Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (4): 66-70.doi: 10.6040/j.issn.1673-3770.0.2018.409

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Effects of lncRNA CTB-147C22.8 and mRNA KLK6 on the invasion of recurrent respiratory papilloma cells

PAN Xiaofei, WANG Jun, XIAO Yang, MA Lijing   

  1. Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital affiliated to Capital Medical University, Key Laboratory of Otolaryngology, Head and Neck Science, Ministry of Education, Capital Medical University, Beijing 100730, China
  • Online:2019-07-20 Published:2019-07-22

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Abstract: Objective To investigate the effect of lncRNA CTB-147C22.8 on the invasion of recurrent respiratory papilloma cells. Methods A high-throughput genome expression profile chip was used for early stage analysis. To establish a human nasopharyngeal epithelial cell line(NP69 - SV40T), cells were incubated with Lipofectamine TM2000 for transfection; the transfected nasopharyngeal epithelial cells contained the exogenous gene recombinant plasmid pcDNA3.1/zeo+-CTB-147C22.8. Based on the transfection carrier, cells were divided into three groups: NP69 blank group, NP69 H145 group, and NP69-H6373 group. NP69 indicates untransfected plasmid cell blank group, NP69 H145 group refers to the cells transfected with a blank plasmid, and NP69 H6373 group refers to the cells transfected with the target gene CTB-147C22.8. KLK6 expression in human nasopharyngeal epithelial cells before and after transfection of lncRNA CTB-147C22.8 was detected by qrt-pcr. Flow cytometry was performed on the epithelial cells transfected with lncRNA CTB-147C22.8, and cell cycle analysis was performed by using FlowJo software. Transwell assay was used to determine the effect of lncRNA CTB-147C22.8 on cell invasion. Results KLK6 gene expression levels in the NP69-blank, NP69-H145, and NP69 H6373 groups were 1.005 771 107±0.008 724 979, 1.591 421 113±0.311 999 127, and 1.880 766 963±0.109 759 031, respectively. Compared with NP69-blank and NP69-H145 groups, the duration of cells in S stage in the NP69 H6373 group was significantly longer(F=8.763, P=0.017). The number of cells migrating in the NP69 H6373, NP69-H145, and NP69 H6373 groups was 6.889±2.261, 8.222±3.114, and 39.556±18.228, respectively. The differences in these numbers were statistically significant between the NP69 H6373 group, and the NP69-blank group and NP69-H145 group(F=3.038, P=0.034). Conclusion lncRNA CTB-147C22.8 promotes the invasion of recurrent respiratory papilloma cells.

Key words: Recurrent respiratory papillomatosis, lncRNA, KLK6, Invasion

CLC Number: 

  • R734
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