山东大学耳鼻喉眼学报

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鼻腔鼻窦恶性肿瘤细胞周期的DNA分析

公 蕾1,孙 洁1,薛子超2,李敬华3,薛卫国1   

  1. 1. 青岛大学附属青岛市市立医院耳鼻咽喉-头颈外科, 山东 青岛 266011;2. 中南大学湘雅医学院2005级临床医学, 长沙 410008; 3. 青岛大学附属青岛市市立医院病理科, 山东 青岛 266011
  • 收稿日期:2008-04-02 修回日期:2008-06-05 出版日期:2008-06-16 发布日期:2008-06-16
  • 通讯作者: 孙洁

DNA analysis of the cell cycle in sino-nasal neoplasm

GONG Lei1, SUN Jie1, XUE Zi-chao2, LI Jing-hua3, XUE Wei-guo1   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao 266011, Shandong, China; 2. Department of Clinical Medicine, Grade 2005, Xiangya School of Medicine, Central South University, Changsha 410008, China; 3. Department of Pathology, Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao 266011, Shandong, China
  • Received:2008-04-02 Revised:2008-06-05 Online:2008-06-16 Published:2008-06-16
  • Contact: SUN Jie

摘要: 目的 探讨流式细胞术检测细胞周期在鼻腔鼻窦恶性肿瘤组织中的意义。方法 取石蜡包埋的30例鼻腔鼻窦恶性肿瘤作实验组,另取30例鼻中隔偏曲患者的正常黏膜组织为对照组,用流式细胞术检测各组的DNA指数(DNA index, DI)及S期细胞百分数(S-phase fraction,SPF),研究各指数与组织良恶性之间的相关性。结果 DI与SPF在恶性组织与正常对照组之间差异均有统计学意义。结论流式细胞术检测DI、SPF在鼻腔鼻窦恶性肿瘤的诊断中有重要意义。

关键词: 流式细胞术, DNA指数, S期细胞百分数, 鼻窦肿瘤

Abstract: To explore the significance of the cell cycle in sino-nasal neoplasm by flow cytometry (FCM). Methods30 cases of paraffin-embedded malignant tumors in nasal cavities or sinuses served as the experimental group and 30 cases of normal mucosa of deflection of the nasal septum as the control group. The DNA index(DI) value and the S-phase fraction(SPF) values were determined by FCM and their correlation was analyzed. ResultsThe DI and SPF values of the experimental group were significantly higher than those of the control group. ConclusionFlow cytometric analysis of DI and SPF plays an important role in diagnosis of sino-nasal neoplasm.

Key words: DNA index, S-phase fraction tumor, Flow cytometry, Sinus neoplasms

中图分类号: 

  • R730.21
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