快速老化小鼠的听功能和耳蜗螺旋神经元的增龄性变化

山东大学耳鼻喉眼学报

快速老化小鼠的听功能和耳蜗螺旋神经元的增龄性变化

刘强和^1, 罗香林¹，耿宛平¹，陈晨²，雷迅¹，刘芳贤¹，邓明¹

1. 桂林医学院附属医院耳鼻咽喉-头颈外科，广西桂林 541001；2. 澳大利亚亨利王子医学研究所、Monash大学医学院生理系，澳大利亚维多利亚 3168

收稿日期:2008-03-07 修回日期:2008-04-18 出版日期:2008-06-16 发布日期:2008-06-16
通讯作者: 刘强和

Age-related spiral ganglion neuron damages and hearing loss in senescence accelerated mice

LIU Qiang-he¹, LUO Xiang-lin¹, GENG Wan-ping¹, CHEN Chen², LEI Xun¹, LIU Fang-xian¹, DENG Ming¹

1. Department of Otorhinolaryngology & Head and Neck Surgery, Affiliated Hospital of Guilin Medical College, Guilin 541001, China; 2. Prince Henry′s Institute of Medical Research, Department of
Physiology of Monash University, Victoria 3168, Australia

Received:2008-03-07 Revised:2008-04-18 Online:2008-06-16 Published:2008-06-16
Contact: LIU Qiang-he

摘要/Abstract

摘要： 目的探讨快速老化小鼠听觉功能和耳蜗螺旋神经元的增龄性变化。方法选取1、3、5、7、9月龄的快速老化小鼠亚系1( Senescence accelerated mouse/prone 1, SAMP 1)作为实验组，而同龄抗快速老化小鼠亚系1(Senescence accelerated mouse/resistance 1, SAMR 1) 作为SAMP 1的正常对照组。分别观察其8kHz短纯音听觉脑干反应阈值、耳蜗螺旋神经节细胞透射电镜形态学、末端脱氧核苷酸转移酶介导的dUTP原位切口末端标记［terminal deoxynucleotidyl transferase(TdT) dUTP nick end labeling］ TUNEL免疫组化染色等方面的增龄性变化。结果① 听功能检测。第7、9个月龄SAMP 1小鼠跟同龄SAMR 1小鼠比较听觉脑干反应阈值差异有统计学意义；② 耳蜗螺旋神经节细胞形态学检测。第7、9个月龄SAMP 1小鼠可见螺旋神经元凋亡，而同龄SAMR 1小鼠罕见螺旋神经元凋亡；③ 耳蜗中轴位切片TUNEL染色。第7个月龄SAMR 1小鼠SGN细胞核基本上不着色［TUNEL染色阳性率为（2.27±2.43）%］，第7个月龄SAMP 1小鼠部分SGN胞核着色［染色阳性率为（11.36±4.96）%］，两者的染色阳性率差异有统计学意义。结论SAMP 1小鼠随月龄增长耳蜗螺旋神经元凋亡、听功能减退，7月龄SAMP 1小鼠即出现明显的听功能老化特征，可作为老年聋动物模型用于耳聋的相关研究。

关键词: 快速老化小鼠, 听觉脑干反应阈值, 螺旋神经元, 凋亡

Abstract: To determine age-related spiral ganglion neuron damages and hearing loss in the senescence accelerated mice. MethodsThe auditory thresholds and the morphological changes of the spiral ganglion neurons were studied in senescence accelerated mouse/prone 1 (SAMP 1) mice of 1, 3, 5, 7 and 9 months. Normal aging senescence accelerated mouse/resistance 1 (SAMR 1) mice served as the control group. 8?kHz threshold of auditory brain-stem response was determined, transmission electron microscopy and terminal deoxynucleotidyl transferase(TdT) dUTP nick end labeling(TUNEL) were used to observe the auditory function changes, the morphological changes and the immunohistochemical changes. ResultsAuditory function: Compared with the SAMR 1 mice, SAMP 1 mice of 7 or 9 months developed a progressive hearing loss at 8kHz，which showed an age-related significant increase(P＜0.05). Morphological observation: The SAMR 1 mice had no apoptosis in the spiral ganglion neurons (SGNs), while the SAMP 1 mice of 7 or 9 months developed a significant apoptosis in the SGNs. TUNEL observation: Negative immunohistochemical reaction for TUNEL staining was found in the SGNs in the SAMR 1 mice of 7 months, and a positive reaction was found in the nucleolus of the part of the SGNs of the SAMP 1 mice of 7 months. The image analysis showed that the percentage of positive SGNs of the SAMP 1 mice of 7 months was significantly higher than that of the SAMR 1 mice of 7 months. ConclusionsSAMP 1 mice can be used for investigating mechanism of auditory-function-aging and as a pertinent animal model of presbyacousis.

Key words: Threshold of the auditory brain stem response, Spiral ganglion neuron, Senescence accelerated mouse, Apoptosis

中图分类号:

R764.35

刘强和,罗香林,耿宛平,陈晨,雷迅,刘芳贤,邓明 . 快速老化小鼠的听功能和耳蜗螺旋神经元的增龄性变化[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 215-217 .

LIU Qiang-he,LUO Xiang-lin,GENG Wan-ping,CHEN Chen,LEI Xun,LIU Fang-xian,DENG Ming . Age-related spiral ganglion neuron damages and hearing loss in senescence accelerated mice[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 215-217 .

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