山东大学耳鼻喉眼学报 ›› 2025, Vol. 39 ›› Issue (3): 51-60.doi: 10.6040/j.issn.1673-3770.0.2023.294

• 上海市第六人民医院耳鼻咽喉头颈外科献礼“六院120周年”纪念专题 • 上一篇    

单细胞测序分析小鼠耳蜗螺旋神经节与内外毛细胞间的细胞信号通路

董文琪,于栋祯   

  1. 上海交通大学医学院附属第六人民医院 耳鼻咽喉头颈外科, 上海 200233
  • 发布日期:2025-06-04
  • 通讯作者: 于栋祯. E-mail:doctor.yu@126.com

Single-cell RNA sequencing reveals cellular communication between spiral ganglion neurons and inner and outer hair cells in postnatal mice

DONG Wenqi, YU Dongzhen   

  1. Department of Otorhinolaryngology & Head and Neck Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China
  • Published:2025-06-04

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摘要： 目的 研究在耳蜗听力形成期和成熟期小鼠耳蜗的细胞群以及调控螺旋神经节细胞和内外毛细胞间信号通路表达的配受体。 方法 通过收集公共数据库小鼠耳蜗单细胞测序数据,并利用CellChat软件研究内外毛细胞与临近细胞潜在的细胞与细胞间相互作用。 结果 Seurat分析显示小鼠耳蜗具有15群主要的细胞类型,分别是成纤维细胞、Hensen细胞、鼓膜边界细胞、Coch+/Spp1+成纤维细胞、内外沟细胞、内侧指状细胞/内侧边界细胞、外毛细胞、Reissner膜、巨噬细胞、梭形细胞/根细胞、螺旋神经节、中间细胞、施万细胞/卫星胶质细胞、内毛细胞、红细胞。CellChat通讯分析揭示出P14小鼠耳蜗内外毛细胞和螺旋神经节间的通讯关系主要由Bdnf/Ntrk2信号通路介导,P28时期主要由Ggf6/(Bmpr1a+Bmpr2)、Bmp6/(Bmpr1a+Bmpr2)、Ntf3/Ntrk3、Ntf3/Ntrk2以及Spp1/Cd44、Spp1/(Itgav+Itgb1)、Pdgfa/Pdgfra、Gas6/Tyro3信号通路介导。 结论 本研究基于单细胞转录组数据,初步识别出在耳蜗听力形成期和成熟期螺旋神经节细胞和内外毛细胞间特异性的配受体。

关键词: 单细胞测序, 螺旋神经节, 毛细胞, 细胞间通讯

Abstract: Objective To investigate the cell populations of cochlea during hearing formation and maturation phases and the ligand receptors that regulating the expression of signaling pathways between spiral ganglion neuron and inner and outer hair cells. Methods We investigated the potential cell-cell interactions between inner and outer hair cells and adjacent cells by collecting single-cell sequencing data from mouse cochlea and using CellChat software. Results Seurat analysis showed 15 significant groups of cell types in the mouse cochlea, including Fibroblast, Hensen's cell, Tympanic Border Cell, Coch+/Spp1+ Fibroblast, Inner/Outer Sulcus Cell, Inner Phalangeal Cell/Inner Border Cell, Outer Hair Cell, Reissner's Membrane, Macrophage, Spindle Cell/Root Cell, Spiral Ganglion Neuron, Intermediate Cell, Schwann Cell/Satellite Glial Cell, Inner Hair Cell and Red Blood Cell. Through the CellChat analysis, we observed that the communication relationship between inner and outer hair cells and spiral ganglion neurons in the P14 mouse cochlea was mainly mediated by the Bdnf/Ntrk2 signaling pathway, and Gdf6/(Bmpr1a+Bmpr2), Bmp6/(Bmpr1a+Bmpr2), Ntf3/Ntrk3, Ntf3/Ntrk2, Spp1/Cd44, Spp1/(Itgav+Itgb1), Pdgfa/Pdgfra, Gas6/Tyro3 signaling pathways during P28 period. Conclusion This study initially identified specific ligand receptors between spiral ganglion neuron and inner and outer hair cells during the cochlear hearing formation and cochlea maturation phases based on single-cell transcriptome data.

Key words: Single-cell RNA sequencing, Spiral ganglion neurons, Hair cells, Intercellular communication networks

中图分类号: 

  • R764
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