Abstract: To explore the expression of neuron specific enolaes (NSE), S protein(S100) and glial fibrillary acidic protein(GFAP) in retinoblastoma(Rb) and its clinical significance. MethodThe NSE, S100 and GFAP levels were determined in 32 cases of eyeball excision by immunohistochemistry, and the relation between their expressions and tumor differentiation and clinical stage were analyzed. ResultsThe Rb pathological stage was not identical to the clinical stage. The immunohistochemistry results showed that NSE was positive in both differentiated and undifferentiated Rb, S100 and GFAP were negative in undifferentiated Rb, but S100 and GFAP were positive in nerve fibers and nerve glial cells among the high differentiated Rb. ConclusionsCalcification is one of the features of Rb. Tumor necrosis is associated with its differentiation degree. There are positive NSE neuron cells in Rb, and S100 and GFAP double positive nerve glial cells in the high differentiated Rb.

Key words: Neuron specific enolaes, S protein100, Retinoblastoma, Glial fibrillary acidic protein, Immunohistochemistry