JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY)

Previous Articles     Next Articles

Apoptosis  of Hep-2 cells induced  by celecoxib

ZHAO Yong-qiang1,  FENG Hui-wei1, YU Ke-na1, SHI Mei2, FAN Xian-liang1   

  1. 1. Department of Otolaryngology &  Head and Neck Surgery,  Second Hospital of Shandong University, Jinan  250033, Shandong, China; 2. Department of Biology Science, Shandong University, Jinan 250100, Shandong, China
  • Received:2013-12-20 Online:2014-04-16 Published:2014-04-16

Abstract: Objective    To investigate the mechanism of celecoxib, a COX-2 inhibitor, in inducing cell apoptosis in human laryngocarcinoma Hep-2 cells. Methods    MTT was used to observe the proliferation of Hep-2 cells treated with various doses of celecoxib. Cell apoptosis was determined by flow cytometry and morphologic observation under fluorescent microscopy with Hoechst33258 staining. Protein expressions of Bax, Bcl-2, COX-2 were examined  by western blot. Caspase-3 activation was examined  by western blot. Results    MTT results showed that celecoxib could inhibit the proliferation of Hep-2 cells in a time- and concentration- dependent manner. Hoechst33342 fluorescent staining showed the nucleus gradually appeared agglutination and  fragmentation after the treatment of celecoxib. Apoptosis rate in the groups treated with 70 and 100μ mol/L celecoxib  were (28.9±2.74)% and (32.7±3.96)% correspondingly. Wwestern blot showed that celecoxib could down-regulate the levels of expressions of COX-2 and Bcl-2 protein, up-regulate Bax protein’s expression, and activate caspase-3. Conclusion    Celecoxib  can effectively inhibit the proliferation of Hep-2 cells and induce the apoptosis of Hep-2 cells. The induction of apoptosis may be related to the increased Bax/Bcl-2 ratio  and activation of  caspase-3.

Key words: Hep-2 cell, Cyclooxygenase-2, Apoptosis, Celecoxib

CLC Number: 

  • R739.65
[1] SU Jie, AI Xin, MA Chunmei, YANG Fuyu, HUANG Shuai. Relationship between acute intraocular hypertension and lateral geniculate neuron autophagy and apoptosis in rats [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 68-71.
[2] ZHAO Xiaoyan, WU Caiqin, REN Xiaoyong, WANG Zhenghui. A polysaccharide from hedyotis diffusa induces apoptosis and inhibits migration in Hep-2 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(2): 84-87.
[3] ZHANG Caixia, LIU Yangyun, JIANG Wen, LIU Gengxun, CAO Hang, CHEN Qiong, ZHANG Jishuai. Investigation of chemosensitization induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 72-78.
[4] . Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(2): 67-72.
[5] XU Yuanteng, CHEN Ruiqing, LIN Gongbiao, FANG Xiuling, YU Shujuan, LIANG Xiaohua, ZHANG Rong. Effect of silencing PDCD4 gene on proliferation of Hep-2 cells and the expression of β-catenin by RNA interference technique. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(5): 110-114.
[6] LI Qin, CHEN Yanlin, MA Yanyi, ZHANG Yongdong. Effects of interferon gamma on Eosinophil apoptosis and Eotaxin levels in nasal lavage fluid of allergic rhinitis rats. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 30-33.
[7] ZHANG Xiao-peng1, BIAN Feng2, WANG Li-xin2. Expressions and relativity of COX-2 and EGFR in nasopharyngeal carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 23-27.
[8] QIN Jie-sheng1, WANG Hui-ge1, LIN Xin-qiang1, HONG Liang-li2. Expression and significance of IL-15mRNA in nasal polyps [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 34-37.
[9] ZHUANG Xiao-yan1,2, LI Qin2, CHEN Yan-lin2, MA Yan-yi2. Effects of budesonide on eosinophil apoptosis in nasal lavage fluid of allergic rhinitis rats [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 68-71.
[10] ZHANG Shu-jia1, LI Pei-hua2, JIA Wen-bo1, XU Heng-guang1, BAO Feng-xiang1, LI Li1. 1. Expression and clinical significance of programmed cell death 5 (PDCD5) in laryngeal squamous cell carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 23-26.
[11] TIAN Tian-jie1, GENG Yang1, YE Qing1,2, ZHANG Xian-zeng3, XIE Shu-sen3, SHEN Fan1. Apoptosis and the injury of the inner ear [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 35-38.
[12] LI Da-wei, DONG Pin, GAO Shang. Significance of apoptotic and proliferative indices and  P-glycoprotein expression in laryngeal squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(4): 8-12.
[13] ZHANG Yang. Apoptosis and expression of  bcl-2 and bax in inner ear immune response [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(3): 34-36.
[14] ZHOU Bin1, XU Ping1, LI Yan1, MA Xiao-nan1, WU Shuo1, XIA Hong2. Protection of CEP-1347 on noise-damaged cochlea [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2010, 24(4): 25-28.
[15] ZHU Chun-sheng,CHEN Jian-qiu . Apoptotic effect of human maligant melanoma cell line A375 induced by tumor-specific recombinant TRAIL gene [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(5): 408-411 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!