Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (5): 69-72.doi: 10.6040/j.issn.1673-3770.0.2018.425

• Original Article • Previous Articles     Next Articles

Expression and biological function of long chain non-coding cytoskeleton regulator RNA in nasopharyngeal carcinoma

Lin LI1,Heng ZHANG2,Yufei XIAN3,Xiaoli SHENG3()   

  1. 1. Department of Otorhinolaryngology Head and Neck Surgery, Zhuhai Hospital of Guangdong Provincial People's Hospital，Jinwan Central Hospital, Zhuhai 519040, Guangdong, China
    2. Department of Pathology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510080, Guangdong, China
    3. Department of Otorhinolaryngology Head and Neck Surgery, Guangdong Academy of Medical Sciences, Guangdong Province People's Hospital, Guangzhou 510080, Guangdong, China
  • Received:2018-09-26 Revised:2018-11-08 Online:2019-09-20 Published:2019-10-15
  • Contact: Xiaoli SHENG E-mail:shengzhang2016@126.com

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Abstract: Objective

To investigate the expression and biological function of long non-coding cytoskeleton regulator RNA (CYTOR) in nasopharyngeal carcinoma.

Methods

Real-time PCR was used to examine CYTOR expression in nasopharyngeal carcinoma tissues (n = 32), nasopharyngeal carcinoma cell lines (CNE1 and HONE1), 28 patients with benign nasopharyngeal lesions, and human nasopharyngeal epithelial cells (NP69). Moreover, CYTOR expression in the CNE1 and HONE1 cell lines was knocked down using RNAi assay. MTT, Transwell, and wound healing assays were also used to detect the proliferation and migration of nasopharyngeal carcinoma cells from CNE1 and HONE1 after downregulating CYTOR expression.

 Results

CYTOR expression in the nasopharyngeal carcinoma tissues and nasopharyngeal carcinoma cell lines was observed to be significantly higher than that in the nasopharyngeal benign lesion mucosal tissues and NP69. After transient inhibition of CYTOR expression, the migration and proliferation abilities of the nasopharyngeal carcinoma cell lines were also observed to decrease significantly (P < 0.05).

 Conclusion

CYTOR expression is elevated in pharyngeal cancer tissues and nasopharyngeal carcinoma cell lines and is positively correlated with the migration ability of nasopharyngeal carcinoma.

Key words: cytoskeleton regulator RNA, Nasopharyngeal Carcinoma, Invasion, Metastasis

CLC Number: 

  • R739.6

Table 1

Downregulation of CYTOR expression inhibits nasopharyngeal carcinoma cell migration"

时间 组别 宽度 F P
0 h 0.22 0.81
CNE1 406.32±22.11
CNE1_SiNC 411.36±19.21
CNE1_SiCYTOR 414.21±26.06
24 h 46.69 <0.001
CNE1 102.11±26.21
CNE1_SiNC 116.65±30.14
CNE1_SiCYTOR 336.45±45.69

Fig. 1

Effect of the inhibition of CYTOR expression on nasopharyngeal carcinoma cell invasion"

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