JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (6): 31-35.doi: 10.6040/j.issn.1673-3770.0.2015.307

Previous Articles     Next Articles

Relationship between the expression of EGFL7 in nasopharyngeal carcinoma and the invasion and metastasis of tumor

ZHAO Yanlian1, LU Yongtian2, YANG Jihong1, ZHANG Juan1, MIAO Fangfang1, LI Jieping1   

  1. 1. Guangzhou Medical University, Guangzhou 511436, Guangdong, China;
    2. Department of Otolaryngology, the Second People's Hospital of Shenzhen, Shenzhen 518005, Guangdong, China
  • Received:2015-07-26 Online:2015-12-16 Published:2015-12-16

PDF (PC)

247

Abstract: Objective To study the expression of epidermal growth factor-like domain 7(EGFL7) in nasopharyngeal carcinoma(NPC), and to investigate its correlation with microvessel density(MVD) and lymphatic vessel density(LVD) and its relationship with the invasion and metastasis of NPC and its possible mechanism. Methods The immunohisto chemical method was used to detect EGFL7 expression of 40 cases of nasopharyngeal carcinoma and 20 cases of nasopharyngitis; MVD and LVD were detected by antibody CD31 and D2-40 antibody for immunohistochemistry in the experimental and control groups, and to analyze the correlation of EGFL7 expression and tumor grade and MVD and LVD. Results The experimental group and the control group were of EGFL7 expression, but in the experimental group EGFL7 expression rate was higher than that of control group (P<0.05); EGFL7 expression and MVD was positively correlated in the experimental group, but LVD did not; in the experimental group, lymph node metastasis of LVD was significantly higher than those without lymph node metastasis of (P<0.05); in the experimental group, EGFL7 expression had no correlation with patient gender, age and EB virus antibody (P>0.05) but had some correlation with clinical stage and lymph node metastasis(P<0.05). Conclusion EGFL7 has no dual function on blood vessels and lymphatic vessels, it only expresses in endothelial cells and nasopharyngeal carcinoma cells. EGFL7 is closely associated with the growth and invasion and metastasis of nasopharyngeal carcinoma (NPC), it may become one of the markers reacting nasopharyngeal carcinoma metastasis and prognosis.

Key words: Nasopharyngeal carcinoma, Epidermal growth factor-like domain 7, VEGF family, Lymphatic vessel density, Micro vessel density

CLC Number: 

  • R739.63
[1] 李骥,王胜资,王纾宜,等.鼻咽癌淋巴管和微血管同期检测与预后[J].临床耳鼻咽喉头颈外科杂志,2010,24(6):253-256. LI Ji, WANG Shengzi, WANG Shuyi, et al. Prognostic value of synchronized detection of microvascular and lymphvessel density in nasopharyngeal carcinoma[J]. J Clin Otorhinolaryngol Head Neck Surgery, 24(6):253-256.
[2] Saad R S, Lindner J, Liu Y, et al. Lymphatic vessel density as prognostic marker in esophageal ade2 nocarcinoma[J]. Am J Clin Pathol, 2009, 131(1):92-98.
[3] 桑娜,王宽松,李景和.Egfl7与肿瘤研究新进展[J].现代生物医学进展,2009,9(23):4556-4559. SANG Na, WANG Kuansong, LI Jinghe. EGFL7 and advances in carcinoma research[J]. Prog Mod Biomed, 2009, 9(23):4556-4559.
[4] Schmidt M, De maziere A, Smyczek T, et al. The role of Egfl7 in vascular morphogenesis[J]. Novartis Found Symp, 2007, 283(1):18-28.
[5] 李京佳,林相国,许涛,等.VEGF家族及其在肿瘤生长中作用的研究[J].现代生物医学进展,2012,12(4):777-780. LI Jingjia, LIN Xiangguo, XU Tao, et al. VEGF and the role of VEGF in tumor growth[J]. Prog Mod Biomed, 2012, 12(4):777-780.
[6] 李文光,张娟,陈永平,等.鼻咽癌VEGF-C和Flt-4的表达与淋巴管生成及淋巴结转移的相关性[J].中国耳鼻咽喉颅底外科杂志,2013,19(3):196-203. LI Wenguang, ZHANG Juan, CHEN Yongping, et al. Expression of VEGF-C and its receptor Flt-4 in nasopharyngeal carcinoma and their relationship with lymph node metastasis[J]. Chin J Otorhinolaryngol Skull Base Surgery, 2013, 19(3):196-203.
[7] 王益民,吴奎,诸禹平,等.EGFL7和VEGF在肾透明细胞癌血管生成及转移中的相关性研究及临床意义[J].临床泌尿外科杂志, 2011,26(10):732-736. WANG Yimin, WU Kui, ZHU Yuping, et al. The correlative research of EGFL7, VEGF Expression in clear cell renal cell carcinoma with angiogenesis and metastasis[J]. J Clin Urol, 2013, 19(3):196-203.
[8] 高永超,杨新明,李仕晟,等.EGFL7在喉癌组织中的表达及临床意义[J].临床耳鼻咽喉头颈外科杂志,2013,27(3):147-150. GAO Yongchao, YANG Xinming, LI Shisheng, et al. The study on clinical significance of the expression of EGFL7 in laryngeal squamous cell carcinoma[J]. J Clin Otorhinolaryngol Head Neck Surgery, 2013, 27(3):147-150.
[9] Chatni S S, Thankappan K. Lymph node status cannot be ignored in survival analysis of laryngeal cancer[J]. Arch Otolaryngol Head Neck Surg, 2008, 134(7):786-787.
[10] 王晓侠,姚小宝,嵇宪生,等.EGFL7基因过度表达与喉癌分化、转移及预后的关系[J].华西医学,2011,26(5):710-713. WANG Xiaoxia, YAO Xiaobao, JI Xiansheng, et al. Association of EGFL7 mRNA overexpression with differentiation, metastasis and prognosis of laryngocarcinoma[J]. West China Med J, 2011, 26(5):710-713.
[11] 王启广,曹友德,刘琼,等. EGFL7在胃癌组织中的表达研究[J].湖南师范大学学报,2013,3(1):53-56. WANG Qiguang, CAO Youde, LIU Qiong, et al. The expression of EGFL7 in gastric carcinoma[J]. J Hunan Normal Univ (Med Sci), 2013, 3(1):53-56.
[12] 刘志勇,欧阳忠,邹小明.EGFL7基因沉默对裸鼠胃癌血管生成的影响[J].贵阳医学院学报,2010,35(3):259-264. LIU Zhiyong, OUYANG Zhong, ZOU Xiaoming. Effect of epidermal growth factor-like domain 7 gene silencing on angiogenesis of stomach carcinoma in nude mice[J]. J Guiyang Med Coll, 2010, 35(3):259-264.
[13] 刘志勇,欧阳忠,邹小明.EGFL7基因沉默对裸鼠乳腺癌血管生成的影响[J]. 国际免疫学杂志,2010, 33(6):476-480. LIU Zhiyong, OUYANG Zhong, ZOU Xiaoming. Effect of RNA interference targeting at EGFL7 on angiogenesis of breast carcinoma in nude mice[J]. Inter J Immunol, 2010, 33(6):476-480.
[14] 刘凤娟,张春玲.非小细胞肺癌Egfl7表达与肿瘤转移及预后的关系[J].齐鲁医学杂志,2012,27(3):104-106. LIU Fengjuan, ZHANG Chunling. Relationship between expression of egfl7 and both metastatis and prognosis in non-small-cell lung cancer[J]. Med J Qilu, 2012, 27(3):104-106.
[15] 李学军,黄纯海,李萃,等.EGFL7在人脑胶质瘤中的表达及其意义[J].肿瘤防治研究,2011,38(2):148-152. LI Xuejun, HUANG Chunhai, LI Cui, et al. Expression of EGFL7 in human gliomas and its correlation with glioma grade[J]. Cancer Res Prev Treat, 2011, 38(2):148-152.
[16] Huang C H, Li X J, Zhou Y Z, et al. Expression and clinical significance of EGFL7 in malignant glioma[J]. J Cancer Res Clin Oncol, 2010, 136(11):1737-1743.
[1] ZHANG Caixia, LIU Yangyun, JIANG Wen, LIU Gengxun, CAO Hang, CHEN Qiong, ZHANG Jishuai. Investigation of chemosensitization induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 72-78.
[2] ZHOU Yibo, GONG Xiaorong, YU Feng. Effects of miR-150 targeted to Nanog on proliferation and invasion of nasopharyngeal carcinoma side population cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 79-84.
[3] DU Zhihong. Application of oro-nasal combination approach under electronic nasopharyngoscope in the nasopharyngeal biopsy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 117-118.
[4] . Effects of concurrent chemotherapy and radiotherapy on the quality of life in children and adolescents with nasopharyngeal carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(2): 55-58.
[5] . Construction of c-Met target chimeric antigen receptor T cells and evaluation the role on nasopharyngeal carcinoma cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(2): 49-54.
[6] . Efficacy of recombinant human epidermal growth factor in prevention of radioactive oral mucosa inflammation and acute radiation dermatitis for nasopharyngeal carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 95-99.
[7] WANG Yaning, GENG Bo, LI Baiyan, CUI Chaoyang, WANG Qirong. The treatment experience on osteoradionecrosis of skull base with nasopharyngeal carcinoma after radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 42-45.
[8] LIU Ye, SUN Dianshui, YIN Jinjun, XIA Ming, XU Ying, SUN Jianhua. Vestibular function damage mechanisms in nasopharyngeal carcinoma patients after intensity-modulated radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 14-17.
[9] WANG Junying, LI Xiaofeng. Effect of deproteinized extract of calf blood gel eye on the injury of corneal endothelial cells after nasopharyngeal carcinoma radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 76-79.
[10] CHE Juan, ZHAO Hongchun, LI Jinling, WANG Yanfei. The relationship between D-Dimer and nasopharyngeal carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(5): 47-49.
[11] ZHANG Xiao-peng1, BIAN Feng2, WANG Li-xin2. Expressions and relativity of COX-2 and EGFR in nasopharyngeal carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 23-27.
[12] SHI Da-zhi1, LI Jian-ming2, LUO Zhi-qiang1, LUO Qin1, JING Qian-cheng1. Clinical analysis of nasopharyngeal carcinoma in pregnancy [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 28-30.
[13] ZHANG Zhong-hua1, WANG Xue-hai2, CAI Xiao-lan3. Expression and significance of p-PTEN， p-Akt and p-FoxO1 in nasopharyngeal carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 55-57.
[14] YANG Jian-ming. Relationship between radioactive otitis media and changes in cross section area of tensor veli palatine (TVP) muscle after radiotherapy in patients with nasopharyngeal carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(1): 31-33.
[15] XU Yuan-yuan, ZENG Quan, HONG Su-ling, HU Guo-hua. Comparison of neoadjuvant chemotherapy followed by concurrent chemoradiotherapy versus concurrent  chemoradiotherapy alone in locoregionally advanced nasopharyngeal carcinoma: A meta analysis. [J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(5): 8-14.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd