JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY)

• Articles • Previous Articles     Next Articles

Expression of the heat shock protein 27 in rat cochlea induced by sodium salicylate injection by the FQ-PCR technique

FAN Qi-jun1, HUANG Zhi-wu2, MEI Ling2, XIAO Bo-kui2   

  1. 1. Department of Otolaryngology, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China; 2. Department of Otolaryngology & Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China
  • Received:2008-02-19 Revised:2008-04-23 Online:2008-06-16 Published:2008-06-16
  • Contact: HUANG Zhi-wu

PDF (PC)

1274

Abstract: To investigate the gene expression of HSP27 in the cochlea of rats induced by sodium salicylate injection and to explore its role in sodium salicylate ototoxity. MethodsThe HSP27 gene expression in specimens was determined by using real time RT-PCR with SYBR GREEN I, and β-actin served as the internalcontrol. ResultsThe gene expression of HSP27 in the cochlea of rats induced by sodium salicylate injection was significantly increased compared with normal rats(P＜0.01). ConclusionQuantitative PCR with SYBR GREEN I fluorescence is a reliable method to determine the gene expression of rarely available tissues. Sodium salicylate has an effect on inducing the gene expression of HSP27 in the cochlea of rats.

Key words: Cochlea, Gene, Heat shock protein 27, Sodium salicylate, Rat

CLC Number: 

  • R764
[1] WANG Min, LIU Hao, JIANG Luhan, TAN Liyue, LI Wen, FU Xiaolong. Packaging of AAV-ie-Tnfaip8l2 and its effect on improving auditory function in Tnfaip8l2-/- mice [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(3): 7-15.
[2] LI Shiyun, XIE Yan, PAN Jiayu, CHEN Meixin, LONG Dan, ZHANG Chunlin. Meta analysis of clinical efficacy of cochlear implant in elderly patients with postlingual deafness [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(3): 20-30.
[3] WU Fulian, JI Xuying. Layer-specific response distribution characteristics of binaural integration neurons in the auditory cortex and synaptic input regulation mechanism [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(2): 7-17.
[4] WANG Kaijian, WANG Wei, CHEN Xuesheng, FAN Chunsun, HE Yingying, HUANG Dongling, QIAN Cunhui, DING Shujun. Correlation between neutrophil-to-lymphocyte ratio with hearing loss and audiometric thresholds in adults: a cross-sectional study based on NHANES [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(2): 18-28.
[5] WANG Nuan, WANG Xinyang, JI Changhao, LIU Min, GAO Jiangang, SUN Jin. In vivo proximity labeling of cell-surface proteins in mouse cochlear hair cells [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 6-12.
[6] ZHANG Nana, HUANG Guanjiang, LU Biaoqing. Analysis of influencing factors for laryngeal carcinogenesis and construction of a predictive model [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 29-37.
[7] PAN Ruolin, LIAO Xuan, LAN Changjun, TAN Qingqing, XIE Lixuan, HUANG Huan, QIN Suyun, WANG Yan. Experimental study on the influence of decentration and tilt on the optical properties of monofocal intraocular lens with different diopter [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 38-46.
[8] LIU Yijie, LU Xiuzhen, WU Qiuxin. Research rogress of exosomes in the pathogenesis, diagnosis and treatment in ophthalmic diseases [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 135-141.
[9] GAO Juanjuan, XU Jia, CHEN Wenjing, LIANG Sichao, GUO Zhenping, CHEN Junyan, GUO Xiang, YI haijin. Subtotal resection of facial nerve schwannoma with preservation of facial nerve function [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 8-16.
[10] LI Kang, LI Xuanyi, LIU Wen. Diagnosis and treatment of chronic suppurative otitis media combined with fungal infection of the external auditory canal [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 23-30.
[11] CHEN Tingyi, GONG Hui, CHEN Liang, LIANG Ping, WANG Xian, LIU Yaling, HU Yarou,ZHANG Guoming. Analysis of factors affecting detection in quantitative visual assessment of infants [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 118-125.
[12] CHEN Tao, JIANG Yang, LI Ying. SMILE correction of refractive regression after PRK surgery: a case report and literature review [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 148-153.
[13] LU Jiantao, ZHONG Jie, LIU Shaofeng. Electrophysiological characteristics of deep projections from bilateral auditory cortex [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 1-7.
[14] LIU Xin, DU Anshi, WANG Yan, TAN Qingqing, LI Junping, GUO Yunan, LAN Changjun. Investigation of the correlation between levator palpebrae superioris muscle strength and postoperative eyelid closure in patients with blepharoptosis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 70-75.
[15] SHEN Wenhao, CHEN Xi. Application of dual-energy CT in the laryngeal squamous cell carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 156-160.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd