山东大学耳鼻喉眼学报 ›› 2019, Vol. 33 ›› Issue (5): 69-72.doi: 10.6040/j.issn.1673-3770.0.2018.425

• 论著 • 上一篇    下一篇

长链非编码RNA在鼻咽癌组织中表达及其生物学功能研究

李林1,张衡2,冼宇飞3,盛晓丽3()   

  1. 1. 广东省人民医院珠海医院 珠海市金湾中心医院耳鼻喉头颈外科, 广东 珠海 519040
    2. 广州医科大学附属第一医院病理科，广东 广州 510080
    3. 广东省医学科学院 广东省人民医院耳鼻咽喉头颈外科，广东 广州 510080
  • 收稿日期:2018-09-26 修回日期:2018-11-08 出版日期:2019-09-20 发布日期:2019-10-15
  • 通讯作者: 盛晓丽 E-mail:shengzhang2016@126.com

Expression and biological function of long chain non-coding cytoskeleton regulator RNA in nasopharyngeal carcinoma

Lin LI1,Heng ZHANG2,Yufei XIAN3,Xiaoli SHENG3()   

  1. 1. Department of Otorhinolaryngology Head and Neck Surgery, Zhuhai Hospital of Guangdong Provincial People's Hospital，Jinwan Central Hospital, Zhuhai 519040, Guangdong, China
    2. Department of Pathology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510080, Guangdong, China
    3. Department of Otorhinolaryngology Head and Neck Surgery, Guangdong Academy of Medical Sciences, Guangdong Province People's Hospital, Guangzhou 510080, Guangdong, China
  • Received:2018-09-26 Revised:2018-11-08 Online:2019-09-20 Published:2019-10-15
  • Contact: Xiaoli SHENG E-mail:shengzhang2016@126.com

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摘要： 目的

探讨长链非编码RNA (cytoskeleton regulator RNA, CYTOR)在鼻咽癌组织中表达及其生物学功能。

方法

实时荧光定量PCR检测32例鼻咽癌组织及28例鼻咽部良性病变患者黏膜组织中CYTOR的表达情况；Real-time PCR检测人鼻咽上皮细胞（NP69）和鼻咽癌细胞(CNE1、HONE1)中CYTOR的表达情况；应用小干扰RNA抑制CNE1、 HONE1细胞中CYTOR中的表达，并采用MTT、 Transwell 和划痕实验分别检测下调CYTOR表达后鼻咽癌细胞(CNE1， HONE1)的增殖能力、迁移能力变化情况。

 结果

CYTOR在鼻咽癌组织及鼻咽癌细胞系（CNE1、HONE1）中表达水平高于鼻咽部良性病变患者黏膜组织和人鼻咽上皮细胞（NP69），而瞬时抑制CYTOR表达，鼻咽癌细胞系（CNE1、HONE1）的增殖与迁移能力降低，差异均有统计学意义(P <0.05)。

 结论

CYTOR在鼻咽癌组织及鼻咽癌细胞系中高表达，并与鼻咽癌的增殖和迁移能力有关。

关键词: 细胞骨架调节RNA, 鼻咽癌, 侵袭, 转移

Abstract: Objective

To investigate the expression and biological function of long non-coding cytoskeleton regulator RNA (CYTOR) in nasopharyngeal carcinoma.

Methods

Real-time PCR was used to examine CYTOR expression in nasopharyngeal carcinoma tissues (n = 32), nasopharyngeal carcinoma cell lines (CNE1 and HONE1), 28 patients with benign nasopharyngeal lesions, and human nasopharyngeal epithelial cells (NP69). Moreover, CYTOR expression in the CNE1 and HONE1 cell lines was knocked down using RNAi assay. MTT, Transwell, and wound healing assays were also used to detect the proliferation and migration of nasopharyngeal carcinoma cells from CNE1 and HONE1 after downregulating CYTOR expression.

 Results

CYTOR expression in the nasopharyngeal carcinoma tissues and nasopharyngeal carcinoma cell lines was observed to be significantly higher than that in the nasopharyngeal benign lesion mucosal tissues and NP69. After transient inhibition of CYTOR expression, the migration and proliferation abilities of the nasopharyngeal carcinoma cell lines were also observed to decrease significantly (P < 0.05).

 Conclusion

CYTOR expression is elevated in pharyngeal cancer tissues and nasopharyngeal carcinoma cell lines and is positively correlated with the migration ability of nasopharyngeal carcinoma.

Key words: cytoskeleton regulator RNA, Nasopharyngeal Carcinoma, Invasion, Metastasis

中图分类号: 

  • R739.6

表1

下调CYTOR表达抑制鼻咽癌细胞的迁移能力"

时间 组别 宽度 F P
0 h 0.22 0.81
CNE1 406.32±22.11
CNE1_SiNC 411.36±19.21
CNE1_SiCYTOR 414.21±26.06
24 h 46.69 <0.001
CNE1 102.11±26.21
CNE1_SiNC 116.65±30.14
CNE1_SiCYTOR 336.45±45.69

图1

抑制CYTOR表达对鼻咽癌细胞侵袭能力的影响"

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