ISSN 1673-3770 CN 37-1437/R
山东大学耳鼻喉眼学报 ›› 2016, Vol. 30 ›› Issue (3): 68-72.doi: 10.6040/j.issn.1673-3770.0.2015.456
张小兵1,戴方平2,侯赟1,Brand-Saberi B2
ZHANG Xiaobing1, DAI Fangping2, HOU Yun1, Brand-Saberi B
摘要： 目的 观察Wnt家族负调节因子cAxin2在鸡胚内耳早期发育中的表达方式。 方法 制备cAxin2的探针,并用地高辛RNA标记盒(Roche)标记。将受精白来亨鸡蛋孵化,按Hamburger和Humilton(1951)标准分期,宰杀HH11-28各期鸡胚并固定,全胚胎原位杂交。挑选合适鸡胚并用震动切片机切片。 结果 鸡的等位基因cAxin2的表达始于HH-14+期发育内耳的耳杯中,并在内耳的形成及进一步耳囊的分化过程中显示了一个动态的表达方式。 结论 cAxin2参与鸡胚内耳发育。
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