IL-17对鼻息肉中巨噬细胞炎性蛋白-3α的影响

山东大学耳鼻喉眼学报 ›› 2011, Vol. 25 ›› Issue (2): 37-40.

IL-17对鼻息肉中巨噬细胞炎性蛋白-3α的影响

张敏，夏立军

山西医科大学第一医院耳鼻咽喉-头颈外科，太原 030001

收稿日期:2011-01-07 修回日期:2011-03-08 出版日期:2011-04-16 发布日期:2011-04-16
通讯作者: 夏立军。Email:xlj0671@126.com
作者简介:张敏（1982- ），女，硕士研究生，主要从事耳鼻喉疾病的临床研究。Email:zm200197@126.com

Effects of macrophage inflammatory protein-3α/CCL20 production by interleukin-17 in nasal polyp epithelial cells

ZHANG Min, XIA Li-jun

Department of Otolaryngology & Head and Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan 030001, China

Received:2011-01-07 Revised:2011-03-08 Online:2011-04-16 Published:2011-04-16

摘要/Abstract

摘要：

目的探讨白介素17(IL17)对鼻息肉上皮细胞中巨噬细胞炎性蛋白-3α(macrophage inflammatory protein -3α, MIP-3α/CCL20)的作用。方法离体培养人鼻息肉上皮细胞，不加刺激因子且用IL-17刺激细胞后，用酶联免疫吸附法（ELISA）测定上清液中MIP3α/CCL20的含量，并用逆转录聚合酶链反应（RT-PCR）检测人鼻息肉上皮细胞中MIP-3α/CCL20 mRNA含量的变化。结果 IL-17刺激鼻息肉上皮细胞后，上清液中MIP-3α/CCL20的含量较不加刺激因子时增多，且IL-17对MIP-3α/CCL20的诱导作用具有剂量-时间依从性，同时测定鼻息肉上皮细胞中MIP-3α/CCL20 mRNA的相对表达量，结果相似。结论鼻息肉上皮细胞产生MIP-3α/CCL20，而且IL-17对其有诱导作用。MIP-3α/CCL20可能对T细胞和树突状细胞在鼻息肉的聚集中发挥重要的作用。

关键词: 鼻息肉；IL-17； MIP-3α/CCL20

Abstract:

Objective To explore the effects of macrophage inflammatory protein-3α/CCL20 production by interleukin-17 in nasal polyp epithelial cells. Methods Epithelial cell lines,which were established from nasal polyps, were stimulated by culture medium, IL-17.The amount of MIP-3α/CCL20 in the supernatants was measured by enzyme-linked immunosorbent assay (ELISA). MIP-3α/CCL20 mRNA expression was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Results Expression levels of MIP-3α/CCL20 in nasal polyps were significantly increased compared with those only in the culture medium. IL-17 induced MIP-3α/CCL20 production by nasal polyp epithelial cells in a dose- and time-dependent manner. Conclusions Nasal polyp epithelial cells, by producing MIP-3α/CCL20, which can be induced by Interleukin-17, may play an important role in the recruitment of T cells and DCs in nasal polyps.

Key words: Nasal polyps; IL-17; MIP-3α/CCL20

中图分类号:

R765.25

张敏，夏立军. IL-17对鼻息肉中巨噬细胞炎性蛋白-3α的影响[J]. 山东大学耳鼻喉眼学报, 2011, 25(2): 37-40.

ZHANG Min, XIA Li-jun . Effects of macrophage inflammatory protein-3α/CCL20 production by interleukin-17 in nasal polyp epithelial cells[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(2): 37-40.

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