Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2023, Vol. 37 ›› Issue (5): 128-134.doi: 10.6040/j.issn.1673-3770.0.2022.455

• Original Article • Previous Articles     Next Articles

The protective effect of hydroxysafflor yellow A on light-induced retinal injury in rats

FU Yixuan1, WANG Tao2, CAO Jing3, LI Guangda2, ZHENG Lingfang4, XU Wanjing5, ZHAO Shuang6   

  1. 1. Linyi People's Hospital Postgraduate Training Base, Jinzhou Medical University, Linyi 276000, Shandong, China2. Department of Ophthalmology, Linyi People's Hospital, Linyi 276000, Shandong, China3. Department of Medicine, Linyi People's Hospital, Linyi 276000, Shandong, China4. Clinical Medical College, Weifang Medical University, Weifang 261000, Shandong, China5. Qingdao Medical College, Qingdao University, Qingdao 266000, Shandong, China6. Department of Graduate, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250000, Shandong, China
  • Published:2023-10-13

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Abstract: Objective To investigate the protective effect of hydroxysafflor yellow A(HSYA)on light-induced retinal injury in rats. Methods Thirty Sprague-Dawley rats were randomly divided into the blank control group, model control group, and HSYA group(10 rats per group). The rats in the latter two groups were treated with 0.9% NaCl solution and 5.0 mg/mL HSYA, respectively, once a day for 14 days. At the end of the experiment, the pathological sections of the right eye were removed and examined. The morphology of the retina was observed after HE staining, the expression of active caspase-3 protein was detected by immunohistochemistry, and the apoptotic index was calculated using TUNEL staining. Results HE staining showed that the retinal tissue morphology and cell structure of the blank control group were normal. In the model control group, the retinal tissue morphology was disordered, the cells of the RGC, INL, and ONL layers were unevenly dispersed, a large number of cells were disintegrated, the boundary between the inner and outer segments of the photoreceptor cells was not clear, and the retinal pigment epithelium(RPE)layer was disordered. The morphology of the retina in the HSYA group was generally normal, and no obvious damage was observed. The integrated optical density of caspase-3 protein detected by immunohistochemistry was significantly higher in the model control group than in the blank control group(P<0.001), while the integrated optical density of caspase-3 protein in the HSYA group was significantly lower than in the model control group(P<0.001). TUNEL staining showed that the apoptotic index of the model control group was significantly higher than that of the blank control group(P<0.001), while the apoptotic index of the HSYA group was significantly lower than that of the model control group(P=0.021), and the differences were statistically significant. Conclusion HSYA improved abnormal changes in the retinal tissue structure caused by light damage in rats, reduce the expression of caspase-3 protein, and inhibit the apoptosis of retinal cells after light damage. Based on these findings, HSYA has a certain protective effect against retinal light damage.

Key words: Light-induced retinal injury, Hydroxysafflor yellow A, Caspase-3 protein, Apoptosis

CLC Number: 

  • R774.1
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