Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2020, Vol. 34 ›› Issue (4): 97-100.doi: 10.6040/j.issn.1673-3770.0.2019.413

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2- aminoethoxydiphenyl borate inhibits inflammatory reactions of neutrophilic nasal polyps in vitroDAI Fei, WEI Jinjin, TANG Xinyue, CHEN Zheng, LIN Lin Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital North of Fudan University, Shanghai 201907, ChinaAbstract:Objective〓

Nasal polyps(NP)are frequently divided into subtypes, including eosinophilic and neutrophilic, among others, according to their endotypes. However, the mechanisms of neutrophilic NP need to be further elucidated. This study explores the influence of 2-aminoethoxydiphenyl borate(2-APB)on inflammatory reactions of neutrophilic NP in vitro. MethodsWe used immunofluorescence and immunohistochemical staining to detect the infiltration of neutrophils(NEU)in normal nasal mucosa and NP tissues. These tissues were subsequently cultured in vitro using an organ culture technique, and concentrations of Orai1 and myeloperoxidase(MPO)were determined using enzyme-linked immunosorbent assay(ELISA). 2-APB was administered to both cultures, and the measurement of Orai1 and MPO concentrations using ELISA was repeated. ResultsAll samples were identified as neutrophilic NP. The number of NEU was higher in NP than in normal nasal mucosa. Concentrations of Orai1 and MPO were also higher in NP cultured in vitro than in normal tissue. After the administration of 2-APB, Orai1 protein and the mediator MPO were reduced significantly in the cultures. Conclusion2-APB may inhibit inflammatory reactions of neutrophilic NP in vitro.   

  1. Keywords: Neutrophils;
    Nasal polyps;
    Nasal mucosa;
    Inflammatory mediator;
    Myeloperoxidase慢性鼻窦炎(chronic rhinosinusitis, CRS)临床常分为两型:慢性鼻窦炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps, CRSsNP)和慢性鼻窦炎伴有鼻息肉(chronic rhinosinusitis with nasal polyps, CRSwNP)［1-2］。CRS在我国的发病率大约为8%［3］, 该病会给患者本人及其家庭都带来繁重的经济负担和削弱的劳动力, 目前针对CRS的治疗已成为国家和社会的沉重负担。
  • Received:2019-08-19 Online:2020-07-20 Published:2020-08-28

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CLC Number: 

  • R765.4
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