Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (2): 111-114.doi: 10.6040/j.issn.1673-3770.0.2017.442

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Effect of beta elemene on the expression of IL-1 beta and ICAM-1 in the retina of diabetic rats

LI Baohua, LIU Ping, WANG Xin   

  1. Department of Ophthalmology, Zhengzhou Peoples Hospital, Zhengzhou 450003, Henan, China
  • Published:2019-03-28

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Abstract: Objective To study the effect of beta elemene on IL-1 beta and intercellular adhesion molecule 1(ICAM-1)expression levels in rats with diabetic retinopathy, to validate the potential of beta elemene in the prevention and treatment of diabetic retinopathy. Methods Forty adult SD healthy rats were randomly divided into four groups: normal control group, diabetic group, 40 mg/kg beta elemene treatment group, and 80 mg/kg beta elemene treatment group. Diabetes was induced in rats by intraperitoneal injection of streptozotocin(STZ). The rats in the beta elemene treatment group were treated with beta elemene solution for 12 weeks. They were then executed, and their retinal tissue, aqueous humor, and serum were extracted. ELISA was used to determine the IL-1 beta and ICAM-1 protein expression in retina, aqueous humor, and serum of rats. Immunohistochemistry was used to detect the expression levels of IL-1 beta and ICAM-1 proteins in retinal tissues of rats. Western blotting was done to evaluate the expression levels of IL-1 beta and ICAM-1 proteins in the retinal tissue. Results ELISA assay revealed that, compared with the normal control group, the levels of IL-1 beta and ICAM-1 proteins in diabetic rat serum and retina were significantly higher(P<0.05); however, 40 mg/kg treatment group and 80 mg/kg treatment exhibited significantly lower IL-1 beta and ICAM-1 levels in rat serum and retina compared to the diabetic group (P<0.05), among which 80 mg/kg beta elemene treatment group exhibited lower serum and retinal IL-1 beta and ICAM-1 protein levels compared to 40 mg/kg beta elemene treatment group (P<0.05). There was no significant difference in IL-1 beta and ICAM-1 protein levels in aqueous humor among the groups (P>0.05). Immunohistochemistry results showed that compared with the normal control group, retinal IL-1 beta protein levels were higher in the diabetic group; however, 40 mg/kg treatment group and 80 mg/kg treatment group exhibited lower IL-1 beta levels compared with diabetic group; the retinal tissue isolated from normal group exhibited almost no expression of ICAM-1 protein, whereas the diabetic group exhibited high expression of ICAM-1 protein. The 40 mg/kg treatment group and the 80 mg/kg treatment group exhibited lower expression levels of ICAM-1 compared to diabetic group. Western blotting revealed that, compared with the normal control group, diabetes group exhibited significantly higher expression levels of retinal IL-1 beta and ICAM-1 proteins (P<0.05); 40 mg/kg treatment group and 80 mg/kg treatment group exhibited significantly lower retinal IL-1 beta and ICAM-1 protein levels compared to diabetic group (P<0.05), among which 80 mg/kg treatment group exhibited significantly lower retinal IL-1 beta and ICAM-1 protein levels compared to 40 mg/kg treatment group (P<0.05). Conclusion Beta elemene can inhibit the production of IL-1 beta and ICAM-1 inflammatory factors in serum and retinal tissue of diabetic rats, suggesting that beta elemene may be used in the prevention and treatment of diabetic retinopathy.

Key words: Beta elemene, Diabetic retinopathy, IL-1 beta, Intercellular adhesion molecule 1

CLC Number: 

  • R774
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