山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (6): 36-38.doi: 10.6040/j.issn.1673-3770.0.2015.202

• 论著 • 上一篇    下一篇

肺癌肿瘤抑制因子-1在喉鳞癌组织中的表达及其意义

李富1, 赵书佑2   

  1. 1. 包头医学院第一附属医院耳鼻咽喉科, 内蒙古 包头 014010;
    2. 青岛市立医院耳鼻喉头颈外科, 山东 青岛 266011
  • 收稿日期:2015-05-25 出版日期:2015-12-16 发布日期:2015-12-16
  • 通讯作者: 赵书佑. E-mail:13606420458m0@sina.cn E-mail:13606420458m0@sina.cn
  • 作者简介:李富. E-mail:lifu5088@126.com

Expression of tumor suppressor in lung cancer 1 in laryngeal squamous cell carcinoma and its significance

LI Fu1, ZHAO Shuyou2   

  1. 1. Department of Otolaryngology, First Affiliated Hospital of Baotou Medical College, Baotou 014010, Inner Mongolia, China;
    2. Department of Otolaryngology & Head and Neck Surgery, Qingdao Municipal Hospital, Qingdao 266011, Shandong, China
  • Received:2015-05-25 Online:2015-12-16 Published:2015-12-16

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摘要： 目的 研究肺癌肿瘤抑制因子-1(TSLC1)在喉鳞癌组织和癌旁正常组织的表达,探讨TSLC1与喉鳞癌的相关性,以期为喉癌的病因、诊断及治疗提供理论依据.方法 取喉鳞状细胞癌组织标本蜡块45例,其中8例有淋巴结转移.取20例癌旁正常组织标本蜡块作为对照.应用免疫组化PV-6000二步法测定二者中TSLC1的表达,分析TSLC1的表达水平与各临床病理参数的关系.结果用SPSS 10.0统计软件进行统计学分析.结果 TSLC1在喉鳞癌标本中的阳性表达率为17/45(37.8%),明显低于癌旁正常组织中TSLC1的阳性表达率15/20(75.0%),二者比较差异有统计学意义(χ2=7.68, P<0.01).TSLC1的表达下降与喉鳞癌的临床分期、分化程度、淋巴结转移情况差异有统计学意义(χ2=4.15、6.11、4.11, P<0.05),而与年龄、发病部位及性别差异无统计学意义(χ2=0.25、0.09、1.0, P>0.05).结论 TSLC1在喉鳞癌中表达下降,且与喉鳞癌的临床分期、分化程度、淋巴结转移情况有相关性.TSLC1在喉鳞癌中的表达下降可能与喉鳞癌的发生和侵袭有关,但需扩大样本进一步研究.

关键词: 喉鳞状细胞癌, 免疫组织化学, 肺癌肿瘤抑制因子-1

Abstract: Objective To investigate the correlation of tumor suppressor in lung cancer 1 (TSLC1) and laryngeal squamous cell carcinoma, in order to provide a theoretic basis of oncogenesis, diagnosis and treatment of laryngeal cancer. Methods Paraffin-embedded specimens were collected from 45 cases of laryngeal squamous cell carcinoma, 8 of which had lymphatic metastasis. Another 20 specimens from normal adjacent tissues served as controls. The expression of TSLC1 was detected with immunohistochemical staining (PV-6000). The relation between TSLC1 expression and other clinicopathological parameters was analyzed with SPSS 10.0. Results The positive expression of TSLC1 was 17/45 (37.8%) in specimens of laryngeal squamous cell carcinoma, which was significantly lower than in specimens of normal adjacent tissues (15/20, 75%), (χ2=7.68, P<0.01). Loss of TSLC1 expression was correlated with the clinical stage, tumor differentiation and lymph node metastasis (χ2=4.15, 6.11, 4.11, P<0.05), but not correlated with patients' age, gender, or location of primary laryngeal carcinoma (χ2= 0.25, 0.09, 1.0, P>0.05). Conclusion Loss of TSLC1 expression is frequently observed in laryngeal squamous cell carcinoma, which is significantly correlated to the clinical stage, tumor differentiation and lymph node metastasis. The down-regulation of TSLC1 expression may correlate with the occurrence and invasion of laryngeal squamous cell carcinoma, but more studies are needed to verify the hypothesis.

Key words: Laryngeal carcinoma, Tumor suppressor in lung cancer 1, Immunohistochemistry

中图分类号: 

  • R739.65
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