山东大学耳鼻喉眼学报 ›› 2019, Vol. 33 ›› Issue (5): 69-72.doi: 10.6040/j.issn.1673-3770.0.2018.425
Lin LI1,Heng ZHANG2,Yufei XIAN3,Xiaoli SHENG3()
摘要: 探讨长链非编码RNA (cytoskeleton regulator RNA, CYTOR)在鼻咽癌组织中表达及其生物学功能。 实时荧光定量PCR检测32例鼻咽癌组织及28例鼻咽部良性病变患者黏膜组织中CYTOR的表达情况;Real-time PCR检测人鼻咽上皮细胞(NP69)和鼻咽癌细胞(CNE1、HONE1)中CYTOR的表达情况;应用小干扰RNA抑制CNE1、 HONE1细胞中CYTOR中的表达,并采用MTT、 Transwell 和划痕实验分别检测下调CYTOR表达后鼻咽癌细胞(CNE1, HONE1)的增殖能力、迁移能力变化情况。 CYTOR在鼻咽癌组织及鼻咽癌细胞系(CNE1、HONE1)中表达水平高于鼻咽部良性病变患者黏膜组织和人鼻咽上皮细胞(NP69),而瞬时抑制CYTOR表达,鼻咽癌细胞系(CNE1、HONE1)的增殖与迁移能力降低,差异均有统计学意义(P <0.05)。 CYTOR在鼻咽癌组织及鼻咽癌细胞系中高表达,并与鼻咽癌的增殖和迁移能力有关。
中图分类号:
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