JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY)

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Differentially expressed genes in laryngeal squamous cell carcinoma and normal tissue by cDNA microarray

LIU Rui1, SHI Wen-jie2, LIU Ji-xiang2   

  1. 1. Department of Biology Science, Shandong University, Jinan 250100, Shandong, China;2. Department of Otolaryngology, Tianjin First Central Hospital, Tianjin 300192
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24
  • Contact: LIU Rui

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Abstract: Objective: To screen for the differentially expressed genes in laryngeal squamous carcinoma cells and normal laryngeal tissues using cDNA microarray. Methods: The samples from two laryngeal squamous carcinoma tissues were tested using DNA microarrays. The DNAs were fixed on a glass plate by a series of treatments. The mixed probes were then hybridized to DNA microarrays and scanned for fluorescent signals. Differences between the two tissues were produced. Results: Ran belonging to Ras gene family increased 12.207 times, RAB32 belonging to Ras gene family increased 4.904 times and BIRC5 (Survivin) increased 15.495 times. They were significantly highly expressed in laryngeal cancer. The GFI1B gene decreased 0.0265 times. The GATA1 gene was significantly lowly expressed in one laryngeal cancer and was not expressed in carcinoma cells of another patient. Conclusion: cDNA microarray made in our Lab can recognize more than 7000 gene differences between cancer tissues and normal tissues at the same time and find gene variation. These results accord with other studys.

Key words: Squamous cell carcinoma, Laryngeal neoplasms, Gene expression, DNA microarray

CLC Number: 

  • R739.65
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