JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2009, Vol. 23 ›› Issue (5): 4-7.

• Articles • Previous Articles     Next Articles

Effect of siRNA by targeting the human hTERT gene on morphology in Hep-2 cells

 TANG Zhi, CHEN Yong-Ming, WANG Ji-Qun   

  1. 1. Department of Otolaryngology, First People′s Hospital of ShundeCity, Shunde 528300, Guangdong, China;
    2. Mecical College of Jinan University, Guangzhou 510632, China
  • Received:2009-04-27 Revised:2009-09-11 Online:2009-10-16 Published:2009-10-16

PDF (PC)

1047

Abstract:

Objective  To   investigate the effect of the small interfering RNA (siRNA) targeting hTERT gene in Hep-2 cells on morphology. Methods  siRNA targeting site 1718 of hTERT mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was observed by phase contrast microscopy and transmission electron microscopy. Results   48 hours after siRNA were transfected into Hep2 cells, numerous round cells and shrinkage of the cellular membrane were observed under phase contrast microscopy, cells showed innocence transformation under HE stain microscopy, and apoptosis and oncosis appeared under transmission electron microscopy. Conclusion  siRNA can promote the Hep-2 cell to show innocence transformation and apoptosis.

Key words: Laryngeal neoplasms, siRNA, Gene therapy

CLC Number: 

  • R739.63
[1] FU Yugui, WANG Hailan, SUN Dechong, YANG Peizhen, CHEN Yanlin, CHEN Zhipeng. Total laryngectomy with partial preservation of the cricoid cartilage ring. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 73-75.
[2] LI Langen, WEI Wei, ZHANG Yufeng, Geriletu, YANG Jia, ZHANG Yanmei. The experiment of SIRT1 on against oxidative stress to retinal pigmented epithelium cells [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(6): 56-59.
[3] YAN Yu, ZHU Jiang. Advances in the diagnosis of early laryngeal carcinoma and precancerous laryngeal lesions. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(4): 80-85.
[4] DU Xiangge, ZHANG Yingchun, YAN Xin, WANG Cui, ZHAO Bojun. In vitro study of the effect of Casein Kinase2 siRNA on anginogesis [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(3): 76-80.
[5] WEI Xi-jian1, YIN Rui-lan2, GUO Xing3. Expression of a proliferation-inducing ligand in laryngeal squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(1): 14-16.
[6] LU Guo-wei， WANG Lin, SUN Yan， LI Wei， HUA Hui， GE Rui-feng. Detection of different subtypes of HPV DNA in 57 cases of laryngeal carcinoma in easter of the shandong province [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(6): 16-19.
[7] LI Da-wei, DONG Pin, GAO Shang. Significance of apoptotic and proliferative indices and  P-glycoprotein expression in laryngeal squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(4): 8-12.
[8] CHEN Jian-qiu1,2, ZHU Min-hui1, QIU Xiao-xia1, XIA Si-wen1,YE Ben2, CHEN Shi-cai1, ZHENG Hong-liang1. Expressions and clinical significances of osteopontin and p53 in laryngeal squamous cell carcinoma tissues [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(2): 19-22.
[9] LI Zhi-zhang1, LI Qin2, GENG Yu-yan3， SHAO Yang1. Effect and significance of the TGF-β1/Smad4 signaling system in laryngeal squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(2): 16-.
[10] LI Da-wei, GAO Shang, CHEN Xin-wei, WANG Guo, DONG Pin. Expression and significance of hypoxia-inducible factor 1α and p-glycoprotein in laryngeal squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2010, 24(4): 6-9.
[11] LIU Zhang, SUN Yan, HUA Hui, CHEN Zhijun, LI Wei, SUN Wenhai. Clinical features of laryngeal carcinoma in females in the east of Shandong Province [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2010, 24(2): 8-10.
[12] ZHANG Zhong-Hua, LIANG Kun, MA Chao, LEI Da-Peng, PAN Xin-Liang. Expressions and clinical significance of osteopontin and uPA in squamous cell carcinoma of the larynx [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(6): 491-494.
[13] ZHU Chun-sheng,CHEN Jian-qiu . Apoptotic effect of human maligant melanoma cell line A375 induced by tumor-specific recombinant TRAIL gene [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(5): 408-411 .
[14] WANG Lin,SUN Yan,CHEN Zhi-jun,LI Wei,SUN Wen-hai . Clinical features and management of Methicillin-resistant Staphylococcus Aureus (MRSA) infection after laryngectomy [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(2): 127-129 .
[15] ZHANG Han-bing,ZHANG Dao-gong,PAN Xin-liang,LUAN Xin-yong,XIE Guang . STAT3 expression in laryngeal carcinoma tissues and its relation with Survivin expression [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(2): 123-126 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd