42例喉癌患者血清 microRNAlet-7a 水平的变化及意义

doi:10.6040/j.issn.1673-3770.0.2019.468

摘要/Abstract

摘要： 目的

探讨42例喉癌患者血清microRNAlet-7a水平的变化及其对喉癌的诊断价值。

方法

qRT-PCR检测42例喉癌患者（手术前后）及42例健康体检者血清miRNA let-7a的表达，分析血清let-7a水平与喉癌的临床病理因素的关系，用受试者工作曲线（ROC）分析其对喉癌的诊断效能。

结果

血清let-7a在实验组比正常对照组相对表达下降分别为：0.38±0.0831、0.933±0.0723，差异有统计学意义；喉癌患者术前比术后1周血清let-7a的相对表达下降为：0.38±0.0831、0.488±0.1468，差异有统计学意义。血清let-7a的相对表达量与淋巴结转移有关联(P＜0.05)；与年龄、性别、饮酒、吸烟、浸润深度、肿瘤分型、TNM分期、分化程度无关联(P >0.05)。受试者工作曲线（ROC）显示以0.765为血清let-7a相对表达量的最佳临界值诊断喉癌，其灵敏度为76.5%，特异度为76.1%，AUC为0.850，95%置信区间为0.776~0.932。

结论

喉癌患者血清miRNAlet-7a水平较健康者出现下调，let-7a有望成为诊断喉癌的新的分子标志物。血清let-7a水平与淋巴结转移有关，有助于肿瘤病情判断与术后病情监测，但有待进一步研究。

关键词: 喉癌, microRNA, 血清

Abstract: Objective

To investigate changes in the serum let-7a expression in laryngeal squamous cell carcinoma (LSCC) patients and the diagnostic value of let-7a for LSCC.

Methods

Forty-two LSCC patients and 42 healthy subjects who were hospitalized in our hospital were enrolled in this study. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to determine the expression level of let-7a in patient serum samples, the association between serum let-7a levels and clinical and pathological LSCC parameters, and the diagnostic efficacy of let-7a for LSCC.

Results

Relative expression levels of let7a in the experimental (0.380±0.083) and normal control (0.933±0.0723) groups were significantly different . Relative let-7a expression levels in patients with laryngeal cancer before (0.380±0.0831) and after (0.488±0.1468) surgery were also significantly different. The let-7a level in tissues was associated with lymph node metastasis , but not with gender, age, smoking, alcohol consumption, tumor classification, infiltration depth, TNM stage, or degree of differentiation . ROC curve analysis showed that the best critical value for the relative expression of let-7a in 0.765 was laryngeal carcinoma with a sensitivity of 756.0%, a specificity of 76.1%, an AUC of 0.850, and a 95% confidence interval of 0.776-0.932.

Conclusion

Overall, we confirmed that serum let-7a expression is down-regulated in LSCC patients and is a potential molecular marker for laryngeal cancer diagnosis. Our findings also showed that the serum let-7a level is associated with lymph node metastasis, which is helpful for tumor disease judgment and postoperative tumor monitoring, but further research is needed to validate these findings.

Key words: Laryngeal carcinoma, microRNA, Serum

中图分类号:

R767.1

罗露,周恩,欧阳思,陈义,肖旭平,王继华. 42例喉癌患者血清 microRNAlet-7a 水平的变化及意义[J]. 山东大学耳鼻喉眼学报, 2019, 33(5): 96-100.

Lu LUO,En ZHOU,Si OUYANG,Yi CHEN,Xuping XIAO,Jihua WANG. Expression of the microRNA let-7a in the serum of 42 patients with laryngeal cancer and its significance[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(5): 96-100.

图/表 3

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临床病理参数		总例数	let-7a表达量	t	P
性别	男	38	0.381±0.087	0.158	0.876
性别	女	4	0.374±0.042	0.158	0.876
年龄（岁）	≤60	24	0.371±0.079	0.846	0.403
年龄（岁）	＞60	18	0.393±0.089	0.846	0.403
吸烟	吸烟	30	0.372±0.088	0.844	0.403
吸烟	不吸烟	12	0.396±0.069	0.844	0.403
饮酒	饮酒	25	0.377±0.078	0.341	0.735
饮酒	不饮酒	17	0.386±0.092	0.341	0.735
肿瘤分型	声门上型	9	0.371±0.052	0.413	0.682
肿瘤分型	声门型	33	0.384±0.090	0.413	0.682
淋巴结转移	无	35	0.393±0.081	2.250	0.03
淋巴结转移	有	7	0.319±0.070	2.250	0.03
浸润深度	T1~T2	38	0.377±0.084	0.747	0.459
浸润深度	T3~T4	4	0.41±0.084	0.747	0.459
TNM分期	I~II	33	0.388±0.080	1.118	0.27
TNM分期	III~IV	9	0.353±0.093	1.118	0.27
分化程度	中分化	6	0.380±0.088	0.220	0.827
分化程度	高分化	36	0.384±0.029	0.220	0.827