山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (1): 64-68.doi: 10.6040/j.issn.1673-3770.0.2013.268

• 论著 • 上一篇    下一篇

络治法对STZ诱导的糖尿病大鼠视网膜血管消化铺片中VCAM-1表达的影响

马栋, 郭承伟   

  1. 山东中医药大学附属医院眼科, 山东 济南 250011
  • 收稿日期:2013-09-22 出版日期:2015-02-16 发布日期:2015-02-16
  • 通讯作者: 郭承伟。E-mail:guocw12009@163.com E-mail:guocw12009@163.com
  • 作者简介:马栋。E-mail:adong79@163.com
  • 基金资助:
    山东省自然科学基金(ZR2012HM028)

Collaterals therapy influences the expression of VCAM-1 in the retinal capillary wall of STZ-induced diabetic rats

MA Dong, GUO Cheng-wei   

  1. Department of Ophthalmology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, Shandong, China
  • Received:2013-09-22 Online:2015-02-16 Published:2015-02-16

PDF (PC)

480

摘要： 目的 观察络治法对链佐脲菌素(STZ)诱导的糖尿病大鼠视网膜毛细血管消化铺片中血管细胞黏附分子-1(VCAM-1)表达的影响,探讨络治法对糖尿病视网膜病变微血管损伤的保护作用及机制。方法 将60只雌性Wistar大鼠随机分成空白组、模型组、对照组及络治组。除空白组不做处理外,其余各组均制作糖尿病大鼠模型并按方案给药。给药24周后每组大鼠取双侧眼球做视网膜血管消化铺片,右眼PAS染色做形态学观察,左眼免疫荧光染色法测定VCAM-1在血管壁中的表达。结果 PAS染色结果显示,络治组糖尿病大鼠视网膜毛细血管在管径粗细、局部扩张程度、扭曲聚集程度等方面较对照组及模型组有明显改善,且络治组大鼠毛细血管管壁周细胞的丢失明显减少,组间比较有统计学差异(P<0.05)。免疫荧光染色显示,络治组视网膜血管壁中VCAM-1呈较少的表达,组间比较有统计学差异(P<0.05)。结论 络治法能够抑制VCAM-1在糖尿病视网膜毛细血管壁中的表达,有效减轻高血糖引起的视网膜微血管损伤,改善糖尿病视网膜微血管的结构与机能。

关键词: 大黄虫丸, 糖尿病视网膜病变, 络治法, 大鼠, 血管细胞黏附分子-1

Abstract: Objective To observe the expression of vascular cell adhesion molecule -1 (VCAM-1) in the retinal digest preparations of streptozotocin (STZ)-induced diabetic rats treated with collaterals therapy, and to investigate the protective effects and mechanism of collaterals therapy on diabetic retinopathy microvascular injury. Methods Sixty female Wistar rats were randomly divided into 4 groups including blank group, model group, control group and collaterals therapy group. All except the blank group rats were made into models of diabetic rats and treated according to the scheme. After 24 weeks each rat's bilateral eyes were made into retinal vascular digest preparations. The right eye was treated by PAS staining for morphological observation and the left eye by immunofluorescence staining for determining the VCAM-1 expression in the vessel wall. Results PAS staining showed that collaterals therapy group had obvious improvement on morphological changes such as vessel diameter, local expansion, vascular twist and so on. However, retinal pericyte damage of collaterals therapy group was significantly suppressed (P<0.05). Immunofluorescence staining showed that VCAM-1 in collaterals therapy group had less expression than in the other groups (P<0.05). Conclusion Collaterals therapy can inhibit the expression of VCAM-1 in diabetic retinal capillary wall, effectively alleviate retinal microvascular damage caused by hyperglycemia, and improve diabetic retinal microvascular structure and function.

Key words: Rhubarb eupolyphaga pills, Diabetic retinopathy, Vascular cell adhesion molecule-1, Collaterals therapy, Rats

中图分类号: 

  • R574
[1] Chun-Shen Li, Rong Miao, Qing Cai, et al. The role of ICAM-1 and VCAM-1 in diabetic retinopathy[J]. Int Eye Sci, 2012, 12(1):1-3.
[2] Gustavsson C, Agardh C D, Zetterqvist A V, et al. Vascular cellular adhesion molecule-1 (VCAM-1) expression in mice retinal vessels is affected by both hyperglycemia and hyperlipidemia[J]. PLoS One, 2010, 5(9):e12699.
[3] 徐叔云,卞如濂,陈修,等.药理实验方法学[M].3版.北京:人民卫生出版社, 1982
[4] 潘琳,周水平,蔡晓频,等.糖尿病大鼠视网膜血管铺片技术的改进及形态学观察[J].中国组织化学与细胞化学杂志, 2001, 10(4):456-458.
[5] 雷燕,王永炎,黄启福.论瘀毒阻络是络病形成的病理基础[J].北京中医药大学学报,1999,22(2):8-10. LEI Yan, WANG Yongyan, HUANG Qifu. Pathologic basis of collateral disease formation[J]. Journal of Beijing University of TCM, 1999, 22(2):8-10.
[6] 江玉娟,司秋菊,张艳慧,等.大黄蜇虫丸对动脉粥样硬化大鼠核因子-κB蛋白表达的影响[J].中国老年学杂志,2011,31(1):113-115.
[7] 成家茂,潘志恒,陈海燕.大黄虫丸的现代功用及作用机制[J].云南中医中药杂志,2009,30(2):67-68.
[8] 李文琍,吴诗品.大黄虫丸治疗慢性肝炎肝纤维化的临床疗效观察[J].中国药房,2008,19(21):1658-1660. LI Wenli, WU Shipin. Clinical efficacy of Dahuang Zhechong Pill on chronic hepatitis with hepatic fibrosis[J]. China Pharmacy, 2008, 19(21):1658-1660.
[9] 徐斌.刺络放血疗法的血管生物学基础[J].中国临床康复,2004,8(24):5126-5127.
[10] 张慧敏,费宇彤,时宇静,等.针刺"百会""太阳"改善局灶性脑缺血脑微血管内皮细胞功能的动态观察[J].针刺研究,2006,31(2):67. ZHANG Huimin, FEI Yutong, SHI Yujing, et al. Effects of acupuncture of "Baihui"(GV 20) and"Taiyang"(EX-HN 5) on functions of vascular endothelial cells in cerebral ischemia injury rats[J]. Acupuncture Research, 2006, 31(2):67.
[11] Goldberg R B. Cytokine and cyokine-like inflammation markers,endothelial dysfunction, and imbalanced coagulation in development of diabetes and its complications[J]. Clin Endocrinal Metab, 2009, 94(9):3171-3182.
[12] 李凤鸣.中华眼科学[M].2版.北京:人民卫生出版社, 2004: 2232-2233.
[1] 周学义,李一鸣,王美菊,张苑苑,张历浊. 25+微创玻璃体视网膜手术联合玻璃体腔注射雷珠单抗治疗增生型糖尿病视网膜病变的临床观察[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 87-89.
[2] 周玮琰,王洪亚,杜秀娟,董卫红. 甘糖酯对糖尿病大鼠视网膜病变中血管生成因子VEGF表达的影响[J]. 山东大学耳鼻喉眼学报, 2017, 31(2): 90-95.
[3] 邵娜,张晗. 糖尿病患者行白内障超声乳化术后视力及眼底的变化[J]. 山东大学耳鼻喉眼学报, 2016, 30(1): 83-87.
[4] 李俊英. 瑞舒伐他汀联合非诺贝特对老年糖尿病视网膜病变患者血管内皮功能的影响[J]. 山东大学耳鼻喉眼学报, 2015, 29(5): 72-75.
[5] 刘志高, 颜世龙, 胡尊霞, 孙同鑫, 杨明, 王玉. 济南市农村白内障患者糖尿病及糖尿病视网膜眼底病变患病率调查分析[J]. 山东大学耳鼻喉眼学报, 2015, 29(1): 38-39.
[6] 刘海洋, 李甦雁, 张正培, 范巍. 改良膜分割与双手膜清除技术在23G玻璃体手术治疗增殖性糖尿病视网膜病变中的对比[J]. 山东大学耳鼻喉眼学报, 2015, 29(1): 52-55.
[7] 慕超，王岩,李延忠. 阻塞性睡眠呼吸暂停低通气综合征合并高血压动物模型的建立[J]. 山东大学耳鼻喉眼学报, 2014, 28(2): 1-3.
[8] 刘蓓1,朱忠桥1,杜善双1,王丽丽2,杨新光1. 前期激光及曲安奈德应用对增殖性糖尿病视网膜病变玻璃体切割手术的影响[J]. 山东大学耳鼻喉眼学报, 2013, 27(2): 66-68.
[9] 王淑雅，华宁，李筱荣 . 糖尿病性盲患者玻璃体手术后的脱盲率及未脱盲原因分析           [J]. 山东大学耳鼻喉眼学报, 2011, 25(6): 77-79.
[10] 金慧瑜，黄萍. 可元胶囊治疗单纯型糖尿病性视网膜病变的临床观察[J]. 山东大学耳鼻喉眼学报, 2011, 25(2): 87-.
[11] 方建林，高兴强，赵德安. TLR2、TLR3和TLR4 在变应性鼻炎大鼠鼻黏膜组织中的表达[J]. 山东大学耳鼻喉眼学报, 2010, 24(4): 40-44.
[12] 秦晨，王海波，李建峰，韩月臣，刘闻闻，逯素梅. 大鼠面神经损伤后神经营养因子-4的动态表达[J]. 山东大学耳鼻喉眼学报, 2010, 24(2): 17-.
[13] 张媛媛1，刘得龙2，张庆丰2，陶然1. 生后大鼠下丘GABAA受体β2和γ2亚单位的表达[J]. 山东大学耳鼻喉眼学报, 2010, 24(01): 25-28.
[14] 朱晓凤1，韩月臣1，熊文萍1，逯素梅2，李建峰2，王海波1,2，樊兆民1. 热凝脑膜中动脉对硝酸甘油致偏头痛大鼠三叉神经尾核c-fos基因表达的影响[J]. 山东大学耳鼻喉眼学报, 2010, 24(01): 61-64.
[15] 李海英1 ，张榕1 ，林熹1 ，张文敏2 ，王英歌1 ，林少莲1
. 纯化内耳P0蛋白诱发听神经
脱髓鞘的实验研究[J]. 山东大学耳鼻喉眼学报, 2009, 23(2): 38-41 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
网站版权所有 © 2018 《山东大学耳鼻喉眼学报》编辑部　
地址：山东省济南市山大南路27号（250100）电话：+86-0531-88366912 E-mail: ebhxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发