叶酸受体阳性循环肿瘤细胞检测在喉鳞状细胞癌诊断中的应用价值

doi:10.6040/j.issn.1673-3770.0.2022.433

摘要/Abstract

摘要： 目的探讨42例喉鳞状细胞癌(LSCC)患者血清中叶酸受体阳性循环肿瘤细胞(FR⁺-CTCs)的水平及其在LSCC诊断中的应用价值。方法检测42例LSCC患者和22例喉良性病变患者外周血中FR⁺-CTCs水平,分析FR⁺-CTCs与LSCC临床病理因素的关系。用受试者工作曲线(receiver operating characteristic curve, ROC)分析FR⁺-CTCs检测在LSCC诊断中的工作效能。结果 LSCC组FR⁺-CTCs水平高于喉良性病变组,差异具有统计学意义(t=7.269,P<0.001)。血清FR⁺-CTCs水平与肿瘤临床分期、T分期、分化等级有关(P<0.05),与性别、年龄、部位、吸烟史、淋巴结转移无关(P>0.05)。ROC曲线显示:以9.0 FU/3 mL为血清FR⁺-CTCs的最佳cut-off值诊断LSCC,曲线下面积(area under curve, AUC)为0.857,其敏感度为76.2%,特异度为95.5%。结论 LSCC患者外周血FR⁺-CTCs水平较喉良性病变患者升高,且增高程度与临床分期及分化等级相关,FR⁺-CTCs检测有望应用于LSCC的临床诊断。

关键词: 喉癌, 叶酸受体, 循环肿瘤细胞, 血清, 生物标志物

Abstract: Objective To determine serum levels of folate receptor-positive circulating tumor cells(FR⁺-CTCs)in patients with laryngeal squamous cell carcinoma(LSCC)and investigate its utility in the diagnosis of LSCC. Methods Forty-two patients with LSCC and 22 with benign laryngeal lesions admitted to the authors’ hospital were enrolled in this study. The significance of peripheral blood levels of FR⁺-CTCs, combined with clinical characteristics, in the diagnosis of LSCC was analyzed. Results FR⁺-CTC levels in patients with LSCC were higher than those in the benign group(t=7.269, P<0.001). Additionally, FR⁺-CTC level was associated with clinical stage, T stage, and differentiation grade(P<0.05), but not with sex, age, location, smoking, or lymph node metastasis(P>0.05). The area under the receiver operating characteristic curve was 0.857; sensitivity was 76.2% and specificity was 95.5% for the detection of LSCC with 9.0 FU/3 mL as the cut-off value. Conclusion Peripheral blood levels of FR⁺-CTCs in patients with LSCC were increased and was associated with clinical stage and differentiation grade. The determination of FR⁺-CTC levels is anticipated to be applied in the clinical diagnosis of LSCC.

Key words: Laryngeal cancer, Folate receptor, Circulating tumor cell, Serum, Biomarkers

中图分类号:

R739.6

张竹萍,叶琪,郭蓓,林凌. 叶酸受体阳性循环肿瘤细胞检测在喉鳞状细胞癌诊断中的应用价值[J]. 山东大学耳鼻喉眼学报, 2023, 37(5): 50-53.

ZHANG Zhuping, YE Qi, GUO Bei, LIN Ling. Diagnostic utility of folate receptor-positive circulating tumor cells in patients with laryngeal squamous cell carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 50-53.

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