Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2025, Vol. 39 ›› Issue (4): 26-30.doi: 10.6040/j.issn.1673-3770.0.2024.367

• Clinical Research • Previous Articles     Next Articles

Expression of EphB2 in nasopharyngeal carcinoma tissues and its correlation with clinicopathological characteristics

HUANG Qiao, REN Yi, HOU Tao, LIAO Xingwei, ZHU Zi’ang, ZHAN Xiaolin, LIU Ying, YIN Shihua   

  1. Department of Otorhinolaryngology & Head and Neck Surgery, the Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, Guangxi, China
  • Online:2025-07-20 Published:2025-08-11

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36

Abstract: Objective To investigate the expression and clinical significance of erythropoietin-producing human hepatocellular B2(EphB2)in tissues of patients with nasopharyngeal carcinoma(NPC). Methods 194 NPC patients were enrolled as the study group, and 80 patients with chronic nasopharyngeal mucosal inflammation were included as the control group. Immunohistochemistry(IHC)and Western blot were used to detect the EphB2 levels in tissues. Kaplan-Meier survival analysis was performed to evaluate the relationship between the expression of EphB2 and the prognosis of patients with NPC, and Cox regression analysis was used to identify independent risk factors for poor prognosis. Results Immunohistochemistry and protein blotting experiments showed that EphB2 protein level was highly expressed in NPC tissues compared with chronic inflammatory tissues of nasopharyngeal mucosa(t=157, P<0.001). The expression level of EphB2 in NPC tissues was correlated with clinical stage(χ2=24.573, P<0.001), depth of tumor invasion(χ2=6.280, P=0.012), presence of cervical lymph node metastasis(χ2=5.576, P=0.018), distant metastasis(χ2=7.588, P=0.006), EBV infection(χ2=6.754, P=0.009), and the presence of recurrence(χ2=9.182, P=0.002). Kaplan-Meier curves revealed that NPC patients with high EphB2 expression had a poor prognosis(χ2=7.010, P=0.008). Cox regression analysis further revealed that EphB2 was an independent risk factor affecting the prognosis of NPC. Conclusion EphB2 is an independent risk factor affecting the prognosis of NPC, which may be involved in the process of NPC occurrence and development, and it is expected to be a biological marker and potential therapeutic target for the early diagnosis and prognosis evaluation of NPC.

Key words: Nasopharyngeal carcinoma, Erythropoietin-producing human hepatocellular B2, Clinical pathology, Immunohistochemistry, Cox regression analysis

CLC Number: 

  • R739.6
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