Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2026, Vol. 40 ›› Issue (3): 80-86.doi: 10.6040/j.issn.1673-3770.0.2025.278

• Original Article • Previous Articles     Next Articles

The effect of local micro-pressure oxygen on the repair and oxidative stress of rabbit corneal epithelial cells after injury

WANG Zhiyuan1,2, HAO Zhongkai2, ZHANG Chenming1,2   

  1. 1. Clinical Medical College, Shandong Second Medical University, Weifang 261053, Shandong, China2. Department of OphthalmoLogy, Jinan Second People's Hospital, Jinan 250021, Shandong, China
  • Published:2026-05-22

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Abstract: Objective To investigate the impact of topical microbaric oxygen therapy on corneal epithelial wound healing and ultrastructural alterations in rabbits, and to elucidate its modulatory effects on corneal oxidative stress levels. Methods A total of 75 healthy adult New Zealand White rabbits(150 eyes)were enrolled to establish a corneal epithelial defect model. Animals were randomly assigned to three groups(n=25 rabbits/50 eyes each). Group A received topical microbaric oxygen therapy for 2h daily; Group B received normobaric oxygen at identical flow and concentration for the same duration; Group C was left to heal spontaneously. Corneal epithelial ultrastructure was evaluated by transmission electron microscopy. Residual epithelial defects were planimetrically quantified with Image-J software. Corneal oxidative stress was assessed by measuring malondialdehyde concentration via enzyme-linked immunosorbent assay. Results At 12, 24, and 36 h post-injury, a significant interaction between group and time was detected for the residual corneal epithelial defect area(Ftime=839,828.012, P<0.001; Fgroup=38,866.823, P<0.001; Ftime*group=7,338.970, P<0.001); the defect consistently ranked AFgroup=0.172, P=0.843; Ftime*group=1.572, P=0.226). Conclusion Topical microbaric oxygen therapy significantly accelerates corneal epithelial wound closure without inducing a measurable increase in corneal oxidative stress.

Key words: Topical microbaric oxygen therapy, Corneal epithelial cells, repair, Oxidative stress, Transmission electron microscope

CLC Number: 

  • R772.2
[1] Sridhar MS. Anatomy of cornea and ocular surface[J]. Indian J OphthalmoL, 2018, 66(2): 190-194. doi: 10.4103/ijo.IJO_646_17
[2] van der Putten C, van den Broek D, Kurniawan NA. Myofibroblast transdifferentiation of keratocytes results in slower migration and lower sensitivity to mesoscale curvatures[J]. Front Cell Dev Biol, 2022, 10: 930373. doi: 10.3389/fcell.2022.930373
[3] An X, Wang G, Jin M, et al. Novel cell culture paradigm prolongs mouse corneal epithelial cell proliferative activity in vitro and in vivo[J]. Front Cell Dev Biol, 2021, 9: 675998. doi: 10.3389/fcell.2021.675998
[4] Menzel-Severing J, Seiler TG, Streit T, et al. Hyperbaric oxygenation maintains elevated stromal oxygen availability during corneal collagen crosslinking with and without epithelial removal[J]. Curr Eye Res, 2024, 49(11): 1131-1137. doi: 10.1080/02713683.2024.2372787
[5] Hsu HT, Yang YL, Chang WH, et al. Hyperbaric oxygen therapy improves Parkinson's disease by promoting mitochondrial biogenesis via the SIRT-1/PGC-1α pathway[J]. BiomoLecules, 2022, 12(5): 661. doi: 10.3390/biom12050661
[6] 周春阳,袁正,李依静,等. 美洲大蠊提取液对兔角膜上皮损伤修复影响的实验研究[J]. 中医眼耳鼻喉杂志, 2023, 13(4): 181-184. doi:10.3969/j.issn.1674-9006.2023.04.001
[7] Loo S, Kam A, Tam JP. Hyperstable EGF-like bleogen derived from cactus accelerates corneal healing in rats[J]. Front Pharmacol, 2022, 13: 942168. doi: 10.3389/fphar.2022.942168
[8] Luo Y, Liu L, Liao Y, et al. Multifunctional baicalin-modified contact lens for preventing infection, regulating the ocular surface microenvironment and promoting corneal repair[J]. Front Bioeng Biotechnol, 2022, 10: 855022. doi: 10.3389/fbioe.2022.855022
[9] Wang Y, Kang H, Jin M, et al. Phenotypic and transcriptomics analyses reveal underlying mechanisms in a mouse model of corneal bee sting[J]. Toxins(Basel), 2022, 14(7): 468. doi: 10.3390/toxins14070468
[10] Li L, Li L, Chen Q, et al. Toxicity evaluation of long-term topical application of recombinant human keratinocyte growth factor-2 eye drops on macaca fascicularis[J]. Front Pharmacol, 2021, 12: 740726. doi: 10.3389/fphar.2021.740726
[11] 王文莹, 冯珺, 邓世靖, 等. 自体血清点眼对神经营养性角膜病变持续角膜上皮缺损的疗效评估[J]. 中华实验眼科杂志, 2024, 42(2): 143-151. doi: 10.3760/cma.j.cn115989-20221213-00584
[12] Wu MF, Peng X, Zhao JL, et al. Mitophagy and mitochondrion-related expression profiles in response to physiological and pathological hypoxia in the corneal epithelium[J]. Genomics, 2023, 115(6): 110739. doi: 10.1016/j.ygeno.2023.110739
[13] Li S, Ding G, Sun Y, et al. Impact of different oxygen supply methods on the healing of corneal epithelial wound and the level of acetylcholine[J]. J OphthalmoL, 2021:4737479. doi: 10.1155/2021/4737479
[14] Haubner F, Muschter D, Pohl F, et al. A co-culture model of fibroblasts and adipose tissue-derived stem cells reveals new insights into impaired wound healing after radiotherapy[J]. International Journal of moLecular Sciences, 2015, 16(11): 25947-25958. doi: 10.3390/ijms161125935
[15] Mcmonnies CW. Hyperbaric oxygen therapy and the possibility of ocular complications or contraindications[J]. Clin Exp Optom, 2015, 98(2): 122-125. doi: 10.1111/cxo.12203
[16] 王磊, 王学艺, 田艳娟, et al. 高压氧治疗COVID-19后视神经炎1例及文献分析[J]. 山东大学耳鼻喉眼学报, 2024, 38(5): 100-104. doi:10.6040/j.issn.1673-3770.0.2023.277 WANG Lei, WANG Xueyi, TIAN Yanjuan, et al. Hyperbaric oxygen therapy for optic neuritis after COVID-19: a case report and literature analysis[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(5): 100-104. doi:10.6040/j.issn.1673-3770.0.2023.277
[17] Troitskaya O, Novak D, Nushtaeva A, et al. EGFR transgene stimulates spontaneous formation of MCF7 breast cancer cells spheroids with partly loss of HER3 receptor[J]. Int J moL Sci, 2021, 22(23): 12937. doi: 10.3390/ijms222312937
[18] Loma P, Guzman-Aranguez A, Pérez de Lara MJ, et al. Diadenosine tetraphosphate induces tight junction disassembly thus increasing corneal epithelial permeability[J]. Br J Pharmacol, 2015, 172(4): 1045-1058. doi: 10.1111/bph.12972
[19] 倪梦圆,廖荣丰. 糖尿病大鼠病程不同阶段角膜上皮紧密连接蛋白claudin-1表达的变化[J]. 安徽医科大学学报, 2016, 51(7): 961-964
[20] 苏海莹. MUC1调控细胞间紧密连接对糖尿病角膜上皮损伤后修复的影响[D]. 广东:南方医科大学, 2024
[21] Li S, Tian Q, Ding G, et al. The impact of different oxygen delivery methods on corneal epithelial repair after injury[J]. J OphthalmoL, 2022: 3260087. doi: 10.1155/2022/3260087
[22] Smith HL, Bourne JN, Cao G, et al. Mitochondrial support of persistent presynaptic vesicle mobilization with age-dependent synaptic growth after LTP[J]. Elife, 2016, 5: e15275. doi: 10.7554/eLife.15275
[23] Schwarz DS, Blower MD. The endoplasmic reticulum: structure, function and response to cellular signaling[J]. Cell moL Life Sci, 2016, 73(1): 79-94. doi: 10.1007/s00018-015-2052-6
[24] Shyu KG, Wang BW, Fang WJ, et al. Hyperbaric oxygen-induced long non-coding RNA MALAT1 exosomes suppress MicroRNA-92a expression in a rat model of acute myocardial infarction[J]. J Cell moL Med, 2020, 24(22): 12945-12954. doi: 10.1111/jcmm.15889
[25] 周燕, 雷佳玉, 谢艳. 高压氧对输尿管梗阻大鼠的肾病理组织及氧化应激损伤的作用机制[J]. 国际泌尿系统杂志, 2025, 45(1): 107-112. doi:10.3760/cma.j.cn431460-20230625-00026
[26] Bonilha VL, Bell BA, Rayborn ME, et al. Absence of DJ-1 causes age-related retinal abnormalities in association with increased oxidative stress[J]. Free Radic Biol Med, 2017, 104: 226-237. doi: 10.1016/j.freeradbiomed.2017.01.018
[27] Lee MJ, Seo HJ, Hwang GS, et al. moLecular mechanism of cinnamomum cassia against gastric damage and identification of active compounds[J]. Biomo Lecules, 2022, 12(4):525. doi: 10.3390/biom12040525
[28] 刘萱, 张婕, 刘小毛, 等. PM2.5对人角膜上皮细胞屏障功能、氧化应激反应以及自噬的影响[J]. 现代生物医学进展, 2024, 24(22): 4207-4213. doi:10.13241/j.cnki.pmb.2024.22.002
[29] Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde(MDA)and relatives in biological samples: Analytical and biological challenges[J]. Anal Biochem, 2017, 524: 13-30. doi: 10.1016/j.ab.2016.10.021
[30] Islam MT. Oxidative stress and mitochondrial dysfunction-linked neurodegenerative disorders[J]. Neurol Res, 39(1): 73-82. doi: 10.1080/01616412.2016.1251711
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