Interferon-beta blocks infiltration of inflammatory cells and reduces infarct volume after ischemic stroke in the rat[J]. J Cereb Blood Flow Metab, 2003, 23(9): 1029-1039. doi: 10.1097/01.WCB.0000080703.47016.B6.
[14] 郭建忠, 魏尧, 谷如雷, 等. β-榄香烯诱导血管内皮细胞凋亡的研究[J]. 中国药物与临床, 2016, 16(8): 1127-1129. doi: 10.11655/zgywylc2016.08.011.
[15] Zhang X, Li ZZ, Liu DF, et al. Effects of probucol on hepatic tumor necrosis factor-α, interleukin-6 and adiponectin receptor-2 expression in diabetic rats[J]. Journal of Gastroenterology and Hepatology, 2009, 24(6): 1058-1063. doi: 10.1111/j.1440-1746.2008.05719.x.
[16] 胡建廷, 王汝霞, 高聆. 西洛他唑对糖尿病大鼠视网膜ICAM-1和VCAM-1的影响及其机制[J]. 中国新药杂志, 2011, 20(1): 60-63. HU Jianting, WANG Ruxia, GAO Ling. Cilostazol decreases the expression of ICAM-1 and VCAM-1 in the retina via up-regulating PPAR-γ in diabetic rats[J]. Chinese Journal of New Drugs, 2011, 20(1): 60-63.
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