JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (1): 64-68.doi: 10.6040/j.issn.1673-3770.0.2013.268

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Collaterals therapy influences the expression of VCAM-1 in the retinal capillary wall of STZ-induced diabetic rats

MA Dong, GUO Cheng-wei   

  1. Department of Ophthalmology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, Shandong, China
  • Received:2013-09-22 Online:2015-02-16 Published:2015-02-16

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Abstract: Objective To observe the expression of vascular cell adhesion molecule -1 (VCAM-1) in the retinal digest preparations of streptozotocin (STZ)-induced diabetic rats treated with collaterals therapy, and to investigate the protective effects and mechanism of collaterals therapy on diabetic retinopathy microvascular injury. Methods Sixty female Wistar rats were randomly divided into 4 groups including blank group, model group, control group and collaterals therapy group. All except the blank group rats were made into models of diabetic rats and treated according to the scheme. After 24 weeks each rat's bilateral eyes were made into retinal vascular digest preparations. The right eye was treated by PAS staining for morphological observation and the left eye by immunofluorescence staining for determining the VCAM-1 expression in the vessel wall. Results PAS staining showed that collaterals therapy group had obvious improvement on morphological changes such as vessel diameter, local expansion, vascular twist and so on. However, retinal pericyte damage of collaterals therapy group was significantly suppressed (P<0.05). Immunofluorescence staining showed that VCAM-1 in collaterals therapy group had less expression than in the other groups (P<0.05). Conclusion Collaterals therapy can inhibit the expression of VCAM-1 in diabetic retinal capillary wall, effectively alleviate retinal microvascular damage caused by hyperglycemia, and improve diabetic retinal microvascular structure and function.

Key words: Rhubarb eupolyphaga pills, Diabetic retinopathy, Vascular cell adhesion molecule-1, Collaterals therapy, Rats

CLC Number: 

  • R574
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