JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (3): 93-95.doi: 10.6040/j.issn.1673-3770.0.2015.517

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Effect of rh-bFGF on the level of glutamic acid in retinal tissue of rabbits with ischemia-reperfusion injury.

SHAO Hongchao, GE Yanran   

  1. Department of Ophthalmology, North China University of Sciense and Technology, Tangshan 063000, Hebei, China
  • Received:2015-12-07 Online:2016-06-16 Published:2016-06-16

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Abstract: Objective To explore the effect of recombinant human basic fibroblast growth factor(rh-bFGF)on the level of glutamic acid in retinal tissue of rabbits with ischemia-reperfusion injury. Methods Sixty-six white rabbits were divided into group A［retinal ischemia-reperfusion injury(RIRI)group, n=30］, group B(RIRI+rh-bFGF, n=30)and group C(sham-operation group, n=6). Groups A and B were also assigned into 5 subgroups respectively 6, 12, 24, 48 and 72 h after ischemia-reperfusion injury, 6 rabbits in each group. The same dose of normal saline and rh-bFGF solution were respectively injected into the vitreous cavity of rabbits in groups A and B at the early stage of molding. The rabbits were killed and their eyeballs were taken out at five time points after molding. Puncture of anterior chamber was given in group C, and no other operations were given. The rabbits in group C were killed and their eyeballs were taken out. The changes of retinal pathomorphism were observed, and the contents of glutamic acid were detected. Results In group A, the retina was characterized by high edema, disordered levels and loose cell structure. Vacuole degeneration was present in retinal ganglion cells(RGC)and and the number of ganglion cells decreased. In group B, the retinal structure changes were similar to that in A group, but the damage was significantly milder than that in group A. In group C, the levels of retina were clear, the cells were arranged in parallel and retinal morphology was reguLar. RGC was reguLarly arranged in a single layer, and no vacuole degeneration occurred. 6, 12, 24, 48 and 72 h after ischemia-reperfusion injury, the contents of glutamic acid in groups A and B were dramatically higher than that in group C(P<0.01). When compared with group A, the contents of glutamic acid in group B decreased notably at different time points(P<0.01). Conclusion rh-bFGF has a protective effect on retinal injury in rabbits, and its mechanism may be related to the inhibition of cell apoptosis and decreased content of glutamic acid.

Key words: Recombinant human basic fibroblast growth factor, Retina, Ischemia-reperfusion injury, Glutamic acid

CLC Number: 

  • R774.1
[1] Wang X D, Kashii S, Zhao L, et al. Vitamin B6 protects primate retinal neurons from ischemic injury[J]. Brain Res, 2002, 940(1-2):36-43.
[2] 杨丹, 余德立, 余资江. 视网膜缺血再灌注损伤的机制及药物治疗进展[J]. 局解手术学杂志, 2012, 21(5):561-563. YANG Dan, YU Deli, YU Zijiang. Progress of drug therapy for retinal ischemia reperfusion injury and its mechanisms[J]. J Reg Ana Ope Surgery, 2012, 21(5):561-563.
[3] 陈放, 徐珊, 吕伟红, 等. 糖尿病大鼠视网膜氧化应激损伤及葛根素的干预作用[J]. 眼科新进展, 2012, 32(1):15-19. CHEN Fang, XU Shan, L(¨overU)Weihong, et al. Retinal oxidative damage in diabetic rats and protective effect of puerarin on its[J]. Rec Adv Ophthalmol, 2012, 32(1):15-19.
[4] Vorwerk C K, Zurakowski D, McDermott L M, et al. Effects of axonal injury on ganglion cell survival and glutamate homeostasis[J]. Brain Res BuLl, 2004, 62(6):485-490.
[5] 刘朋朋, 张琦. 外源性H2S预处理对大鼠RIRI细胞凋亡的影响[J]. 国际眼科杂志, 2012, 12(1):33-35. LIU Pengpeng, ZHANG Qi. Effect of exogenous hydrogen suLfide preconditioning on cell apoptosis in rat retinal ischemia-reperfusion injury[J]. Inter J Ophthalmol, 2012, 12(1):33-35.
[6] 刘哲峰, 刘英奇, 张希兰, 等. 视网膜对缺血耐受时间的实验研究[J]. 眼科研究, 1988, 6(1):12-14.
[7] FouLds W S, Johnson N F. Rabbit electroretinogram during recovery from induced ischaemia[J]. Trans Ophthalmol Soc U K, 1974, 94(2):383-393.
[8] 李瑞恒, 王丽英, 王建平, 等. 复方樟柳碱联合葛根素治疗视网膜血管阻塞性疾病25例[J]. 山东大学耳鼻喉眼学报, 2010, 24(5):71-71, 75.
[9] Aikawa H, Tomita H, Ishiguro S, et al. Increased expression of glutamate binding protein mRNA in rat retina after ischemia-reperfusion injury[J]. Tohoku J Exp Med, 2003, 199(1):25-33.
[10] 包秀丽, 艾育德. 视网膜缺血性损伤中兴奋性氨基酸的变化[J]. 眼科新进展, 2005, 25(1):24-26. BAO Xiuli, AI Yude. Changes of excitatory amino acids in ischemic retinal damage[J].Rec Adv Ophthalmol, 2005, 25(1):24-26.
[11] 刘索新, 鞠学红. 神经营养因子对视网膜缺血再灌注损伤的影响[J]. 解剖科学进展, 2013, 19(5):454-457.
[12] 宋庆磊, 霍鸣. 评价视网膜缺血再灌注损伤指标的研究进展[J]. 国际眼科杂志, 2014, 34(9):1293-1296. SONG Qinglei, HUO Ming. Progress in evaluation indexs of retinal ischemia-reperfusion injury[J]. Basic Clin Med, 2014, 34(9):1293-1296.
[13] 姜义娜, 田福荣, 林倩, 等. bFGF 经鼻给药对大鼠缺血再灌注脑损伤的保护作用[J]. 温州医科大学学报, 2015, 45(8):560-565. JIANG Yina, TIAN Furong, LIN Qian, et al. Neruoprotective effect of bfgf on ischemic-reperfusion injury in sd rats via intranasal administration[J]. J Wenzhou Med Univ, 2015(8):560-565.
[14] 刘华, 陶宏军. 应用重组人碱性成纤维细胞生长因子论著治疗小儿面颈部烧伤的临床研究[J]. 临床和实验医学杂志, 2012, 11(1):31-32. LIU Hua, TAO Hongjun. A study of the treatment of 67 cases of face and neck burns in children[J]. J Clin Exp Med, 2012, 11(1):31-32.
[15] 车莹, 李校堃, 胡培声, 等. 重组人碱性成纤细胞生长因子单克隆抗体的制备、鉴定及初步应用[J].免疫学杂志, 2011, 27(6):514-518.
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