山东大学耳鼻喉眼学报

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大鼠阻塞性睡眠呼吸暂停综合征动物模型的建立

柳忠禄,王岩,李延忠
  

  1. (山东大学齐鲁医院耳鼻咽喉头颈外科、卫生部耳鼻喉科学重点实验室, 济南 250012)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-04-16 发布日期:2009-04-16
  • 通讯作者: 李延忠

Animal model of obstructive sleep apnea syndrome in rats

LIU Zhonglu, WANG Yan, LI Yanzhong
  

  1. (Department of Otorhinolaryngology & Head and Neck Surgery, Qilu Hospital of Shandong University,
    Key Laboratory of Otorhinolaryngology of Health Ministry, Jinan 250012, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-04-16 Published:2009-04-16
  • Contact: LI Yanzhong

摘要: 目的建立一个稳定、可靠的阻塞性睡眠呼吸暂停综合征(OSAS)动物模型。方法将8只Wistar大鼠的双侧舌腭弓、咽腭弓及舌根处注射透明质酸钠,分别于注射前及注射后4周,以浅状态模拟睡眠状态,用多导睡眠仪监测动物的脑电、肌电及口鼻气流。结果① 动物处于浅麻醉状态时,脑电和肌电活动与睡眠时相似;② 注射前和注射后4周,动物在监测过程中均出现伴有胸腹反常运动的呼吸暂停,但是注射前后呼吸暂停指数(AI)及最长呼吸暂停时间之间差异有统计学意义(P<0.05)。结论咽部注射透明质酸钠法所建立的大鼠阻塞性睡眠呼吸暂停综合征模型稳定、可靠,与人类OSAS相似,可应用于OSAS的发病机制、病理生理改变和并发症等方面的研究。

关键词: 睡眠呼吸暂停, 阻塞性, 大鼠, Wistar, 动物模型

Abstract: To build up a steady and credible animal model of obstructive sleep apnea syndrome(OSAS). MethodsSodium Hyaluronate was injected into the palatoglossal arch, palatopharyngeal arch and root of the tongue of eight Wistar rats. An electroencephalogram, electromyogram and oronasal airflow were recorded by PSG before and 4 weeks after injection when rats were under light anesthesia, which was expected to be similar to the sleep state. ResultsWhen the animals were under light anesthesia, the electroencephalogram and electromyogram were similar to the sleep state. All animals showed apnea with unusual movements of the thorax and the abdomen before and 4 weeks after injections. Differences between apnea index(AI) and the longest apnea before and after injection of Sodium Hyaluronate was obvious(P<0.05). ConclusionAn animal model of OSAS in rats established by injection of Sodium Hyaluronate was steady, credible and similar to OSAS of human beings. It can be used to study the pathogenesis, pathological and physiological changes, and complication of OSAS.

中图分类号: 

  • R766.9
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