Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (3): 12-17.doi: 10.6040/j.issn.1673-3770.0.2023.097

• Original Article • Previous Articles    

Expression and clinical significance of KRT4 in laryngeal carcinoma and adjacent normal mucosal tissues

WANG Jingsong1, YU Can1, ZHANG Xi1, DENG Qicheng1, XIE Zhuoliang1, ZHAO Rui1, WEN Bei2, LIU Hai1   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan, China2. Department of Otorhinolaryngology & Head and Neck Surgery, Sichuan Provincial People's Hospital, Chengdu 610031, Sichuan, China
  • Published:2024-06-04

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Abstract: Objective To investigate the relationship between the expression level of keratin 4(KRT4)in laryngeal cancer patients and the clinicopathological characteristics and prognosis of the patients. Methods 70 specimens of laryngeal cancer tissue, 70 specimens of normal mucosa tissue adjacent to cancer, and 40 specimens of vocal cord polyp tissue were collected. Immunohistochemical SP method was used, and the relationship between KRT4 expression and clinical pathological characteristics and prognosis of laryngeal cancer was analyzed statistically. Results The immunohistochemical SP method showed that the expression level of KRT4 in laryngeal cancer tissue was significantly lower than that in adjacent normal mucosal tissue and vocal cord polyp tissue, and the difference was statistically significant(χ2=51.511,P<0.001). There were significant correlationbetween the expression level of KRT4 and the clinical stage(χ2=5.741,P=0.017), degree of differentiation(χ2=6.713,P=0.010), and cervical lymph node metastasis of patients(χ2=4.639,P=0.031). Single factor Log rank test analysis showed that KRT4 expression(χ2=23.962,P<0.001), clinical stage(χ2=4.621,P=0.032), cervical lymph node metastasis(χ2=7.549,P=0.006), and prognosis of laryngeal cancer were correlated. The Kaplan Meier survival curve showed that the survival rate of the KRT4 positive expression group was significantly higher than that of the KRT4 negative expression group. Further research using Cox regression models revealed that KRT4 expression is an independent risk factor affecting the prognosis of laryngeal cancer. Conclusion The expression level of KRT4 in laryngeal cancer tissue is significantly lower than that in normal mucosa tissue and vocal cord polyp tissue adjacent to the cancer, and the expression of KRT4 is related to the clinical stage, differentiation degree, and cervical lymph node metastasis of patients. Laryngeal cancer patients with positive expression of KRT4 have a better prognosis. Therefore, KRT4 is expected to become an important biological indicator to guide the diagnosis, treatment, and prognosis evaluation of laryngeal cancer.

Key words: Laryngeal cancer, Keratin 4, Immunohistochemistry, Cervical lymph node metastasis, Cox regression models, Kaplan-Meier survival curve

CLC Number: 

  • R739.65
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