Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2023, Vol. 37 ›› Issue (5): 128-134.doi: 10.6040/j.issn.1673-3770.0.2022.455

• Original Article • Previous Articles     Next Articles

The protective effect of hydroxysafflor yellow A on light-induced retinal injury in rats

FU Yixuan1, WANG Tao2, CAO Jing3, LI Guangda2, ZHENG Lingfang4, XU Wanjing5, ZHAO Shuang6   

  1. 1. Linyi People's Hospital Postgraduate Training Base, Jinzhou Medical University, Linyi 276000, Shandong, China2. Department of Ophthalmology, Linyi People's Hospital, Linyi 276000, Shandong, China3. Department of Medicine, Linyi People's Hospital, Linyi 276000, Shandong, China4. Clinical Medical College, Weifang Medical University, Weifang 261000, Shandong, China5. Qingdao Medical College, Qingdao University, Qingdao 266000, Shandong, China6. Department of Graduate, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250000, Shandong, China
  • Published:2023-10-13

PDF (PC)

62

Abstract: Objective To investigate the protective effect of hydroxysafflor yellow A(HSYA)on light-induced retinal injury in rats. Methods Thirty Sprague-Dawley rats were randomly divided into the blank control group, model control group, and HSYA group(10 rats per group). The rats in the latter two groups were treated with 0.9% NaCl solution and 5.0 mg/mL HSYA, respectively, once a day for 14 days. At the end of the experiment, the pathological sections of the right eye were removed and examined. The morphology of the retina was observed after HE staining, the expression of active caspase-3 protein was detected by immunohistochemistry, and the apoptotic index was calculated using TUNEL staining. Results HE staining showed that the retinal tissue morphology and cell structure of the blank control group were normal. In the model control group, the retinal tissue morphology was disordered, the cells of the RGC, INL, and ONL layers were unevenly dispersed, a large number of cells were disintegrated, the boundary between the inner and outer segments of the photoreceptor cells was not clear, and the retinal pigment epithelium(RPE)layer was disordered. The morphology of the retina in the HSYA group was generally normal, and no obvious damage was observed. The integrated optical density of caspase-3 protein detected by immunohistochemistry was significantly higher in the model control group than in the blank control group(P<0.001), while the integrated optical density of caspase-3 protein in the HSYA group was significantly lower than in the model control group(P<0.001). TUNEL staining showed that the apoptotic index of the model control group was significantly higher than that of the blank control group(P<0.001), while the apoptotic index of the HSYA group was significantly lower than that of the model control group(P=0.021), and the differences were statistically significant. Conclusion HSYA improved abnormal changes in the retinal tissue structure caused by light damage in rats, reduce the expression of caspase-3 protein, and inhibit the apoptosis of retinal cells after light damage. Based on these findings, HSYA has a certain protective effect against retinal light damage.

Key words: Light-induced retinal injury, Hydroxysafflor yellow A, Caspase-3 protein, Apoptosis

CLC Number: 

  • R774.1
[1] Zhao Y, Shen Y. Light-induced retinal ganglion cell damage and the relevant mechanisms[J]. Cell Mol Neurobiol, 2020, 40(8): 1243-1252. doi:10.1007/s10571-020-00819-0
[2] 徐恩沛, 孙先勇. 渗出性年龄相关性黄斑变性并发视网膜色素上皮脱离的诊治进展[J]. 山东大学耳鼻喉眼学报, 2023, 37(3): 125-142. doi:10.6040/j.issn.1673-3770.0.2022.052 XU Enpei, SUN Xianyong. Diagnosis and treatment of exudative age-related macular degeneration combined with retinal pigment epithelial detachment[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(3): 125-142. doi:10.6040/j.issn.1673-3770.0.2022.052
[3] 杨凯转, 徐延山, 南莉. 视网膜光化学损伤机制及组织学变化的研究进展[J]. 中华临床医师杂志(电子版), 2013, 7(4): 1703-1705. doi:10.3877/cma.j.issn.1674-0785.2013.04.061 YANG Kaizhuan, XU Yanshan, NAN Li. Research progress on the mechanism and histological changes of retinal photochemical injury[J]. Chinese Journal of Clinicians(Electronic Edition), 2013, 7(4): 1703-1705. doi:10.3877/cma.j.issn.1674-0785.2013.04.061
[4] Li L, Zhou YF, Li YL, et al. Aqueous extract of Cortex Dictamni protects H9c2 cardiomyocytes from hypoxia/reoxygenation-induced oxidative stress and apoptosis by PI3K/Akt signaling pathway[J]. Biomedecine Pharmacother, 2017, 89: 233-244. doi:10.1016/j.biopha.2017.02.013
[5] Lv YN, Fu LS. The potential mechanism for Hydroxysafflor yellow A attenuating blood-brain barrier dysfunction via tight junction signaling pathways excavated by an integrated serial affinity chromatography and shotgun proteomics analysis approach[J]. Neurochem Int, 2018, 112: 38-48. doi:10.1016/j.neuint.2017.10.012
[6] Ye J, Wang R, Wang M, et al. Hydroxysafflor yellow A ameliorates myocardial ischemia/reperfusion injury by suppressing calcium overload and apoptosis[J]. Oxid Med Cell Longev, 2021, 2021: 6643615. doi:10.1155/2021/6643615
[7] Yu L, Jin Z, Li M, et al. Protective potential of hydroxysafflor yellow A in cerebral ischemia and reperfusion injury: An overview of evidence from experimental studies[J]. Front Pharmacol, 2022, 13: 1063035. doi:10.3389/fphar.2022.1063035
[8] Zhang Z, Huo Y, Zhou Z, et al. Hydroxysafflor yellow A(HSYA)protects endplate chondrocytes against IL-1β-Induced injury through promoting autophagy[J]. Evid Based Complement Alternat Med, 2022: 6326677. doi:10.1155/2022/6326677
[9] Xie ZG, Wu XW, Gong YY, et al. Intraperitoneal injection of Ginkgo biloba extract enhances antioxidation ability of retina and protects photoreceptors after light-induced retinal damage in rats[J]. Curr Eye Res, 2007, 32(5): 471-479. doi:10.1080/02713680701257621
[10] Contín MA, Benedetto MM, Quinteros-Quintana ML, et al. Light pollution: the possible consequences of excessive illumination on retina[J]. Eye, 2016, 30(2): 255-263. doi:10.1038/eye.2015.221
[11] 高园园, 黄洁, 俞洋. 视网膜光损伤动物模型的研究进展[J]. 牡丹江医学院学报, 2020, 41(4): 133-135. doi: 10.13799/j.cnki.mdjyxyxb.2020.04.036 GAO Yuanyuan, HUANG Jie, YU Yang. Research progress of animal model of retinal light injury[J]. Journal of Mudanjiang Medical University, 2020, 41(4): 133-135. doi:10.13799/j.cnki.mdjyxyxb.2020.04.036
[12] 梁五林, 张明倩, 崔爽, 等. 红花保护心血管系统的药理作用和临床应用研究进展[J]. 中医药学报, 2022, 50(6): 94-102. doi:10.19664/j.cnki.1002-2392.220140 LIANG Wulin, ZHANG Mingqian, CUI Shuang, et al. Research progress on pharmacological effects and clinical applications of Carthamus tinctorius L. in protecting cardiovascular system[J]. Acta Chinese Medicine and Pharmacology, 2022, 50(6): 94-102. doi:10.19664/j.cnki.1002-2392.220140
[13] Chen HY, Zhou HF, Yang JH, et al. Guhong injection mitigates myocardial ischemia/reperfusion injury by activating GST P to inhibit ASK1-JNK/p38 pathway[J]. Phytomedicine, 2023, 109: 154603. doi:10.1016/j.phymed.2022.154603
[14] Yuan ML, Zhang Y, Wang L, et al. Study on the mechanism of Tong-Qiao-Huo-Xue Decoction regulating apoptosis via ASK1/MKK4/JNK pathway in MCAO/R rats[J]. Phytomedicine, 2022, 106: 154437. doi:10.1016/j.phymed.2022.154437
[15] Lv YN, Qian YS, Ou-Yang AJ, et al. Hydroxysafflor yellow A attenuates neuron damage by suppressing the lipopolysaccharide-induced TLR4 pathway in activated microglial cells[J]. Cell Mol Neurobiol, 2016, 36(8): 1241-1256. doi:10.1007/s10571-015-0322-3
[16] Liu L, Si N, Ma YC, et al. Hydroxysafflor-yellow A induces human gastric carcinoma BGC-823 cell apoptosis by activating peroxisome proliferator-activated receptor gamma(PPARγ)[J]. Med Sci Monit, 2018, 24: 803-811. doi:10.12659/msm.905587
[17] Xu YY, Lee J, Park YD, et al. Molecular dynamics simulation integrating the inhibition kinetics of hydroxysafflor yellow A on α-glucosidase[J]. J Biomol Struct Dyn, 2018, 36(4): 830-840. doi:10.1080/07391102.2017.1300544
[18] Sun ZH, Wang YY, Xu R, et al. Hydroxysafflor yellow A improved retinopathy via Nrf2/HO-1 pathway in rats[J]. Open Life Sci, 2022, 17(1): 284-292. doi:10.1515/biol-2022-0030
[19] 许静, 蔡小军. 羟基红花黄色素A抗晶状体氧化损伤的实验研究[J]. 眼科新进展, 2008, 28(3): 190-194. doi:10.13389/j.cnki.rao.2008.03.024 XU Jing, CAI Xiaojun. Experimental study on HSYA preventing lens from oxidative stress[J]. Recent Advances in Ophthalmology, 2008, 28(3): 190-194. doi:10.13389/j.cnki.rao.2008.03.024
[20] 黄沁园, 黄敏丽, 何纯刚. 羟基红花黄色素A对高糖作用下视网膜微血管内皮细胞增殖的影响[J]. 国际眼科杂志, 2011, 11(8): 1324-1326. doi:10.3969/j.issn.1672-5123.2011.08.005 HUANG Qinyuan, HUANG Minli, HE Chungang. Effects of hydroxysafflor yellow A on high glucose-induced proliferation of retinal capillary endothelial cells[J]. International Journal of Ophthalmology, 2011, 11(8): 1324-1326. doi:10.3969/j.issn.1672-5123.2011.08.005
[21] 甄栋钦, 梁金凤, 王海英, 等. 红花黄色素滴眼液对大鼠光化学损伤后视网膜变性的保护作用[J]. 国际医药卫生导报, 2014, 20(18): 2770-2774. doi: 10.3760/cma.j.issn.1007-1245.2014.18.002 ZHEN Dongyin, LIANG Jinfeng, WANG haiying, et al. Protective effect safflor yellow eye drops on rats’ retina with photochemical damage[J]. International Medical and Health Guide News, 2014, 20(18): 2770-2774. doi: 10.3760/cma.j.issn.1007-1245.2014.18.002
[22] 龚哲, 张晓旎, 李红红, 等. 羟基红花黄素A对脑缺血再灌注后缺血半暗带自噬活性的调节作用[J]. 中国病理生理杂志, 2017, 33(3): 449-454. doi:10.3969/j.issn.1000-4718.2017.03.011 GONG Zhe, ZHANG Xiaoni, LI Honghong, et al. Modulation of autophagy in ischemic penumbra by hydroxysafflor yellow A after cerebral ischemia/reperfusion injury[J]. Chinese Journal of Pathophysiology, 2017, 33(3): 449-454. doi:10.3969/j.issn.1000-4718.2017.03.011
[23] 王鸿梅. 羟基红花黄色素A对心肌梗死大鼠游离脂肪酸、一氧化氮及一氧化氮合酶的影响[J]. 齐鲁药事, 2009, 28(10): 625-627. doi:10.3969/j.issn.1672-7738.2009.10.024 WANG Hongmei. Effects of hydroxysafflor yellow A on FFA, NO and iNOS in rats of myocardial infarction[J]. Qilu Pharmaceutical Affairs, 2009, 28(10): 625-627. doi:10.3969/j.issn.1672-7738.2009.10.024
[24] 刘科峰, 黄海玲, 朱明琼, 等. 乌司他丁对光损伤大鼠视网膜的保护作用[J]. 中国临床研究, 2016, 29(12): 1601-1605. doi:10.13429/j.cnki.cjcr.2016.12.003 LIU Kefeng, HUANG Hailing, ZHU Mingqiong, et al. Protective effect of ulinastatin on light-induced retinal injury in rats[J]. Chinese Journal of Clinical Research, 2016, 29(12): 1601-1605. doi:10.13429/j.cnki.cjcr.2016.12.003
[25] Zhang X, Luo T, Mou YR, et al. Morphological and electrophysiological changes of retina after different light damage in three patients: three case reports[J]. World J Clin Cases, 2022, 10(30): 11128-11138. doi:10.12998/wjcc.v10.i30.11128
[26] 张文静. 水通道蛋白4基因敲除对视网膜光损伤的影响[D]. 南京:南京医科大学, 2009
[27] Yang P M, Cheng K C, Huang J Y, et al. Sulforaphane inhibits blue light-induced inflammation and apoptosis by upregulating the SIRT1/PGC-1α/Nrf2 pathway and autophagy in retinal pigment epithelial cells[J]. Toxicol Appl Pharmacol, 2021, 421: 115545. doi:10.1016/j.taap.2021.115545
[28] 蔡丽, 周炼红. Caspase与视网膜细胞凋亡的研究进展[J]. 临床眼科杂志, 2016, 24(4): 380-383 CAI Li, ZHOU Lianhong. Current progress in understanding of caspase's role in retinal cell apoptosis[J]. Journal of Clinical Ophthalmology, 2016, 24(4): 380-383
[29] Sk S, Majumder A, Sow P, et al. Exploring a new family of designer copper(II)complexes of anthracene-appended polyfunctional organic assembly displaying potential anticancer activity via cytochrome c mediated mitochondrial apoptotic pathway[J]. J Inorg Biochem, 2023, 243: 112182. doi:10.1016/j.jinorgbio.2023.112182
[30] Lim C, Zhen A X, Ok S, et al. Hesperidin protects SH-SY5Y neuronal cells against high glucose-induced apoptosis via regulation of MAPK signaling[J]. Antioxidants(Basel), 2022, 11(9). doi:10.3390/antiox11091707
[31] 毛德文, 陈月桥, 王丽, 等. Caspase-8及Caspase-3与细胞凋亡[J]. 辽宁中医药大学学报, 2008, 10(10): 148-150. doi:10.13194/j.jlunivtcm.2008.10.150.maodw.095 MAO Dewen, CHEN Yueqiao, WANG Li, et al. Relationship of caspase-8 and caspase-3 to apoptosis[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2008, 10(10): 148-150. doi:10.13194/j.jlunivtcm.2008.10.150.maodw.095
[32] 王萧, 胡世兴, 麦细焕. Caspase-3在急性视网膜光损伤模型中的作用[J]. 中山大学学报(医学科学版), 2003, 24(5): 463-466, 470. doi:10.3321/j.issn: 1672-3554.2003.05.013 WANG Xiao, HU Shixing, MAI Xihuan. The effect of caspase-3 in rat's retinal models of acute light damage[J]. Journal of Sun Yat-sen University(Medical Sciences), 2003, 24(5): 463-466, 470. doi:10.3321/j.issn: 1672-3554.2003.05.013
[1] XIE Yi, HAN Fengchan. Role of miRNAs in inner ear development and apoptosis or regeneration of auditory hair cells [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 126-129.
[2] XU Li, WANG Zhiyi, CHEN Wei, XUE Fei, ZHANG Yong, CHENG You, JI Junfeng, CUI Pengcheng, JIANG Manjie. Effect of MicroRNA-24 on the proliferation, migration, invasion, and apoptosis of LSCC cells [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2018, 32(6): 30-37.
[3] SU Jie, AI Xin, MA Chunmei, YANG Fuyu, HUANG Shuai. Relationship between acute intraocular hypertension and lateral geniculate neuron autophagy and apoptosis in rats [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 68-71.
[4] ZHAO Xiaoyan, WU Caiqin, REN Xiaoyong, WANG Zhenghui. A polysaccharide from hedyotis diffusa induces apoptosis and inhibits migration in Hep-2 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(2): 84-87.
[5] ZHANG Caixia, LIU Yangyun, JIANG Wen, LIU Gengxun, CAO Hang, CHEN Qiong, ZHANG Jishuai. Investigation of chemosensitization induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 72-78.
[6] . Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(2): 67-72.
[7] LI Qin, CHEN Yanlin, MA Yanyi, ZHANG Yongdong. Effects of interferon gamma on Eosinophil apoptosis and Eotaxin levels in nasal lavage fluid of allergic rhinitis rats. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 30-33.
[8] QIN Jie-sheng1, WANG Hui-ge1, LIN Xin-qiang1, HONG Liang-li2. Expression and significance of IL-15mRNA in nasal polyps [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 34-37.
[9] ZHAO Yong-qiang1, FENG Hui-wei1, YU Ke-na1, SHI Mei2, FAN Xian-liang1. Apoptosis  of Hep-2 cells induced  by celecoxib [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 41-45.
[10] ZHUANG Xiao-yan1,2, LI Qin2, CHEN Yan-lin2, MA Yan-yi2. Effects of budesonide on eosinophil apoptosis in nasal lavage fluid of allergic rhinitis rats [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 68-71.
[11] ZHANG Shu-jia1, LI Pei-hua2, JIA Wen-bo1, XU Heng-guang1, BAO Feng-xiang1, LI Li1. 1. Expression and clinical significance of programmed cell death 5 (PDCD5) in laryngeal squamous cell carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 23-26.
[12] TIAN Tian-jie1， GENG Yang1， YE Qing1,2， ZHANG Xian-zeng3， XIE Shu-sen3， SHEN Fan1. Apoptosis and the injury of the inner ear [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 35-38.
[13] LI Da-wei, DONG Pin, GAO Shang. Significance of apoptotic and proliferative indices and  P-glycoprotein expression in laryngeal squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(4): 8-12.
[14] ZHANG Yang. Apoptosis and expression of  bcl-2 and bax in inner ear immune response [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(3): 34-36.
[15] ZHOU Bin1, XU Ping1, LI Yan1， MA Xiao-nan1， WU Shuo1, XIA Hong2. Protection of CEP-1347 on noise-damaged cochlea [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2010, 24(4): 25-28.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd