山东大学耳鼻喉眼学报 ›› 2024, Vol. 38 ›› Issue (3): 18-25.doi: 10.6040/j.issn.1673-3770.0.2022.219
• 论著 • 上一篇
杨开炎1, 唐凤珠2, 覃启才1, 李旭祥3, 冯大益3, 农丰靖1, 杨秋云1
YANG Kaiyan1, TANG Fengzhu2, QIN Qicai1, LI Xuxiang3, FENG Dayi3, NONG Fengjing1, YANG Qiuyun1
摘要: 目的 通过免疫组化染色的方法研究异常纺锤体样小头畸形相关蛋白(abnormal spindle-like microcephaly associated protein, ASPM)在鼻咽癌组织样本中的表达情况,进一步分析ASPM蛋白表达与鼻咽癌患者临床病理参数和预后的关系,探讨ASPM mRNA在鼻咽癌中的表达情况。 方法 从GEO数据库下载鼻咽癌的芯片数据集,使用R语言对鼻咽癌芯片数据集进行差异表达分析;使用免疫组化染色方法分析ASPM蛋白在鼻咽癌中的差异表达;使用卡方检验的方法分析ASPM表达与鼻咽癌患者临床病理参数的相关性;采用生存曲线分析ASPM表达与鼻咽癌患者预后的关系;采用Cox回归模型分析影响鼻咽癌患者预后的危险因素;使用受试者工作特征(receiver operator characteristic, ROC)曲线评估各种预后参数预测鼻咽癌患者预后的效能。 结果 通过鼻咽癌芯片数据分析发现ASPM mRNA在鼻咽癌芯片数据集中高表达(P<0.001);免疫组化染色结果显示鼻咽癌组织中ASPM蛋白表达明显高于鼻咽炎组织(P<0.001),ASPM表达与T分期(P=0.048)、N分期(P=0.050)及总临床分期(P=0.029)相关,高表达ASPM蛋白组患者总生存期短于低表达ASPM蛋白组患者(P<0.001);通过多因素Cox回归模型分析,显示ASPM表达水平可能为影响鼻咽癌患者预后的独立危险因素(P=0.015);通过ROC曲线分析,显示联合总临床分期及ASPM表达用于预测鼻咽癌患者预后具有更高的准确性(P<0.001)。 结论 ASPM在鼻咽癌组织样本中高表达,其高表达与鼻咽癌的恶性程度、疾病进展及较差的预后相关,可能成为鼻咽癌中有价值的预测预后的生物标志物。联合总临床分期及ASPM表达用于预测鼻咽癌患者预后可能是一种有前景的方法。
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