Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2022, Vol. 36 ›› Issue (5): 30-34.doi: 10.6040/j.issn.1673-3770.0.2021.125

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GLP-1 protected the diabetic retinopathy through induction of autophagy in rats

SU Jie1, YANG Fuyu1, LI Meng1, CHEN Huiru2, JIANG Lisheng3, WANG Lixiang4   

  1. 1. Department of Ophthalmology, Affiliated hospital of North China University of Science and Technology, Tangshan 063000, He bei, China;
    2. Department of Ophthalmology, Tangshan Nanhu Hospital, Tangshan 063000, Hebei, China;
    3. Department of Ophthalmology, Luanzhou Peoples Hospital, Luanzhou 063700, Hebei, China;
    4. Department of Ophthalmology, Luannan County Hospital, Luannan 063500, Hebei, China
  • Published:2022-09-20

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Abstract: Objective Research the GLP-1 induced autophagy through regulation of mTOR signaling pathways, whether there is a protective role in diabetic retinopathy rats. Methods Conform to the requirements of the diabetic retinopathy rats model, divided into model group, insulin group and GLP-1 group, each group of 6, ALL the groups were measured to realise the fasting blood glucose level in rats after 0 d,4 w, 8 w and 12 w, after 12 w killing the rats, we removed the retinal tissue for HE staining and test the expression of P53, LC3 by immunohistochemical method. Collected blood serum to detect oxidative stress product ROS and MDA content with serum superoxide dismutase(SOD)method. Detected mTOR protein expression with Western blotting method. Results Compared with model group, insulin group and GLP-1 group can obviously reduce fasting blood glucose, the difference was statistically significant, and is no statistically significant difference in the two groups. HE staining showed the retinal ganglion cells of model group disordered arrangement and the cells reduced or missed, but in insulin group and GLP-1 group, retinal ganglion cells arrangement was neat, no significant decline, close to normal. Immunohistochemical display, The LC3 and P53 protein expression respectively(2.34±0.13,0.46±0.03)in GLP-1 group increased significantly compared with other groups, and the differences were statistically significance(P<0.05). Oxidative stress product ROS and MDA content respectively(74.68±4.08,55.60±1.50)in GLP-1 group reduced, compared with other groups, the differences between groups were statistically significant(P<0.05). Compared with other groups, mTOR protein content(0.43±0.04)in GLP-1 group decreased, and the differences between groups were statistically significant(F=105.447, P<0.05). Conclusion Glp-1 may through activation of autophagy by regulating mTOR signaling pathways, then reducing oxidative stress injury of retina, as to protect the retina.

Key words: GLP-1, Autophagy, Oxidative stress reaction, mTOR

CLC Number: 

  • R774
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