JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2009, Vol. 23 ›› Issue (2): 1-4 .

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Effect of endothelin 1 on mitosis of human HEP 2 cells in vitro

XU Dingyuan1, ZHENG Xuelian2, WU Bo3, XUE Fei1, LI Zeqinq1, JI Junfeng1, WANG Qiuping1   

  1. (1. Department of Otorhinolaryngology, Nanjing General Hospital of Nanjing Military Command, Chinese PLA, Nanjing 210002, China; 2. Molecular and Translational Medicine Lab, Second Affiliated Hospital of Sichuan University, Chengdu 610041, China;
    3.Department of Pathology, Nanjing General Hospital of Nanjing Military Command, Chinese PLA, Nanjing 210002, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-04-16 Published:2009-04-16
  • Contact: WANG Qiuping

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Abstract:

To investigate the effect of endothelin 1 on mitosis of HEP 2 cells. MethodsExpression of endothelin receptors on HEP 2 cells was determined by RTPCR and immunohistochemical methods. HEP 2 cell lines were exposed to different concentrations of the endothelin 1, and mitosis of HEP 2 cells was measured by 3ResultsGene expression of the endothelinreceptor was detected by RTPCR. Immunohistochemical studies showed that endothelin receptor immunoreactivity appeared in HEP 2 cells. Addition of endothelin 1 resulted in an increase of DNA synthesis in concentration dependence. ConclusionEndothelin 1 can promote mitosis of HEP 2 cells and may play an important role in the progression of laryngeal carcinoma.

 

Key words: Endothelin 1; HEP 2 cells; Mitosis

CLC Number: 

  • R76719
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