Abstract:

Objective   To investigate the biological behavior of nasopharyngeal carcinoma(NPC）cell CNE2 and 5-8F that survived from multiple 5-aminolevulinic acid (5-ALA) mediated PDTs (5-ALA-PDTs). Methods   CNE2 and 5-8F cells were discontinuously treated with median lethal dosage PDT 3 times, surviving cells were collected for culture, then to evaluate the change of proliferation, migration, adhesion and expressions of Ang-1 and Ang-2 in these cells were evaluated. Results   The CNE2 and 5-8F cells that survived from multiple 5-ALA-PDTs showed a lower level of proliferation, migration, adhesion and expressions of Ang-1 and Ang-2（P＜0.05）. Conclusion   Multiple 5-ALA-PDTs could stably decrease the malignant biological behavior of surviving NPC cells, having potential value of reducing lymphatic metastasis.

Key words: Photodynamic therapy; Nasopharyngeal carcinoma; Biologic behaviors;  Angiopoietin