JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (2): 67-72.doi: 10.6040/j.issn.1673-3770.0.2016.425

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Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma.

  

  1. 1.Department of Otorhinolaryngology, First Affiliated Hospital of Nanjing Medical University/Jiangsu Provincial Peoples Hospital, Nanjing 210029, Jiangsu, China;2.Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, China
  • Received:2016-10-09 Online:2017-04-16 Published:2017-04-16

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Abstract: Objective To study the effect of curcumin in combination of resveratrol on proliferation in the head and neck neoplasm cell lines. Methods Hep2 cells and FaDu cells were treated with Curcumin and resveratrol. MTT was used to determine cell proliferation. Colony formation was used to detect the cell viability. Hoechst 33258 staining was performed to observe the cell apoptosis. The mRNA expressions of Bax and Bcl-2 were analyzed by Real-time PCR. Results Hep2 and FaDu cell proliferation could be inhibited with curcumin and resveratrol in a dose-dependent manner. Their combination exhibited the best inhibitory effects. Curcumin combined with resveratrol exhibited colony repression and showed a more significant apoptosis in Hep2 and FaDu cells. Real-time PCR demonstrated that Bax mRNA was upregulated and Bcl-2 mRNA was downregulated by curcumin and resveratrol. Meanwhile, Bax mRNA was upregulated and Bcl-2 mRNA was downregulated by curcumin and resveratrol. Conclusion The combination of curcumin and resveratrol can inhibit the proliferation of Hep2 and FaDu cells more significantly. The potential mechanism may be related with the up-regulation of Bax and the down-regulation of Bcl-2.

Key words: Proliferation, Curcumin, Resveratrol, Hep2 cells, FaDu cells, Apoptosis

CLC Number: 

  • R739.91
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