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    16 October 2009 Volume 23 Issue 5
      
    Articles
    Effect of siRNA by targeting the human hTERT gene on morphology in Hep-2 cells
    TANG Zhi, CHEN Yong-Ming, WANG Ji-Qun
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  4-7. 
    Abstract ( 1300 )   PDF (528KB) ( 1049 )   Save
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    Objective  To   investigate the effect of the small interfering RNA (siRNA) targeting hTERT gene in Hep-2 cells on morphology. Methods  siRNA targeting site 1718 of hTERT mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was observed by phase contrast microscopy and transmission electron microscopy. Results   48 hours after siRNA were transfected into Hep2 cells, numerous round cells and shrinkage of the cellular membrane were observed under phase contrast microscopy, cells showed innocence transformation under HE stain microscopy, and apoptosis and oncosis appeared under transmission electron microscopy. Conclusion  siRNA can promote the Hep-2 cell to show innocence transformation and apoptosis.

    Modified cochlear view in cochlear implantation
    MA Hou-sheng1, ZHENG Shi=cai1, DONG Zhen-ling1, WANG Xiang-wen2
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  8-9. 
    Abstract ( 1679 )   PDF (263KB) ( 1225 )   Save
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    Objective  To evaluate the modified cochlear view so as to access the appearance and accurate insertion depth of cochlear implantation.   Methods  21 patients, 30 days to 4 years of age, had both Xu′s X-ray pictures and modified X-ray pictures taken. The number of electrodes in the cochlear canal was counted, and then the number of electrodes by X-ray picture and by operation were statistically dealt with by a t-test. ResultsInserted artificial electrodes could be discerned on both the cochlear view and the modified cochlear view. There was no difference among the data from operation and plan radiographs. Conclusion  The modified cochlear view is simple and comfortable for patients younger than 4 years, and can show the precise appearance and accurate insertion depth of the cochlear implantation.

    Particle repositioning maneuver for benign paroxysmal positional  vertigo
    CAO Xiao-Ping, WANG Tian-Sheng, GU Dong-Sheng, TUN Chun-Ping
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  10-12. 
    Abstract ( 1647 )   PDF (264KB) ( 1034 )   Save
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    Objective   To investigate the therapeutic efficacy of the Canalith repositioning maneuver for benign paroxysmal positional vertigo(BPPV). Methods  The Epley maneuver, Semont maneuver and Barbecue rotation were applied to 32 case of BPPV.  Results  After treatment of manipulative reduction, positional vertigo disappeared completely in 27 cases(84.4%), and in  3(9.4%) the therapy was effective. In  2 cases(6.3%) the therapy had no effect, but after receiving  physical therapy, they were cured. Conclusions  For benign paroxysmal positional vertigo, the Canalith repositioning maneuver is recommended, and the benefits of physical therapy are demonstrated.

    Comparison of serum ECP levels between chronic rhinosinusitis patients with and without nasal polyps
    SUN Ling-li1,2, LU Han-qiang1, ZHU Lu-ping1, QI Qin-hong3, CHENG Lei1
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  25-27. 
    Abstract ( 1434 )   PDF (247KB) ( 1076 )   Save
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    Objective  To compare the serum levels of eosinophil cationic protein (ECP) between chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) and without nasal polyps (CRSsNP). Methods  Serum levels of ECP were investigated by using the  ImmunoCAP100E system in patients with CRSwNP (n=99) and CRSsNP (n=59), and in healthy controls (n=84). Results  Significantly different serum levels of ECP were observed among the CRSwNP, CRSsNP, and control groups (P<0.05). Intergroup analysis showed that the serum levels of ECP were significantly higher in patients with CRSwNP than in the controls (P<0.01), especially in the moderatetosevere group compared with the control group (P<0.01). There was no significant difference between the CRSwNP and CRSsNP groups (P>0.05), as well as between the mild and moderatetosevere patients in the CRSwNP and CRSsNP groups(P>0.05). Conclusion  Eosinophilic inflammation may play a role in the pathogenesis of CRSwNP and CRSsNP, however, there is no relationship between disease severity and ECP levels in the serum.

    Coronal computed tomographic analysis of the uncinate process and frontal sinus drainage pathway
    WANG Liyin, WANG Zhenhai
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  28-30. 
    Abstract ( 2288 )   PDF (299KB) ( 1465 )   Save
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     Objective  To recognize the regularity of the uncinate process'superior attachment sites and frontal sinus drainage pathway in rhino-patients. Methods  50 coronal computed tomography scans of the sinuses performed from July 2007 to November 2007 were evaluated. Result  About 62% (59/95) of the uncinate process had two or more superior attachment sites, and the other 38%(36/95) had one superior attachment site.When the uncinate process had two or more superior attachment sites, 95%(56/59) of frontal sinuses directly drained into the middle nasal meatus. Conclusion  The structure of the frontal recess could be clearly recognized by coronal computed tomography.

    Heterogeneous acellular dermal matrix in repairing mucosa defect of the nasal septum
    ZHAO Li-min, ZHANG Qing-quan, SUN Yan, SONG Xi-cheng, ZHANG Hua, CHEN Xiu-mei
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  31. 
    Abstract ( 1491 )   PDF (241KB) ( 1431 )   Save
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    Objective  To study the clinical effect of the heterogeneity (cattle) acellular deunal matrix in repairing mucosa defect of the nasal septum. Methods  Twenty-seven cases of mucosa defect or perforation of the nasal septum were treated with the heterogeneity acellular deunal matrix after surgery on the nasal septum. Results  During a follow-up of 4 weeks to 2 years, twenty-four patients who had undergone nasal septal reconstruction or tumor resection had no perforation, and three patients with nasal septal perforation were successfully repaired. Conclusion  The heterogeneity acellular deunal matrix can be easily obtained and it is a new method to repair mucosa defect of the nasal septum.

    Lamellar sectional anatomy of endoscopic sphenoidotomy
    ZHAO Zhen-hua1, WANG Qi-rong1,LIU Shu-wei2, HAN Fei1, GU Lin-tao1, LIANG Hui1, CUI Zhao-yang1, YU Shu-dong1
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  33-36. 
    Abstract ( 1437 )   PDF (423KB) ( 988 )   Save
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    Objective   To provide lamellar sectional anatomy data for endoscopic sphenoidotomy. Methods  The digital freezing milling technique was used to obtain the continuous sectional data as thin as 0.1mm of 15 cadaver heads(30sides) without organic lesion. The  sphenoid sinus and some surrounding structures were observed and measured on typical and continuous cross sectional image. Results  46.7% of optical nerve canal and 70% of internal carotid bulged on the area of the lateral sphenoid sinus. The maximal vertical longitude of the sphenoid sinus was (3.41±0.56) mm and the maximal horizontal longitude was (2.24±0.35)mm. The distance of the aperture of the sphenoid sinus to the internal carotid was (19.36±2.26)mm, and the distance of the aperture to the optic nerve canal was (8.83±1.42)mm. Conclusion  The lamellar sectional image obtained from the digital freezing milling technique offers a better understanding of complex anatomical structures and provides sectional anatomy data for sphenoidotomy.

    Effect  of  sublingual  immunotherapy  with  dust  mites  guttae  in  allergic  rhinitis
    ZHANG Ke-ke, YE Ping, YANG Yan, SHI Li
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  37-38. 
    Abstract ( 1967 )   PDF (205KB) ( 1220 )   Save
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    Objective  To survey the effect and safety of sublingual immunotherapy with dust mites in allergic rhinitis. Methods  54 patients with allergic rhinitis were treated with sublingual immunotherapy for 1 year. The symptom scores pre- and post-immunotherapy and side effects were recorded. Results  There were significant differences between pre-and post-therapy (P<0.01). Conclusion  Sublingual immunotherapy with dust mites guttae has obvious curative effect on allergic rhinitis.

    Effect of different endoscopic surgical methods for nasal septum deviation
    TANG Chen, LIU Shou-hou, Lv Ling-yun
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  39. 
    Abstract ( 1817 )   PDF (221KB) ( 1518 )   Save
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    Objective  To investigate the therapeutic effects of different endoscopic surgical methods for nasal septum deviation. Methods  A total of 212 patients with deviation of the nasal septum were enrolled in this study.Four kinds of endoscopic surgical methods were employed. Results  There were 52 cases tht had submucosal resection, 37 cases had modified sub-mucosal resection, 58 cases had rectification surgery and 65 cases had septorhinoplast. Efficacy and complications were not significantly different among the four groups. Conclusion  The experience and technique of the operator and the instrument can influence the selection and therapeutic effect of endoscopic surgical methods for nasal septum deviation.

    Tonsillectomy and adenoidectomy in treatment of child sinusitis
    GAO Ben-xi,WANG Yang,YANG Feng-shan
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  41-42. 
    Abstract ( 2233 )   PDF (222KB) ( 1075 )   Save
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    Objective  To observe the effect of tonsillectomy or adenoidectomy in the treatment of sinusitis. Methods  63 children with sinusitis were treated with tonsillectomy or adenoidectomy, and then were given anti-inflammatory treatment for three days, sprayed three times per day with nano-silver antimicrobial gel and took azithromycin particles once per day, lasting for 10 days. Results  One month later, nasal obstruction, purulent snivel, dizziness, headache, snoring and other symptoms of 61 cases disappeared, while two cases of deviation of the nasal septum had a slight feeling of nasal obstruction, and snoring was improved. Conclusion  Tonsillectomy or adenoidectomy is effective for those unaffected by drugs and at the same time it is effective for those with snoring.

    Nasal tracheal casing lien postoperatively applied in the root of the tongue
    SUN Feng-qian, CAO Gao-xue
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  48-49. 
    Abstract ( 1523 )   PDF (181KB) ( 1036 )   Save
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    Objective  To prevent the anhelation caused by hydronacus of the root of the tongue and glossoccomn after operation on the root of the tongue. Methods  After tracheal intubation by the nasal cavity and general narcosis, an operation was carried out on the root of the tongue and the raw surface was oversown. After the operation, the tracheal cannula was kept for 48 to 72 hours. Wintermin(100?mg) and phernargan(100?mg) in 5%GS(500?mL) were given to keep the patients in a state of hibernation. Results  Choking was effectively avoided and secretions of the lower respiration tract were cleaned up in time. Of the 10 patients, none died of airway obstruction. Conclusions  This method can effectively prevent anhelation caused by hydronacus after operation on the root of the tongue.

    Causes and followup of patients with hoarseness caused by  diseases from larynxs
    YU De-xian
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  50-51. 
    Abstract ( 1324 )   PDF (206KB) ( 1231 )   Save
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    Objective  To improve knowledge about the diseases from the larynx which cause paralysis of laryngeal nerves so that the diseases could be diagnosed and treated early. Methods  46 cases of hoarseness were tested by a fibrolaryngoscope and CT scan of the larynx. There were no visible diseases in their larynxes except for vocal fold paralysis, and the following test included Xray picture of the chest and esophageal barium meal perspective. The cases when no diseases were found were followed up each month. Results28 cases had been diagnosed as having tumors from larynxes when they visited doctors the first time, and 8 were diagnosed during their following-up period. The positive rate was 78.26% (36/46). Conclusion   If patients with hoarseness had only a sign of laryngeal nerve paralysis, in the other words, there were no visible diseases in their larynxes, the tumors from the larynxes should be considered, and a CT scan of the larynx, X-ray picture of the chest and esophageal barium meal perspective should be used as routine tests. It is very important to follow up on cases when no diseases were found when they finished all of the above tests.

    Beveldown phaco chop in phacoemulsification
    ZHENG -Yan1,2,ZHU Zhao-rong2
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  57-60. 
    Abstract ( 1677 )   PDF (264KB) ( 1294 )   Save
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    Objective  To evaluate the outcomes of bevel-down phaco-chop and study the mechanism which results in the loss of corneal endothelium in phacoemulsification.  Methods  251 eyes accepting phacoemulsification were divided into two groups. The technique of bevel-down phaco-chop was performed on 127 eyes in group A. The nucleus density grade was Ⅱ in 28,  Ⅲ in 69, and Ⅳ-Ⅴin 30 eyes. The other 124 eyes were divided into group B and on which the traditional technique of bevel-up phaco-chop was performed. The nucleus density grade wasⅡin 31,  Ⅲ in 65, and Ⅳ-Ⅴin 28 eyes. The differences of accumulated energy complex(AEC) and corneal endothelium loss (CEL) over 6 months postoperation were compared between the 2 groups.  Results  In group A,  the AEC was 2.7±0.5, 11.9±2.1 and 30.2±8.1 seconds; the CEL was (5.1±0.6)%, (8.9±0.4)%  and (12.5±1.6)% in eyes with grade Ⅱ, Ⅲ and Ⅳ-Ⅴnucleus density respectively. In group B, the AEC was 2.6±0.4, 12.2±2.5 and 31.7±8.9;  the CEL was (5.0±0.5)%, (11.2±0.8)% and (20.6±1.4)% respectively. The CEL had no difference in the eyes with grade Ⅱ nucleus density(P>0.05), but had differences  in the eyes with grade Ⅲ and Ⅳ-Ⅴnucleus densities between the 2 groups(P<0.05).  Conclusion  Bevel-down phaco-chop can reduce damage to corneal endothelial cells and is more useful in hard cataract phacoemulsification.

    Prognosis of phacoemulsification in patients with dry eyes
    CHEN Min, CAI Li-mei, CAI Fu-xu, WU Hui-ying
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  61-63. 
    Abstract ( 1419 )   PDF (277KB) ( 1130 )   Save
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    Objective  To compare the tear film function and complication between phacoemulsification (Phaco) and extracapsular cataract extraction (ECCE) in patients with dry eyes. Methods  38 patients(45 eyes) were in the Phaco group and 28 patients (30 eyes) were in the ECCE group. The tear film function, complications and final visual outcomes were analyzed before and after operation. Results  Of the 66 patients, 56 had age-related dry eyes and 10 had secondary Sjgren's syndrome. 60 eyes had predominant aqueous deficiency (Schirmer I score of 5.0?mm or less), and 15 had Schirmer scores between 6.0?mm and 9.0?mm.  The tearfilm breakup time was 5 seconds or less in 63 eyes and 6-9 seconds in 12 eyes. Postoperatively, 32 eyes had superficial punctate keratopathy and 25 had an epithelial defect. The final visual acuity was 1.0 in 40 eyes, 0.8 to 0.5 in 22 eyes, and 0.4 to 0.1 in 13 eyes. All the differences between the two groups had statistical significance(P<0.05). Conclusions  Phaco is safer and causes fewer complications in treatment of age-related dry eyes on patients with or without associated systemic disorders than ECCE.

    Analysis value of computerized visualfield examination during treatment of anterior ischemic optic neuropathy
    LI Hai-yan, LU Xiu-zhen, LIU Li
    JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2009, 23(5):  64. 
    Abstract ( 1386 )   PDF (238KB) ( 1298 )   Save
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    Objective  To explore the analysis value of computerized visual-field examination during treatment of anterior ischemic optic neuropathy (AION).  Methods  60 patients (84 eyes) with AION were observed. Visual acuity and visual field were measured with logarithmic visual acuity chart and perimeter before and after therapy. Results  After 1 month of medication, 24 cases(28.6%)  were cured , and the therapy was obviously effective in 16 (19.0%) , effective in 8(9.5%), and  ineffective in 36(43.0%). The visual acuity of 24 among these ineffective 36 cases was not increased, but the percentage of the visual field defect decreased by more than 40%. The symptoms of the patients were relieved. The visual sensitivity was elevated in the defect area of the visual field. The mean sensitivity was obviously improved, and the mean defect and loss variance were decreased significantly. Conclusions  Computerized visual-field examination plays an important role in evaluating treatment effect of AION. The change of perimetry is more explicit than that of visual acuity in treatment evaluation.